| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,691 | T→C | 22.5% | T394T (ACA→ACG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,691 | 0 | T | C | 22.5% | 258.9 / 61.6 | 111 | T394T (ACA→ACG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (36/50); new base C (15/10); total (51/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
CTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAC > minE/311622‑311759 | cTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACAtt < 1:1341490/70‑1 (MQ=255) aaCGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGtt < 1:2023695/70‑1 (MQ=255) aaCGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTa < 1:692119/71‑1 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc < 1:848997/71‑1 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc > 1:1053744/1‑71 (MQ=255) gTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCg > 1:992196/1‑71 (MQ=255) gTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCg < 1:2724515/71‑1 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt > 1:2238548/1‑71 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt > 1:282127/1‑71 (MQ=255) tCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGtt > 1:3860039/1‑71 (MQ=255) tACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTa > 1:3897047/1‑71 (MQ=255) tACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTa > 1:1699142/1‑71 (MQ=255) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttga > 1:3350701/2‑69 (MQ=9) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttga > 1:482721/2‑69 (MQ=9) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttga > 1:93297/2‑69 (MQ=9) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttg < 1:193640/69‑2 (MQ=9) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttg < 1:1540903/69‑2 (MQ=9) accaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGAt < 1:1366210/71‑1 (MQ=9) accaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGAt > 1:2544236/1‑71 (MQ=9) aCCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt > 1:3452488/1‑71 (MQ=255) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt < 1:1304492/70‑1 (MQ=255) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATc < 1:1727597/71‑1 (MQ=255) cacaACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTa < 1:1515133/68‑1 (MQ=255) cacaACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCg < 1:564412/71‑1 (MQ=255) cacaACGATCGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCg < 1:1458078/71‑1 (MQ=255) caACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag < 1:2228932/71‑1 (MQ=255) aaCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga < 1:2189356/71‑1 (MQ=255) tGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc < 1:2971964/70‑1 (MQ=255) cGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAg < 1:3797414/71‑1 (MQ=255) atCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaaaag > 1:3276913/2‑69 (MQ=9) atCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaaaag > 1:2778838/2‑69 (MQ=9) tCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGtt < 1:1540298/53‑1 (MQ=16) tCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCg < 1:1899985/58‑1 (MQ=255) cATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAg > 1:227123/1‑62 (MQ=255) aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaaa > 1:563275/1‑66 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:1824706/71‑1 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:2575103/71‑1 (MQ=255) ccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGtt > 1:3401248/1‑46 (MQ=255) agcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAagcggc < 1:1648596/63‑1 (MQ=12) agcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat < 1:2299073/69‑2 (MQ=12) agcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat > 1:2319320/3‑70 (MQ=12) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc > 1:1262751/1‑39 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc > 1:426267/1‑39 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt < 1:3787862/47‑1 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGaa > 1:2471585/1‑53 (MQ=255) gcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGatg > 1:576654/2‑69 (MQ=12) gcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGatg > 1:3815727/2‑69 (MQ=12) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGtt > 1:2683269/1‑43 (MQ=255) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGtt > 1:1569027/1‑43 (MQ=255) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:3497243/1‑71 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACgg > 1:3534739/1‑70 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGc < 1:3191325/51‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:1796073/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2124814/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2363271/71‑1 (MQ=255) cccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCa < 1:690757/69‑1 (MQ=12) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga < 1:899035/46‑1 (MQ=255) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:1565651/69‑1 (MQ=255) cGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCaaac < 1:3652167/71‑2 (MQ=16) gATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:892493/1‑50 (MQ=9) gATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:1247340/1‑50 (MQ=9) gATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:2075371/1‑50 (MQ=9) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt > 1:1668109/1‑71 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt < 1:3732859/71‑1 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt > 1:820374/1‑71 (MQ=255) aTGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGAt > 1:3094098/1‑38 (MQ=17) tGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc > 1:1306873/1‑71 (MQ=255) tGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc > 1:2077061/1‑71 (MQ=255) tGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAagcgg > 1:3950389/1‑54 (MQ=9) ggCTAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:192406/1‑50 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:2907973/1‑58 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:762918/1‑60 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:3526958/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:2262432/71‑1 (MQ=255) aGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa < 1:772822/42‑1 (MQ=255) gCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAg > 1:2540071/1‑70 (MQ=255) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:3730817/1‑55 (MQ=255) aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa < 1:3568196/71‑1 (MQ=255) aCCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaa < 1:3435123/71‑1 (MQ=12) ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:1463810/46‑1 (MQ=255) aTCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGa > 1:3509140/1‑64 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggc < 1:170205/40‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc < 1:865345/52‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc < 1:2946875/52‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATac < 1:1866087/70‑1 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc < 1:1104027/58‑1 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATacca < 1:742993/70‑1 (MQ=255) cGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:2476207/53‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcg < 1:2982814/37‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGGCGGCAAAGGTCCCGAGCAATACCACCg > 1:3583996/1‑71 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg < 1:2780201/71‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg < 1:3451732/71‑1 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:2634466/71‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:899232/70‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg > 1:1436073/1‑70 (MQ=255) cATTGTTTGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa < 1:716698/71‑1 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa > 1:41513/1‑71 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa > 1:313318/1‑71 (MQ=255) aTTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAc > 1:3030104/1‑71 (MQ=255) tGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGa > 1:774512/1‑53 (MQ=255) | CTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAC > minE/311622‑311759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |