Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,350 G→A 19.6% intergenic (+118/‑1661) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3500GA19.6% 108.1 / 19.7 51intergenic (+118/‑1661)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (13/28);  new base A (5/5);  total (18/33)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

TTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG  >  minE/2571281‑2571420
                                                                       |                                                                      
tttttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATg                                                                        <  1:3533469/70‑1 (MQ=255)
  tttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAg                                                                      <  1:2502571/70‑1 (MQ=255)
    tGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtg                                                                    <  1:2389877/70‑1 (MQ=255)
     gCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtgt                                                                   >  1:1360047/1‑70 (MQ=255)
       atatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTAct                                                                <  1:2694549/71‑1 (MQ=255)
          ttACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                                              <  1:175902/70‑1 (MQ=255)
            aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                                            <  1:3027968/70‑1 (MQ=255)
            aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                                            <  1:1985376/70‑1 (MQ=255)
             cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCAtt                                                          <  1:2485622/71‑1 (MQ=255)
                cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTg                                                         <  1:2992931/69‑1 (MQ=255)
                cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGa                                                       >  1:2008979/1‑71 (MQ=255)
                 aTTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGGGTGCTCTCATTGGAt                                                      <  1:2791507/71‑1 (MQ=255)
                      cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                                 <  1:1410050/71‑1 (MQ=255)
                      cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                                 <  1:1809107/71‑1 (MQ=255)
                      cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                                                 <  1:51191/71‑1 (MQ=255)
                        tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                                     >  1:2625243/1‑49 (MQ=255)
                        tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                                     >  1:1241951/1‑49 (MQ=255)
                         cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                                              >  1:466854/1‑71 (MQ=255)
                           atgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                                            <  1:1035998/71‑1 (MQ=255)
                            tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                                             >  1:602030/1‑71 (MQ=255)
                             gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                          >  1:334079/1‑71 (MQ=255)
                              atTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                          <  1:833991/70‑1 (MQ=255)
                                   tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                                          >  1:1663272/3‑67 (MQ=255)
                                   tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                       <  1:1699436/68‑1 (MQ=255)
                                    ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga                                      <  1:2002593/69‑2 (MQ=255)
                                     cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc                                    <  1:681628/71‑1 (MQ=255)
                                     atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                                    >  1:1204721/2‑71 (MQ=255)
                                       ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg                                  >  1:1385251/1‑71 (MQ=255)
                                        tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt                                 >  1:3859165/1‑70 (MQ=255)
                                           tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAt                                                      <  1:2954658/46‑1 (MQ=255)
                                           tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc                              <  1:1658843/70‑1 (MQ=255)
                                                aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                         <  1:3677991/70‑1 (MQ=255)
                                                aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                         <  1:2701468/70‑1 (MQ=255)
                                                 ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                       <  1:2009661/71‑1 (MQ=255)
                                                 gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                                <  1:3041316/45‑1 (MQ=255)
                                                 gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                                <  1:3193494/45‑1 (MQ=255)
                                                  tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc                                    >  1:779794/1‑57 (MQ=255)
                                                  tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa                          >  1:2418661/1‑67 (MQ=255)
                                                   aTGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgag                      <  1:3209317/70‑1 (MQ=255)
                                                    tGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc                    >  1:355609/1‑71 (MQ=255)
                                                      ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt                   <  1:3269259/70‑1 (MQ=255)
                                                      ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt                   <  1:209001/70‑1 (MQ=255)
                                                        aataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat                 <  1:257301/70‑1 (MQ=255)
                                                         ataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata                <  1:2815788/70‑1 (MQ=255)
                                                          taaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa              >  1:13632/1‑71 (MQ=255)
                                                           aaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAt             >  1:3522116/1‑71 (MQ=255)
                                                             gTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcg           <  1:1944225/71‑1 (MQ=255)
                                                                  tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa                          >  1:506791/1‑52 (MQ=255)
                                                                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                       <  1:3675681/53‑1 (MQ=255)
                                                                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat                 >  1:3271316/1‑59 (MQ=255)
                                                                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag      <  1:2084695/70‑1 (MQ=255)
                                                                    gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga       <  1:3808151/69‑1 (MQ=255)
                                                                       gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg  >  1:869814/1‑71 (MQ=255)
                                                                       |                                                                      
TTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG  >  minE/2571281‑2571420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: