Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,338 T→G 20.7% intergenic (+106/‑1673) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3380TG20.7% 98.3 / 35.6 63intergenic (+106/‑1673)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (16/34);  new base G (8/5);  total (24/39)
Fisher's exact test for biased strand distribution p-value = 6.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

AAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACA  >  minE/2571269‑2571408
                                                                      |                                                                       
aaGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaat                                                                         <  1:3318810/70‑1 (MQ=255)
 aGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                                       <  1:2593480/71‑1 (MQ=255)
  ggACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAg                                                                      <  1:78859/71‑1 (MQ=255)
   gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                                     <  1:1057921/71‑1 (MQ=255)
   gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                                     <  1:707929/71‑1 (MQ=255)
    aCTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                                     <  1:707223/70‑1 (MQ=255)
        attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaata                                                                        >  1:106744/1‑63 (MQ=255)
        attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                                <  1:2119564/70‑1 (MQ=255)
         ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                              <  1:1232140/71‑1 (MQ=255)
         ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                              <  1:2225942/71‑1 (MQ=255)
          tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                             >  1:85200/1‑71 (MQ=255)
          tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                             >  1:2182597/1‑71 (MQ=255)
          tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                             >  1:418124/1‑71 (MQ=255)
           attTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                             >  1:2254309/1‑70 (MQ=255)
            tttttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATg                                                            <  1:3533469/70‑1 (MQ=255)
              tttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAg                                                          <  1:2502571/70‑1 (MQ=255)
                tGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtg                                                        <  1:2389877/70‑1 (MQ=255)
                 gCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtgt                                                       >  1:1360047/1‑70 (MQ=255)
                   atatTACTTCATTAGCTTCGATTATTTTTCTTTAATATTCATTATGTTAATAAGtt                                                                    >  1:1502246/1‑56 (MQ=255)
                   atatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTAct                                                    <  1:2694549/71‑1 (MQ=255)
                     atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaata                                                                        <  1:752619/50‑1 (MQ=255)
                      ttACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                                  <  1:175902/70‑1 (MQ=255)
                        aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                                <  1:1985376/70‑1 (MQ=255)
                        aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                                <  1:3027968/70‑1 (MQ=255)
                         cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                                       >  1:1194867/1‑47 (MQ=255)
                         cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCAtt                                              <  1:2485622/71‑1 (MQ=255)
                            cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                              >  1:1637292/1‑52 (MQ=255)
                            cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTg                                             <  1:2992931/69‑1 (MQ=255)
                            cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGa                                           >  1:2008979/1‑71 (MQ=255)
                             aTTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGGGTGCTCTCATTGGAt                                          <  1:2791507/71‑1 (MQ=255)
                              ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAg                                                                      <  1:2789876/43‑1 (MQ=255)
                              ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                                <  1:909931/48‑1 (MQ=255)
                              ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                                <  1:1884285/48‑1 (MQ=255)
                                aGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                              >  1:2105731/1‑48 (MQ=255)
                                  cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                     <  1:51191/71‑1 (MQ=255)
                                  cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                     <  1:1410050/71‑1 (MQ=255)
                                  cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                     <  1:1809107/71‑1 (MQ=255)
                                    tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                         >  1:1241951/1‑49 (MQ=255)
                                    tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                         >  1:2625243/1‑49 (MQ=255)
                                    gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCt                                                                 >  1:1733264/3‑42 (MQ=12)
                                    gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCTg                                                                >  1:2571275/3‑43 (MQ=16)
                                     cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                                  >  1:466854/1‑71 (MQ=255)
                                      gatgatTTTTCCTTTAATATCCAGCATGTAAAGAAGTTc                                                                   >  1:2962167/1‑39 (MQ=9)
                                       atgatTTTTCTTTAATATTCATTATGTTAATAAGtt                                                                    <  1:1363014/36‑1 (MQ=255)
                                       atgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                                <  1:1035998/71‑1 (MQ=255)
                                        tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                                 >  1:602030/1‑71 (MQ=255)
                                         gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                              >  1:334079/1‑71 (MQ=255)
                                          atTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                              <  1:833991/70‑1 (MQ=255)
                                               tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                              >  1:1663272/3‑67 (MQ=255)
                                               tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                           <  1:1699436/68‑1 (MQ=255)
                                                ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga                          <  1:2002593/69‑2 (MQ=255)
                                                 cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc                        <  1:681628/71‑1 (MQ=255)
                                                 atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                        >  1:1204721/2‑71 (MQ=255)
                                                   ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg                      >  1:1385251/1‑71 (MQ=255)
                                                    tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt                     >  1:3859165/1‑70 (MQ=255)
                                                       tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAt                                          <  1:2954658/46‑1 (MQ=255)
                                                       tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc                  <  1:1658843/70‑1 (MQ=255)
                                                            aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt             <  1:3677991/70‑1 (MQ=255)
                                                            aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt             <  1:2701468/70‑1 (MQ=255)
                                                             ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga           <  1:2009661/71‑1 (MQ=255)
                                                             gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                    <  1:3193494/45‑1 (MQ=255)
                                                             gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                    <  1:3041316/45‑1 (MQ=255)
                                                              tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc                        >  1:779794/1‑57 (MQ=255)
                                                              tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa              >  1:2418661/1‑67 (MQ=255)
                                                               aTGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgag          <  1:3209317/70‑1 (MQ=255)
                                                                tGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc        >  1:355609/1‑71 (MQ=255)
                                                                  ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt       <  1:209001/70‑1 (MQ=255)
                                                                  ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt       <  1:3269259/70‑1 (MQ=255)
                                                                    aataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat     <  1:257301/70‑1 (MQ=255)
                                                                     ataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata    <  1:2815788/70‑1 (MQ=255)
                                                                      taaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa  >  1:13632/1‑71 (MQ=255)
                                                                      |                                                                       
AAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACA  >  minE/2571269‑2571408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: