| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,304 | A→G | 7.5% | H991H (CAT→CAC) ‡ | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,304 | 0 | A | G | 7.5% | 260.7 / 9.8 | 93 | H991H (CAT→CAC) ‡ | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (45/41); new base G (4/3); total (49/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCA > minE/2584238‑2584374 | tGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGAc > 1:2926571/1‑71 (MQ=255)tGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGAc > 1:1688756/1‑71 (MQ=255) ttGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGa > 1:952396/1‑68 (MQ=255) ttGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGAc < 1:1822005/69‑1 (MQ=255) ttGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTg < 1:3066559/71‑1 (MQ=255) gCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa > 1:573182/1‑70 (MQ=255) aaaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCa < 1:772220/71‑1 (MQ=255) aaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAg > 1:472032/1‑71 (MQ=255) aCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc < 1:894614/71‑1 (MQ=255) aCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc > 1:1285875/1‑71 (MQ=255) aCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc > 1:2160972/1‑71 (MQ=255) aCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc < 1:277293/71‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCg < 1:29536/71‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCg < 1:430463/71‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCg < 1:3067802/71‑1 (MQ=255) cccGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATTACTGATAACCAGCgg > 1:1515364/1‑69 (MQ=255) ccGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcag > 1:1901561/1‑71 (MQ=255) ccGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcag > 1:2239470/1‑71 (MQ=255) ccGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcag < 1:2775054/71‑1 (MQ=255) ccGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcag < 1:165949/71‑1 (MQ=255) aTCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:606367/71‑1 (MQ=255) aCGCCGTTACGTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:8647/1‑68 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCg < 1:1381577/58‑1 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCg < 1:1922047/58‑1 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:761617/1‑68 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:1453642/1‑68 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:1972868/1‑68 (MQ=255) ccGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt < 1:2638641/71‑1 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt < 1:389211/70‑1 (MQ=255) gatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaa < 1:3682627/69‑1 (MQ=9) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa > 1:2777140/1‑69 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa > 1:1324176/1‑69 (MQ=255) acctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:548358/1‑68 (MQ=255) acctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:1176241/1‑68 (MQ=255) ctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:2550853/65‑1 (MQ=255) ctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc > 1:3605113/1‑70 (MQ=255) ctaccGCGTTTTGCCCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGcc < 1:3879304/71‑1 (MQ=255) taccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc > 1:1163114/1‑46 (MQ=255) accGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcag > 1:1470166/1‑50 (MQ=255) acagcgTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGag < 1:3860792/68‑1 (MQ=9) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATa < 1:292130/38‑1 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcagca > 1:72767/1‑51 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCag < 1:78835/70‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:2617272/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:825207/1‑68 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:1033604/1‑68 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:458692/1‑48 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg > 1:1638040/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg > 1:798087/1‑71 (MQ=255) ttGCGCGCCAGAACCGGCACCATGCCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGCGGtca > 1:683703/1‑71 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAgg > 1:2755116/1‑59 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAgg > 1:2058887/1‑59 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga > 1:3515077/1‑64 (MQ=255) ctGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtca > 1:3009935/2‑71 (MQ=255) tGCGCGCCAGAACCGGCACCATGAGTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca < 1:449981/70‑1 (MQ=255) gcgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat < 1:2212633/70‑1 (MQ=255) gcgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat < 1:1818497/70‑1 (MQ=255) cgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:2061067/1‑64 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:1064270/71‑1 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg < 1:3726042/70‑1 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg > 1:778072/1‑70 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:995071/59‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:3366805/59‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa < 1:2138555/71‑1 (MQ=255) ccAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg < 1:1522864/61‑1 (MQ=255) ccAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt < 1:2543743/71‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGCGgcagca < 1:2425462/36‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa < 1:1447311/49‑1 (MQ=255) aGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:2330768/1‑47 (MQ=255) aGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg > 1:1199883/1‑71 (MQ=255) aGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg > 1:2286118/1‑71 (MQ=255) gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg > 1:3322800/1‑71 (MQ=255) gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg > 1:806792/1‑71 (MQ=255) gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg > 1:2328731/1‑71 (MQ=255) aaCCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg < 1:1525240/69‑1 (MQ=255) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgagaga > 1:880052/2‑54 (MQ=255) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGAta > 1:243958/2‑66 (MQ=12) aCCGGCACCATGACTGATAACCAGCGGCAGCACGCCga > 1:2378583/1‑38 (MQ=255) cGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt < 1:1801768/71‑1 (MQ=255) cGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt < 1:3420762/71‑1 (MQ=255) ggCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgca < 1:3777631/71‑3 (MQ=9) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:3368213/71‑1 (MQ=255) gCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgca > 1:1112377/1‑68 (MQ=9) gCACCATGACTGATAACCAGCGGCAGCACGCCgaga > 1:1350824/1‑36 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt < 1:2733812/63‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:76557/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:1981888/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:1463295/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:1854631/1‑70 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:1447028/1‑70 (MQ=255) aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAAc > 1:3143752/1‑71 (MQ=255) aCCATGACTGATAACCAGCGGCAGCAAGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:478243/1‑70 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:3305821/71‑1 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:1044603/1‑71 (MQ=255) | TGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCA > minE/2584238‑2584374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |