| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,752 | G→A | 9.8% | intergenic (+1520/‑259) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,752 | 0 | G | A | 9.8% | 173.1 / 7.8 | 61 | intergenic (+1520/‑259) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (32/23); new base A (4/2); total (36/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCT > minE/2572683‑2572821 | aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCagcagc > 1:1705092/1‑71 (MQ=255)aCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCtgcagc > 1:3842904/1‑71 (MQ=255)aCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc > 1:1264693/1‑71 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCg > 1:971589/1‑71 (MQ=255) ggATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGCCCCTGCAGCgg > 1:3260527/1‑71 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTTTTATACCGCCCCTGCTGCGGt < 1:2939456/71‑1 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt < 1:3895595/71‑1 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt < 1:858554/71‑1 (MQ=255) aTACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgggt > 1:2302654/1‑69 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCgg > 1:95052/1‑69 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTa < 1:1229746/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:420021/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:2991903/71‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGt > 1:1480502/1‑70 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGt < 1:2367022/70‑1 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAAt > 1:1951617/1‑66 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAc > 1:18002/1‑71 (MQ=255) aaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:1885943/1‑70 (MQ=255) aTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGa > 1:1505057/1‑70 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg < 1:2546707/53‑1 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt > 1:462565/1‑62 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt > 1:3575229/1‑62 (MQ=255) acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:1701539/71‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt > 1:1640274/1‑69 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt > 1:3536187/1‑50 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:1234728/1‑70 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:3408042/1‑69 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg > 1:33221/1‑71 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTa > 1:2369483/1‑52 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:2098054/1‑70 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:2448899/1‑69 (MQ=255) cccAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAAt > 1:3901818/1‑44 (MQ=255) cccAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGcc < 1:2415230/71‑1 (MQ=255) cccAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGcc > 1:1589666/1‑71 (MQ=255) aaTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGcg > 1:438408/1‑67 (MQ=255) aaTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt > 1:1836065/1‑70 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt < 1:1580718/70‑1 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt < 1:2982393/70‑1 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt < 1:872306/70‑1 (MQ=255) atccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAGCGataa < 1:654806/44‑1 (MQ=255) aGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGtt < 1:3340648/51‑1 (MQ=255) aGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcat > 1:1845928/1‑71 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcat < 1:2599422/69‑1 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcat < 1:2594785/69‑1 (MQ=255) cTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:2546252/71‑1 (MQ=255) cTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat > 1:2800266/1‑71 (MQ=255) cTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat > 1:2757228/1‑71 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACa > 1:1807583/1‑55 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACa > 1:699314/1‑55 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca > 1:3186258/1‑70 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatc < 1:252641/69‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGCTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa > 1:228955/1‑60 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc < 1:3376186/48‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtacta > 1:2032810/1‑71 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:185563/70‑1 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcatca > 1:2112735/2‑66 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgct < 1:2733995/69‑1 (MQ=255) tataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgctgc < 1:3511058/71‑3 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtactac < 1:1069720/70‑1 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactact > 1:2429657/1‑71 (MQ=255) aCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTActg < 1:2545138/70‑1 (MQ=255) aCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCATGctgctg > 1:3789815/1‑70 (MQ=255) ccccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCg < 1:3033957/71‑1 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCgg > 1:2870024/1‑70 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCgg > 1:2425114/1‑70 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatca < 1:3312446/52‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCa < 1:1001683/71‑1 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt > 1:2934238/1‑44 (MQ=255) ctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGt < 1:3121611/70‑1 (MQ=255) tgcGGTTAATGTTACCGATAATAGTTATAGTACAg < 1:770132/35‑1 (MQ=255) tgcGGTTAATGTTACCGATAATAGTTATAGTACAg < 1:3089169/35‑1 (MQ=255) tgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtacta < 1:3704522/56‑1 (MQ=255) gcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCt > 1:2372701/1‑70 (MQ=255) | ACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCT > minE/2572683‑2572821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |