| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,941 | G→A | 49.0% | intergenic (+1709/‑70) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,941 | 0 | G | A | 49.0% | ‑0.4 / 69.0 | 51 | intergenic (+1709/‑70) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (10/16); new base A (8/17); total (18/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGTAATTTAA > minE/2572873‑2573011 | aTAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGAt < 1:3295118/71‑1 (MQ=255)aTAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGAt < 1:3188118/71‑1 (MQ=255)aTAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGAt > 1:187977/1‑71 (MQ=255)aTAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAAt < 1:619320/71‑1 (MQ=255)aTAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAAt > 1:844976/1‑71 (MQ=255) aTCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGt < 1:1356765/71‑1 (MQ=255) tCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGt > 1:1835705/1‑70 (MQ=255) tCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTa < 1:3258565/71‑1 (MQ=255) aTTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAg < 1:3378349/71‑1 (MQ=255) ggttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGat < 1:2177753/69‑1 (MQ=255) ggttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGat < 1:1533305/69‑1 (MQ=255) ggttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTAGTAAGata < 1:3149796/70‑1 (MQ=255) gttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCATAAGATATc < 1:2446983/71‑1 (MQ=255) ttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTc > 1:259625/1‑60 (MQ=255) tggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAg > 1:967142/1‑71 (MQ=255) gcgcACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAg > 1:3002976/1‑62 (MQ=255) tGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACtat > 1:1929913/1‑70 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATAcc > 1:1452939/1‑71 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGatat < 1:1585914/58‑1 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGatat < 1:1979414/58‑1 (MQ=255) taatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGgaga < 1:1288950/43‑1 (MQ=255) taatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTaa > 1:77168/1‑50 (MQ=255) taatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGa > 1:1230610/1‑70 (MQ=255) taatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGt < 1:703616/48‑1 (MQ=255) aatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACta > 1:1390285/1‑63 (MQ=255) atCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGatat < 1:977178/71‑1 (MQ=255) aGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGAtata < 1:1743912/67‑1 (MQ=255) ttttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAaataat < 1:357918/71‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:2394901/1‑69 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACta > 1:3761166/1‑53 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAataata < 1:3121627/70‑1 (MQ=255) tATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAAtaataa < 1:1447203/70‑1 (MQ=255) aTCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGat < 1:3673999/40‑1 (MQ=255) aTCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGat < 1:2996673/40‑1 (MQ=255) aTCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGat < 1:1323217/40‑1 (MQ=255) cACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACt > 1:1377222/1‑71 (MQ=255) cACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGatat > 1:1882068/1‑40 (MQ=255) aCTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTc < 1:491364/71‑1 (MQ=255) aCTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTc < 1:2833291/71‑1 (MQ=255) tACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc > 1:3801722/1‑71 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACt < 1:3208275/70‑1 (MQ=255) gCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACt < 1:2518800/68‑1 (MQ=255) ataGGGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAAc > 1:2736402/1‑71 (MQ=255) taGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACCTGCAAAcc < 1:925975/71‑1 (MQ=255) gagGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATataaataa < 1:3305894/50‑1 (MQ=255) gagGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATataaataa < 1:1871633/50‑1 (MQ=255) gagGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATataaataa < 1:1579516/50‑1 (MQ=255) gagGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACCTGCAAACCAg < 1:2379440/71‑1 (MQ=255) ggCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGat < 1:599604/40‑1 (MQ=255) cTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACgg < 1:1317361/71‑1 (MQ=255) ttAAGGAAATTCGTAAGATATCAGCCACTATAGCGATATAAATATTAAGACt < 1:599544/52‑1 (MQ=255) ggAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACCTGCAAACCAGACGGTAAtt > 1:1195105/1‑71 (MQ=255) agaTTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGTAATTTa < 1:1640416/71‑1 (MQ=255) gaTTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGTAATTTaa < 1:985088/71‑1 (MQ=255) | ATAAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGTAATTTAA > minE/2572873‑2573011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |