| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,586,011 | T→C | 8.6% | E422E (GAA→GAG) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,586,011 | 0 | T | C | 8.6% | 192.9 / 8.0 | 70 | E422E (GAA→GAG) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (27/37); new base C (4/2); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
CAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCA > minE/2585941‑2586081 | cAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCt < 1:562269/71‑1 (MQ=255)cAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCt < 1:3597544/71‑1 (MQ=255) tACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTcc > 1:192069/1‑71 (MQ=255) caCCGACCAGCGCACGTTGGATCTGCCCCATCTATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTcc < 1:3480439/70‑1 (MQ=255) cGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCa < 1:3548253/71‑1 (MQ=255) cgcACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa > 1:2228531/1‑68 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGc > 1:726840/1‑59 (MQ=255) gTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc > 1:3465481/1‑70 (MQ=255) gTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc > 1:2087638/1‑70 (MQ=255) gTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCg < 1:2147322/71‑1 (MQ=255) tGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGAc > 1:3050303/1‑71 (MQ=255) tGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGATCGa > 1:737124/1‑70 (MQ=255) ccccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc < 1:1335659/69‑1 (MQ=255) ccccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTcca < 1:1763130/71‑1 (MQ=255) ccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa > 1:1062587/1‑51 (MQ=255) ccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccacc < 1:3363532/71‑1 (MQ=255) cATCGATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTccacca > 1:1872414/1‑71 (MQ=255) aTCGATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTcc < 1:3054131/66‑1 (MQ=255) aTCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac > 1:1533686/1‑71 (MQ=255) aTCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac > 1:20320/1‑71 (MQ=255) aTCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac > 1:3949271/1‑71 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac > 1:1977280/1‑70 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccac > 1:3540943/1‑70 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGac > 1:3065355/1‑47 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:2219701/1‑71 (MQ=255) aTTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTccaccacca > 1:1671793/1‑69 (MQ=255) aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:3886668/1‑70 (MQ=255) aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACg < 1:3494392/71‑1 (MQ=255) aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACg < 1:559040/71‑1 (MQ=255) aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACg < 1:2485590/71‑1 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCg < 1:437012/52‑1 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa < 1:2038118/71‑1 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa < 1:2510934/71‑1 (MQ=255) ttATGCGTCGCTTCCTTCGGTGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa > 1:225237/1‑70 (MQ=255) ttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGAt > 1:1175403/1‑71 (MQ=255) tATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGAt < 1:407216/70‑1 (MQ=255) aTGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg < 1:1426324/70‑1 (MQ=255) aTGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg < 1:3945938/70‑1 (MQ=255) tGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATgg < 1:274622/70‑1 (MQ=255) tGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGc < 1:2282291/71‑1 (MQ=255) gCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCa < 1:2126999/35‑1 (MQ=255) tCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg < 1:689279/47‑1 (MQ=255) tCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccac < 1:184344/55‑1 (MQ=255) cttccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcc < 1:576957/71‑1 (MQ=255) cttccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcc < 1:2360006/71‑1 (MQ=255) tccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac > 1:1285240/1‑71 (MQ=255) tccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac > 1:625026/1‑71 (MQ=255) ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTTATccac < 1:511122/70‑1 (MQ=255) ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:2412808/70‑1 (MQ=255) ttggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAg < 1:2026811/68‑1 (MQ=12) tcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac < 1:1894718/52‑1 (MQ=255) cggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCGCCAGt > 1:1686169/1‑70 (MQ=255) ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTaa > 1:3302608/1‑71 (MQ=255) ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTa < 1:3740850/70‑1 (MQ=255) ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTa < 1:1024032/70‑1 (MQ=255) ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTa < 1:1783259/70‑1 (MQ=255) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc > 1:3736467/1‑71 (MQ=255) ggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca < 1:1971060/64‑1 (MQ=255) ggaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGccc > 1:1364286/3‑70 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa < 1:290303/71‑1 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa < 1:3595756/71‑1 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt > 1:3489502/1‑71 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATc < 1:1975963/71‑1 (MQ=255) gCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGAtg > 1:3295347/1‑70 (MQ=255) ttATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:2878324/1‑71 (MQ=255) tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:3234193/1‑70 (MQ=255) tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:1274685/1‑70 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAccc < 1:1540280/63‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCg < 1:2119080/68‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:3554745/70‑1 (MQ=255) tCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACg < 1:2427728/39‑1 (MQ=255) tCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCa < 1:2884450/70‑1 (MQ=255) ttCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATACACCAGTAACCCTATCGCCAGCa > 1:3488866/1‑71 (MQ=255) | CAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCA > minE/2585941‑2586081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |