| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,772 | T→C | 8.1% | intergenic (+1540/‑239) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,772 | 0 | T | C | 8.1% | 203.9 / 7.9 | 74 | intergenic (+1540/‑239) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (38/30); new base C (4/2); total (42/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 | |||||||||||
ACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCTTC > minE/2572704‑2572837 | acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:1701539/71‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt > 1:1640274/1‑69 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:1234728/1‑70 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:3408042/1‑69 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg > 1:33221/1‑71 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:2098054/1‑70 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:2448899/1‑69 (MQ=255) cccAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGcc < 1:2415230/71‑1 (MQ=255) cccAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGcc > 1:1589666/1‑71 (MQ=255) aaTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGcg > 1:438408/1‑67 (MQ=255) aaTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt > 1:1836065/1‑70 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt < 1:2982393/70‑1 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt < 1:1580718/70‑1 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt < 1:872306/70‑1 (MQ=255) aGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGtt < 1:3340648/51‑1 (MQ=255) aGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcat > 1:1845928/1‑71 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcat < 1:2594785/69‑1 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcat < 1:2599422/69‑1 (MQ=255) cTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:2546252/71‑1 (MQ=255) cTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat > 1:2800266/1‑71 (MQ=255) cTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat > 1:2757228/1‑71 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACa > 1:699314/1‑55 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACa > 1:1807583/1‑55 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca > 1:3186258/1‑70 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatc < 1:252641/69‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGCTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa > 1:228955/1‑60 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc < 1:3376186/48‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtacta > 1:2032810/1‑71 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:185563/70‑1 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcatca > 1:2112735/2‑66 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgct < 1:2733995/69‑1 (MQ=255) tataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgctgc < 1:3511058/71‑3 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtactac < 1:1069720/70‑1 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactact > 1:2429657/1‑71 (MQ=255) aCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTActg < 1:2545138/70‑1 (MQ=255) aCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCATGctgctg > 1:3789815/1‑70 (MQ=255) ccccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCg < 1:3033957/71‑1 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCgg > 1:2425114/1‑70 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCgg > 1:2870024/1‑70 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatca < 1:3312446/52‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCa < 1:1001683/71‑1 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt > 1:2934238/1‑44 (MQ=255) ctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGt < 1:3121611/70‑1 (MQ=255) tgcGGTTAATGTTACCGATAATAGTTATAGTACAg < 1:770132/35‑1 (MQ=255) tgcGGTTAATGTTACCGATAATAGTTATAGTACAg < 1:3089169/35‑1 (MQ=255) tgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtacta < 1:3704522/56‑1 (MQ=255) gcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCt > 1:2372701/1‑70 (MQ=255) ggTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtg < 1:1317556/69‑1 (MQ=255) ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgatga < 1:3009111/71‑1 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATtcat > 1:214319/1‑39 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATtc > 1:1359592/1‑37 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATtc > 1:3603738/1‑37 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTActgctg > 1:1943253/1‑55 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGaa < 1:3458468/70‑1 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAAc > 1:3922165/1‑71 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAAc > 1:3355937/1‑71 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAAc > 1:3351774/1‑71 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAAc > 1:2449175/1‑71 (MQ=255) tGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTa > 1:1656148/1‑71 (MQ=255) ttACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTa > 1:1832063/1‑69 (MQ=255) tACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtg < 1:2195102/60‑1 (MQ=255) tACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTAt < 1:3359491/69‑1 (MQ=255) aCCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTActgctg < 1:3285435/49‑1 (MQ=255) aCCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTg < 1:1610509/70‑1 (MQ=255) aCCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGc > 1:3300660/1‑71 (MQ=255) aCCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGc > 1:2264932/1‑71 (MQ=255) ccGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGc < 1:1781048/70‑1 (MQ=255) ccGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCt < 1:1175134/71‑1 (MQ=255) cGATAATAGTTATAGTACAGCCCTGATtcatcatc > 1:1995498/1‑35 (MQ=255) cGATAATAGTTATAGTACAGCCCTGATtcatcatc > 1:2930303/1‑35 (MQ=255) cGATAATAGTTATAGTACAGCCCTGATtcatcatc > 1:3761749/1‑35 (MQ=255) gATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGc > 1:2870997/1‑50 (MQ=255) gATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCttt > 1:1652641/1‑70 (MQ=255) gATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCTTc > 1:3239374/1‑71 (MQ=255) ataataGTTATAGTACAGCCCTGATTCATCATCATACTActgct > 1:2972338/1‑44 (MQ=255) ataataGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTAtt < 1:333820/65‑1 (MQ=255) taataGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtga > 1:2707091/1‑55 (MQ=255) taataGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCtt < 1:11269/68‑1 (MQ=255) | ACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCTTC > minE/2572704‑2572837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |