| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,708 | A→C | 31.5% | intergenic (+1476/‑303) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,708 | 0 | A | C | 31.5% | 52.7 / 68.8 | 70 | intergenic (+1476/‑303) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (27/21); new base C (15/7); total (42/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01 | |||||||||||
CAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTA > minE/2572639‑2572777 | cAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctac > 1:130073/1‑71 (MQ=255)cAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctac > 1:1423098/1‑71 (MQ=255) tGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctacc < 1:2517164/68‑1 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc > 1:3664811/1‑69 (MQ=255) cTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTACAg > 1:3191069/1‑71 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc < 1:2666618/71‑1 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGcc > 1:1950598/1‑70 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGc > 1:3826998/1‑69 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGcc > 1:3596932/1‑67 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa < 1:3388852/70‑1 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:2056839/1‑71 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:2024191/1‑71 (MQ=255) aGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:89579/1‑70 (MQ=255) ggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:2108127/56‑1 (MQ=255) ggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:1730317/56‑1 (MQ=255) tCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg < 1:783807/64‑1 (MQ=255) gATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctac < 1:3612179/47‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:3748041/1‑56 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt > 1:2295665/1‑70 (MQ=255) aGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGa < 1:2194973/64‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1563705/67‑1 (MQ=255) aaccGGATTCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAcc > 1:3568319/4‑71 (MQ=255) aaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAcc > 1:347736/1‑71 (MQ=255) ggattCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGccc > 1:1572421/4‑71 (MQ=255) ggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGtt > 1:779276/1‑61 (MQ=255) gattCAGCGGATACGCATACAGACTCCATCCCCCCACCAGCCCTATCGGGTCCTGAGTGATATACCGcccc > 1:1313014/3‑71 (MQ=255) attCAACGGATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGCCCct < 1:590119/70‑1 (MQ=255) attCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAAt < 1:3099794/44‑1 (MQ=255) attCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAAt < 1:1586647/44‑1 (MQ=255) attCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAAt < 1:57599/44‑1 (MQ=255) attCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAAt < 1:2179628/44‑1 (MQ=255) agcGGATACGCATACAGACTCCATCCCCCCTCCAGCCCTATCGGGTCCTGAGTGATATACCGCCCctgca < 1:2009461/68‑2 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCg > 1:2323779/1‑42 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCagcagc > 1:1705092/1‑71 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCtgcagc > 1:3842904/1‑71 (MQ=255) aCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc > 1:1264693/1‑71 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCg > 1:971589/1‑71 (MQ=255) ggATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGCCCCTGCAGCgg > 1:3260527/1‑71 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:1823575/35‑1 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTTTTATACCGCCCCTGCTGCGGt < 1:2939456/71‑1 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt < 1:3895595/71‑1 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt < 1:858554/71‑1 (MQ=255) aTACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgggt > 1:2302654/1‑69 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCt < 1:3918603/44‑1 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCt < 1:1972586/44‑1 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCgg > 1:95052/1‑69 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGcc > 1:1034159/1‑42 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTa < 1:1229746/71‑1 (MQ=255) aCTGATAAAAATTCCATCCCCCTTTCAGCCCAATCgg > 1:1441278/1‑37 (MQ=25) aCTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:558483/48‑1 (MQ=255) cTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtga < 1:77414/47‑2 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCgg > 1:1514680/1‑36 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCg < 1:1019278/35‑1 (MQ=255) tGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGAtata > 1:2244817/1‑50 (MQ=255) tGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAc > 1:609916/1‑51 (MQ=255) tGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCTCCCctgc < 1:1437110/60‑1 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGtt > 1:1270654/1‑47 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:230084/1‑48 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:2116853/1‑48 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt > 1:228892/1‑39 (MQ=16) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAg > 1:1450907/1‑41 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:2991903/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:420021/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGt < 1:2367022/70‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGt > 1:1480502/1‑70 (MQ=255) tAAAAATTCCATCCCCCTT‑CAGCCCAATCGGATCCTGAGTGAta > 1:1796954/1‑44 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAAt > 1:1951617/1‑66 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAc > 1:18002/1‑71 (MQ=255) aaaaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtga > 1:1701010/1‑40 (MQ=255) aaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:1885943/1‑70 (MQ=255) aTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGa > 1:1505057/1‑70 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg < 1:2546707/53‑1 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt > 1:3575229/1‑62 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt > 1:462565/1‑62 (MQ=255) acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:1701539/71‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt > 1:1640274/1‑69 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt > 1:3536187/1‑50 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:1234728/1‑70 (MQ=255) | CAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTA > minE/2572639‑2572777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |