| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,325 | C→T | 13.6% | V984V (GTG→GTA) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,325 | 0 | C | T | 13.6% | 248.6 / 22.7 | 88 | V984V (GTG→GTA) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (38/38); new base T (5/7); total (43/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
ATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATT > minE/2584260‑2584394 | aTCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:606367/71‑1 (MQ=255) aCGCCGTTACGTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:8647/1‑68 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:1972868/1‑68 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:761617/1‑68 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:1453642/1‑68 (MQ=255) ccGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt < 1:2638641/71‑1 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt < 1:389211/70‑1 (MQ=255) gatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaa < 1:3682627/69‑1 (MQ=9) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa > 1:1324176/1‑69 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa > 1:2777140/1‑69 (MQ=255) acctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:1176241/1‑68 (MQ=255) acctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:548358/1‑68 (MQ=255) ctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:2550853/65‑1 (MQ=255) ctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc > 1:3605113/1‑70 (MQ=255) ctaccGCGTTTTGCCCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGcc < 1:3879304/71‑1 (MQ=255) acagcgTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGag < 1:3860792/68‑1 (MQ=9) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGgcagca > 1:72767/1‑51 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCag < 1:78835/70‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:2617272/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:825207/1‑68 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:1033604/1‑68 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:458692/1‑48 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg > 1:1638040/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg > 1:798087/1‑71 (MQ=255) ttGCGCGCCAGAACCGGCACCATGCCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGCGGtca > 1:683703/1‑71 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAgg > 1:2058887/1‑59 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAgg > 1:2755116/1‑59 (MQ=255) ttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga > 1:3515077/1‑64 (MQ=255) ctGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtca > 1:3009935/2‑71 (MQ=255) tGCGCGCCAGAACCGGCACCATGAGTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtca < 1:449981/70‑1 (MQ=255) gcgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat < 1:1818497/70‑1 (MQ=255) gcgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat < 1:2212633/70‑1 (MQ=255) cgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:2061067/1‑64 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:1064270/71‑1 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg > 1:778072/1‑70 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAg < 1:3726042/70‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:995071/59‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:3366805/59‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa < 1:2138555/71‑1 (MQ=255) ccAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAgg < 1:1522864/61‑1 (MQ=255) ccAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt < 1:2543743/71‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGCGgcagca < 1:2425462/36‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa < 1:1447311/49‑1 (MQ=255) aGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:2330768/1‑47 (MQ=255) aGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg > 1:1199883/1‑71 (MQ=255) aGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg > 1:2286118/1‑71 (MQ=255) gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg > 1:2328731/1‑71 (MQ=255) gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg > 1:806792/1‑71 (MQ=255) gAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg > 1:3322800/1‑71 (MQ=255) aaCCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg < 1:1525240/69‑1 (MQ=255) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgagaga > 1:880052/2‑54 (MQ=255) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGAta > 1:243958/2‑66 (MQ=12) aCCGGCACCATGACTGATAACCAGCGGCAGCACGCCga > 1:2378583/1‑38 (MQ=255) cGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt < 1:1801768/71‑1 (MQ=255) cGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGt < 1:3420762/71‑1 (MQ=255) ggCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgca < 1:3777631/71‑3 (MQ=9) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:3368213/71‑1 (MQ=255) gCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgca > 1:1112377/1‑68 (MQ=9) gCACCATGACTGATAACCAGCGGCAGCACGCCgaga > 1:1350824/1‑36 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt < 1:2733812/63‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:1463295/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:1981888/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa < 1:76557/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:1854631/1‑70 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:1447028/1‑70 (MQ=255) aCCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAAc > 1:3143752/1‑71 (MQ=255) aCCATGACTGATAACCAGCGGCAGCAAGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:478243/1‑70 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:3305821/71‑1 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCa > 1:1044603/1‑71 (MQ=255) ttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca > 1:318409/3‑70 (MQ=2) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATcc < 1:2357308/71‑1 (MQ=255) aCTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATcc < 1:2894015/71‑1 (MQ=255) cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAggac < 1:263185/58‑3 (MQ=9) cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAggac < 1:3151316/58‑3 (MQ=9) cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAggac < 1:3012318/58‑3 (MQ=9) cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCAtac < 1:627934/70‑3 (MQ=9) cTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATcc < 1:35348/70‑1 (MQ=255) tGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTgg < 1:3715236/55‑1 (MQ=255) gATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt < 1:2947039/52‑1 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:2626189/1‑60 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt > 1:1428238/1‑65 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgcg > 1:2795092/1‑70 (MQ=255) tAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc < 1:634815/70‑1 (MQ=255) tAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgcc < 1:264338/71‑1 (MQ=255) aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc < 1:3082112/71‑1 (MQ=255) cAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt < 1:2234995/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc > 1:46695/1‑71 (MQ=255) gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc > 1:2027311/1‑70 (MQ=255) gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg < 1:3706929/71‑1 (MQ=255) cGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg < 1:1936266/70‑1 (MQ=255) gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:2192197/1‑39 (MQ=255) cagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAAc < 1:320007/50‑1 (MQ=255) gcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGAtt < 1:3651375/71‑1 (MQ=255) | ATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATT > minE/2584260‑2584394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |