Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,330 T→C 17.2% intergenic (+98/‑1681) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3300TC17.2% 145.1 / 22.4 64intergenic (+98/‑1681)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/35);  new base C (8/3);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

CTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGA  >  minE/2571261‑2571399
                                                                      |                                                                      
cTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTa                                                                       <  1:737063/71‑1 (MQ=255)
   cTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAtt                                                                        <  1:848762/67‑1 (MQ=255)
      gTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                                 >  1:450382/1‑71 (MQ=255)
       tAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                                 <  1:921862/70‑1 (MQ=255)
        aaGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaat                                                                <  1:3318810/70‑1 (MQ=255)
         aGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                              <  1:2593480/71‑1 (MQ=255)
          ggACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAg                                                             <  1:78859/71‑1 (MQ=255)
           gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:1057921/71‑1 (MQ=255)
           gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:707929/71‑1 (MQ=255)
            aCTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:707223/70‑1 (MQ=255)
              tAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                                 >  1:3075837/1‑63 (MQ=255)
                attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaata                                                               >  1:106744/1‑63 (MQ=255)
                attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                       <  1:2119564/70‑1 (MQ=255)
                 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGt                                                                    >  1:477513/1‑57 (MQ=255)
                 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                     <  1:1232140/71‑1 (MQ=255)
                 ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                     <  1:2225942/71‑1 (MQ=255)
                  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                    >  1:418124/1‑71 (MQ=255)
                  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                    >  1:85200/1‑71 (MQ=255)
                  tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                    >  1:2182597/1‑71 (MQ=255)
                   attTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                    >  1:2254309/1‑70 (MQ=255)
                    tttttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATg                                                   <  1:3533469/70‑1 (MQ=255)
                      tttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAg                                                 <  1:2502571/70‑1 (MQ=255)
                        tGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtg                                               <  1:2389877/70‑1 (MQ=255)
                         gCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtgt                                              >  1:1360047/1‑70 (MQ=255)
                           atatTACTTCATTAGCTTCGATTATTTTTCTTTAATATTCATTATGTTAATAAGtt                                                           >  1:1502246/1‑56 (MQ=255)
                           atatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTAct                                           <  1:2694549/71‑1 (MQ=255)
                             atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaata                                                               <  1:752619/50‑1 (MQ=255)
                              ttACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                         <  1:175902/70‑1 (MQ=255)
                                aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                       <  1:3027968/70‑1 (MQ=255)
                                aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                       <  1:1985376/70‑1 (MQ=255)
                                 cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                              >  1:1194867/1‑47 (MQ=255)
                                 cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCAtt                                     <  1:2485622/71‑1 (MQ=255)
                                    cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                     >  1:1637292/1‑52 (MQ=255)
                                    cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTg                                    <  1:2992931/69‑1 (MQ=255)
                                    cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGa                                  >  1:2008979/1‑71 (MQ=255)
                                     aTTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGGGTGCTCTCATTGGAt                                 <  1:2791507/71‑1 (MQ=255)
                                      ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAg                                                             <  1:2789876/43‑1 (MQ=255)
                                      ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                       <  1:1884285/48‑1 (MQ=255)
                                      ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                       <  1:909931/48‑1 (MQ=255)
                                        aGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                     >  1:2105731/1‑48 (MQ=255)
                                          cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:1809107/71‑1 (MQ=255)
                                          cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:1410050/71‑1 (MQ=255)
                                          cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:51191/71‑1 (MQ=255)
                                            tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                >  1:1241951/1‑49 (MQ=255)
                                            tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                >  1:2625243/1‑49 (MQ=255)
                                            gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCt                                                        >  1:1733264/3‑42 (MQ=12)
                                            gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCTg                                                       >  1:2571275/3‑43 (MQ=16)
                                             cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                         >  1:466854/1‑71 (MQ=255)
                                              gatgatTTTTCCTTTAATATCCAGCATGTAAAGAAGTTc                                                          >  1:2962167/1‑39 (MQ=9)
                                               atgatTTTTCTTTAATATTCATTATGTTAATAAGtt                                                           <  1:1363014/36‑1 (MQ=255)
                                               atgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                       <  1:1035998/71‑1 (MQ=255)
                                                tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                        >  1:602030/1‑71 (MQ=255)
                                                 gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                     >  1:334079/1‑71 (MQ=255)
                                                  atTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                     <  1:833991/70‑1 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                     >  1:1663272/3‑67 (MQ=255)
                                                       tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                  <  1:1699436/68‑1 (MQ=255)
                                                        ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga                 <  1:2002593/69‑2 (MQ=255)
                                                         cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc               <  1:681628/71‑1 (MQ=255)
                                                         atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc               >  1:1204721/2‑71 (MQ=255)
                                                           ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg             >  1:1385251/1‑71 (MQ=255)
                                                            tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt            >  1:3859165/1‑70 (MQ=255)
                                                               tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAt                                 <  1:2954658/46‑1 (MQ=255)
                                                               tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc         <  1:1658843/70‑1 (MQ=255)
                                                                    aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt    <  1:3677991/70‑1 (MQ=255)
                                                                    aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt    <  1:2701468/70‑1 (MQ=255)
                                                                     ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga  <  1:2009661/71‑1 (MQ=255)
                                                                      tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc               >  1:779794/1‑57 (MQ=255)
                                                                      tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa     >  1:2418661/1‑67 (MQ=255)
                                                                      |                                                                      
CTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGA  >  minE/2571261‑2571399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: