Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,338 T→G 20.7% intergenic (+106/‑1673) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3380TG20.7% 98.3 / 35.6 63intergenic (+106/‑1673)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (16/34);  new base G (8/5);  total (24/39)
Fisher's exact test for biased strand distribution p-value = 6.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

AAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACA  >  minE/2571269‑2571408
                                                                      |                                                                       
aaGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaat                                                                         <  1:3318810/70‑1 (MQ=255)
 aGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaataa                                                                       <  1:2593480/71‑1 (MQ=255)
  ggACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAg                                                                      <  1:78859/71‑1 (MQ=255)
   gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                                     <  1:1057921/71‑1 (MQ=255)
   gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                                     <  1:707929/71‑1 (MQ=255)
    aCTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGt                                                                     <  1:707223/70‑1 (MQ=255)
        attattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaata                                                                        >  1:106744/1‑63 (MQ=255)
        attattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                                <  1:2119564/70‑1 (MQ=255)
         ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                                              <  1:1232140/71‑1 (MQ=255)
         ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                                              <  1:2225942/71‑1 (MQ=255)
          tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                                             >  1:85200/1‑71 (MQ=255)
          tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                                             >  1:2182597/1‑71 (MQ=255)
          tattTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                                             >  1:418124/1‑71 (MQ=255)
           attTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGAt                                                             >  1:2254309/1‑70 (MQ=255)
            tttttGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATg                                                            <  1:3533469/70‑1 (MQ=255)
              tttGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAg                                                          <  1:2502571/70‑1 (MQ=255)
                tGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgtg                                                        <  1:2389877/70‑1 (MQ=255)
                 gCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgtgt                                                       >  1:1360047/1‑70 (MQ=255)
                   atatTACTTCATTAGCTTCGATTATTTTT‑CTTTAATATTCATTATGTTAATAAGtt                                                                    >  1:1502246/1‑56 (MQ=255)
                   atatTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTAct                                                    <  1:2694549/71‑1 (MQ=255)
                     atTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaata                                                                        <  1:752619/50‑1 (MQ=255)
                      ttACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGctct                                                  <  1:175902/70‑1 (MQ=255)
                        aCTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCa                                                <  1:1985376/70‑1 (MQ=255)
                        aCTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCa                                                <  1:3027968/70‑1 (MQ=255)
                         cTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTtaataa                                                                       >  1:1194867/1‑47 (MQ=255)
                         cTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCAtt                                              <  1:2485622/71‑1 (MQ=255)
                            cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                                              >  1:1637292/1‑52 (MQ=255)
                            cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTg                                             <  1:2992931/69‑1 (MQ=255)
                            cATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGa                                           >  1:2008979/1‑71 (MQ=255)
                             aTTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGGGTGCTCTCATTGGAt                                          <  1:2791507/71‑1 (MQ=255)
                              ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAg                                                                      <  1:2789876/43‑1 (MQ=255)
                              ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                                <  1:909931/48‑1 (MQ=255)
                              ttAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑Tg                                                                <  1:1884285/48‑1 (MQ=255)
                                aGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGa                                                              >  1:2105731/1‑48 (MQ=255)
                                  cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                                     <  1:51191/71‑1 (MQ=255)
                                  cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                                     <  1:1410050/71‑1 (MQ=255)
                                  cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt                                     <  1:1809107/71‑1 (MQ=255)
                                    tCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgt                                                         >  1:1241951/1‑49 (MQ=255)
                                    tCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAgt                                                         >  1:2625243/1‑49 (MQ=255)
                                    gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTC‑t                                                                 >  1:1733264/3‑42 (MQ=12)
                                    gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTC‑Tg                                                                >  1:2571275/3‑43 (MQ=16)
                                     cGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATc                                  >  1:466854/1‑71 (MQ=255)
                                      gatgatTTTTCCTTTAATATCCAGCATGTAAAGAAGTTc                                                                   >  1:2962167/1‑39 (MQ=9)
                                       atgatTTTT‑CTTTAATATTCATTATGTTAATAAGtt                                                                    <  1:1363014/36‑1 (MQ=255)
                                       atgatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                                <  1:1035998/71‑1 (MQ=255)
                                        tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                                 >  1:602030/1‑71 (MQ=255)
                                         gatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                              >  1:334079/1‑71 (MQ=255)
                                          atTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                              <  1:833991/70‑1 (MQ=255)
                                               tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                              >  1:1663272/3‑67 (MQ=255)
                                               tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                           <  1:1699436/68‑1 (MQ=255)
                                                ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga                          <  1:2002593/69‑2 (MQ=255)
                                                 cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc                        <  1:681628/71‑1 (MQ=255)
                                                 atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                        >  1:1204721/2‑71 (MQ=255)
                                                   ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg                      >  1:1385251/1‑71 (MQ=255)
                                                    tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt                     >  1:3859165/1‑70 (MQ=255)
                                                       tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAt                                          <  1:2954658/46‑1 (MQ=255)
                                                       tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc                  <  1:1658843/70‑1 (MQ=255)
                                                            aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt             <  1:3677991/70‑1 (MQ=255)
                                                            aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt             <  1:2701468/70‑1 (MQ=255)
                                                             ttATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga           <  1:2009661/71‑1 (MQ=255)
                                                             gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                    <  1:3193494/45‑1 (MQ=255)
                                                             gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                    <  1:3041316/45‑1 (MQ=255)
                                                              tATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc                        >  1:779794/1‑57 (MQ=255)
                                                              tATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa              >  1:2418661/1‑67 (MQ=255)
                                                               aTGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgag          <  1:3209317/70‑1 (MQ=255)
                                                                tGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc        >  1:355609/1‑71 (MQ=255)
                                                                  ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt       <  1:209001/70‑1 (MQ=255)
                                                                  ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt       <  1:3269259/70‑1 (MQ=255)
                                                                    aataaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat     <  1:257301/70‑1 (MQ=255)
                                                                     ataaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata    <  1:2815788/70‑1 (MQ=255)
                                                                      taaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa  >  1:13632/1‑71 (MQ=255)
                                                                      |                                                                       
AAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACA  >  minE/2571269‑2571408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑