| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,571,398 | G→C | 9.3% | intergenic (+166/‑1613) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,571,398 | 0 | G | C | 9.3% | 180.5 / 6.2 | 64 | intergenic (+166/‑1613) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (18/40); new base C (2/4); total (20/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
TTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAA > minE/2571329‑2571467 | ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:2009661/71‑1 (MQ=255) aTGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgag < 1:3209317/70‑1 (MQ=255) tGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc > 1:355609/1‑71 (MQ=255) ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:209001/70‑1 (MQ=255) ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:3269259/70‑1 (MQ=255) aataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat < 1:257301/70‑1 (MQ=255) ataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata < 1:2815788/70‑1 (MQ=255) taaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa > 1:13632/1‑71 (MQ=255) aaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAt > 1:3522116/1‑71 (MQ=255) gTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcg < 1:1944225/71‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:3675681/53‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtat > 1:3271316/1‑59 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:2084695/70‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:3808151/69‑1 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:869814/1‑71 (MQ=255) tgtgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:2270/47‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat > 1:3041879/1‑70 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATgaga > 1:2672705/1‑42 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:254577/43‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:1372972/43‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:3461047/1‑63 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:29333/1‑71 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:2517521/68‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat < 1:3512756/71‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat < 1:3235391/71‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata < 1:1621088/70‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATgatgat < 1:2860854/68‑1 (MQ=255) ttGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:2080036/1‑70 (MQ=255) ttGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATg < 1:1646094/71‑1 (MQ=255) ttGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATg < 1:260330/71‑1 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3469100/50‑1 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3769408/50‑1 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat > 1:1556753/1‑69 (MQ=255) ggATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:1184069/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACGAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:2467650/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:1156558/70‑1 (MQ=255) gATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgt < 1:1932645/71‑1 (MQ=255) gATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg < 1:1563958/70‑1 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttg < 1:670240/71‑1 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATCAGACTATACATCGTCGAGATCGCAATGATGATAAGAgttg > 1:2667978/1‑71 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttg < 1:3012631/70‑1 (MQ=255) ttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttgtt > 1:2647521/1‑71 (MQ=255) ttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc > 1:1731894/1‑42 (MQ=255) tATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcc < 1:234069/70‑1 (MQ=255) aTCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtc > 1:332396/1‑68 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcctcc < 1:3341236/71‑1 (MQ=255) ccGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:2739097/52‑1 (MQ=255) ccGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:3576466/52‑1 (MQ=255) tCTTGTTCTGCCGCTGGATGAGACTATACATCGTCGAGATCGCAatga < 1:3886979/48‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGc < 1:592404/43‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:3506040/50‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:2090128/50‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga < 1:453366/48‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga < 1:1473688/48‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGc > 1:419092/1‑70 (MQ=255) cTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg < 1:14261/41‑1 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCga > 1:3343464/1‑35 (MQ=255) gTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga < 1:424019/44‑1 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACgg < 1:2401273/70‑1 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGct < 1:1130166/70‑1 (MQ=255) gCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttg > 1:1463725/1‑51 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTa < 1:2329925/70‑1 (MQ=255) cGCAGGATGAGACTATACATCGTCGAGATAGCAATGATGATATGAGTTGTtc > 1:1663616/1‑52 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaa < 1:1154003/69‑1 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGatat < 1:3056983/36‑1 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGatat < 1:3935969/36‑1 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttg > 1:1612552/1‑42 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttg > 1:2824578/1‑42 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaaaa < 1:217063/71‑1 (MQ=255) gagaCTATACATCGTCGAGATCGCAATGATGATATGAgttg < 1:1158905/41‑1 (MQ=255) gagaCTATACATCGTCGAGATCGCAATGATGATATGAgttg < 1:3501180/41‑1 (MQ=255) gagaCTATACATCGTCGAGATCGCAATGATGATATGAgttg < 1:1006055/41‑1 (MQ=255) gagaCTATACATCGTCGAGATCGCAATGATGATATGAg > 1:2470642/1‑38 (MQ=255) | TTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAA > minE/2571329‑2571467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |