| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,573,339 | T→C | 28.8% | intergenic (+29/‑66) | ysaB → / → glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,573,339 | 0 | T | C | 28.8% | 91.2 / 47.5 | 66 | intergenic (+29/‑66) | ysaB/glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (25/22); new base C (8/11); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAAT > minE/2573271‑2573404 | tGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtata < 1:1281872/70‑1 (MQ=255)tGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtata < 1:586279/70‑1 (MQ=255) aCGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCt < 1:3199686/71‑1 (MQ=255) cgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtat < 1:2002985/64‑1 (MQ=255) gATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcca < 1:3121106/70‑1 (MQ=255) tGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtccatc > 1:3821690/1‑70 (MQ=255) ggCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtccatc > 1:3856533/1‑69 (MQ=255) gCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:1282650/71‑1 (MQ=255) ccATTTTTTGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:3545743/71‑1 (MQ=255) cATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCg > 1:635819/1‑71 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtccat > 1:3222381/1‑64 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCgg < 1:161784/71‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCggg < 1:2590114/71‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCggg < 1:23949/71‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCggg < 1:2362479/71‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCgg > 1:1774731/1‑70 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:2320038/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:1418642/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCggg > 1:3625832/1‑69 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:599776/70‑1 (MQ=255) ttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtat < 1:1988829/70‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:1627846/65‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtc < 1:2236741/52‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtc < 1:2168231/52‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtc < 1:492052/52‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGa < 1:32536/71‑1 (MQ=255) tCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCt < 1:1540638/49‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATc > 1:2935690/1‑71 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATc > 1:3881590/1‑71 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCtt < 1:1466346/48‑1 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATcc > 1:1991702/1‑70 (MQ=255) ccATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTATTGTATATCTTCCATCCAGCGGGTATACTGATCcct < 1:2096044/70‑1 (MQ=255) ccATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCcctt > 1:375782/1‑71 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcc < 1:3199737/50‑1 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGTGTATACTGATCcctt < 1:3880849/70‑1 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttc > 1:1691747/1‑71 (MQ=255) aTGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:2984662/1‑59 (MQ=255) tGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCccttcct < 1:568359/71‑1 (MQ=255) tGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCccttcct > 1:43537/1‑71 (MQ=255) gCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttcctt > 1:3261944/1‑71 (MQ=255) gCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttcctt > 1:1423844/1‑71 (MQ=255) gTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa < 1:2204223/71‑1 (MQ=255) ttAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCggg < 1:2783029/50‑1 (MQ=255) ttAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa > 1:2930578/1‑70 (MQ=255) ttAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa < 1:3241368/70‑1 (MQ=255) ttAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa < 1:2981567/70‑1 (MQ=255) tAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATcc > 1:349569/1‑60 (MQ=255) tAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATcc > 1:745736/1‑60 (MQ=255) tAAGCCATGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATc > 1:6196/1‑59 (MQ=255) aGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATc < 1:3666062/71‑1 (MQ=255) gCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATcc < 1:1479558/71‑1 (MQ=255) ccTGCTTTTTCCCGTTTCGTACTGTATATCTtcca < 1:1497950/35‑1 (MQ=255) ccTGCTTTTTCCCGTTTCGTACTGTATATCTtcca < 1:2617077/35‑1 (MQ=255) tGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCacac > 1:1670708/1‑71 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACg > 1:111509/1‑71 (MQ=255) ttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATc < 1:1653431/70‑1 (MQ=255) tCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATc > 1:3804492/1‑59 (MQ=255) tCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATc > 1:2162060/1‑59 (MQ=255) gTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCAc > 1:3254568/1‑71 (MQ=255) tttCGTACTGTATATCTTCCATCCAGCGGGTATACTGAt > 1:2344292/1‑39 (MQ=255) tttCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCAc > 1:257095/1‑70 (MQ=255) tCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGa < 1:655384/70‑1 (MQ=255) gTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAaat < 1:948701/71‑1 (MQ=255) tACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAaat > 1:3096497/1‑70 (MQ=255) cTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTa > 1:2059966/1‑55 (MQ=255) cTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTa > 1:2920224/1‑55 (MQ=255) cTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAaataa > 1:293267/1‑70 (MQ=255) cTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAaataat < 1:1937055/71‑1 (MQ=255) cTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAaataa > 1:796905/1‑70 (MQ=255) | TGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAAT > minE/2573271‑2573404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |