| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,571,371 | T→C | 26.7% | intergenic (+139/‑1640) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,571,371 | 0 | T | C | 26.7% | 91.7 / 38.6 | 60 | intergenic (+139/‑1640) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (16/28); new base C (6/10); total (22/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTT > minE/2571303‑2571440 | cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt < 1:1809107/71‑1 (MQ=255)cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt < 1:51191/71‑1 (MQ=255)cTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGAtttttt < 1:1410050/71‑1 (MQ=255) cGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATc > 1:466854/1‑71 (MQ=255) atgatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCg < 1:1035998/71‑1 (MQ=255) tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc > 1:602030/1‑71 (MQ=255) gatTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc > 1:334079/1‑71 (MQ=255) atTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc < 1:833991/70‑1 (MQ=255) tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc > 1:1663272/3‑67 (MQ=255) tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg < 1:1699436/68‑1 (MQ=255) ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga < 1:2002593/69‑2 (MQ=255) cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc < 1:681628/71‑1 (MQ=255) atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc > 1:1204721/2‑71 (MQ=255) ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg > 1:1385251/1‑71 (MQ=255) tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt > 1:3859165/1‑70 (MQ=255) tatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc < 1:1658843/70‑1 (MQ=255) aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt < 1:2701468/70‑1 (MQ=255) aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt < 1:3677991/70‑1 (MQ=255) ttATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:2009661/71‑1 (MQ=255) gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa < 1:3041316/45‑1 (MQ=255) gcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa < 1:3193494/45‑1 (MQ=255) tATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc > 1:779794/1‑57 (MQ=255) tATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa > 1:2418661/1‑67 (MQ=255) aTGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgag < 1:3209317/70‑1 (MQ=255) tGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc > 1:355609/1‑71 (MQ=255) ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:209001/70‑1 (MQ=255) ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:3269259/70‑1 (MQ=255) aataaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat < 1:257301/70‑1 (MQ=255) ataaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata < 1:2815788/70‑1 (MQ=255) taaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa > 1:13632/1‑71 (MQ=255) aaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAt > 1:3522116/1‑71 (MQ=255) gTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcg < 1:1944225/71‑1 (MQ=255) tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa > 1:506791/1‑52 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:3675681/53‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat > 1:3271316/1‑59 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:2084695/70‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:3808151/69‑1 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:869814/1‑71 (MQ=255) tgtgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:2270/47‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat > 1:3041879/1‑70 (MQ=255) ctctCATTGGATTTTTTATCCGTCTTGTTCTGCCGCa < 1:3611129/37‑1 (MQ=255) tctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGa > 1:3002213/1‑39 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgaga > 1:2672705/1‑42 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:1372972/43‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:254577/43‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:3461047/1‑63 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:29333/1‑71 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:2517521/68‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat < 1:3235391/71‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat < 1:3512756/71‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata < 1:1621088/70‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATgatgat < 1:2860854/68‑1 (MQ=255) ttGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:2080036/1‑70 (MQ=255) ttGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATg < 1:260330/71‑1 (MQ=255) ttGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATg < 1:1646094/71‑1 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3469100/50‑1 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3769408/50‑1 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat > 1:1556753/1‑69 (MQ=255) ggATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:1184069/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACGAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:2467650/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:1156558/70‑1 (MQ=255) gATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgt < 1:1932645/71‑1 (MQ=255) gATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg < 1:1563958/70‑1 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttg < 1:670240/71‑1 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATCAGACTATACATCGTCGAGATCGCAATGATGATAAGAgttg > 1:2667978/1‑71 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttg < 1:3012631/70‑1 (MQ=255) ttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttgtt > 1:2647521/1‑71 (MQ=255) | CTTCGATGATTTTT‑CTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTT > minE/2571303‑2571440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |