Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,586,047 G→T 13.1% I410I (ATC→ATA mdtF ← multidrug transporter, RpoS‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,586,0470GT13.1% 329.6 / 20.5 106I410I (ATC→ATAmdtFmultidrug transporter, RpoS‑dependent
Reads supporting (aligned to +/- strand):  ref base G (35/57);  new base T (8/6);  total (43/63)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAA  >  minE/2585977‑2586116
                                                                      |                                                                     
aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACg                                                                       <  1:2485590/71‑1 (MQ=255)
aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACg                                                                       <  1:3494392/71‑1 (MQ=255)
aTTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACg                                                                       <  1:559040/71‑1 (MQ=255)
 tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa                                                                      <  1:2038118/71‑1 (MQ=255)
 tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa                                                                      <  1:2510934/71‑1 (MQ=255)
  ttATGCGTCGCTTCCTTCGGTGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa                                                                      >  1:225237/1‑70 (MQ=255)
  ttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGAt                                                                     >  1:1175403/1‑71 (MQ=255)
   tATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGAt                                                                     <  1:407216/70‑1 (MQ=255)
    aTGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg                                                                    <  1:3945938/70‑1 (MQ=255)
    aTGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg                                                                    <  1:1426324/70‑1 (MQ=255)
     tGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATgg                                                                   <  1:274622/70‑1 (MQ=255)
     tGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGc                                                                  <  1:2282291/71‑1 (MQ=255)
            cttccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcc                                                           <  1:2360006/71‑1 (MQ=255)
            cttccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcc                                                           <  1:576957/71‑1 (MQ=255)
              tccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac                                                         >  1:625026/1‑71 (MQ=255)
              tccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac                                                         >  1:1285240/1‑71 (MQ=255)
               ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTTATccac                                                         <  1:511122/70‑1 (MQ=255)
               ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac                                                         <  1:2412808/70‑1 (MQ=255)
                  ttggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAg                                                      <  1:2026811/68‑1 (MQ=12)
                    ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTa                                                    <  1:3740850/70‑1 (MQ=255)
                    ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTa                                                    <  1:1024032/70‑1 (MQ=255)
                    ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTa                                                    <  1:1783259/70‑1 (MQ=255)
                     gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc                                                  >  1:3736467/1‑71 (MQ=255)
                         tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa                                              <  1:290303/71‑1 (MQ=255)
                         tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa                                              <  1:3595756/71‑1 (MQ=255)
                          aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt                                             >  1:3489502/1‑71 (MQ=255)
                           gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATc                                            <  1:1975963/71‑1 (MQ=255)
                           gCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGAtg                                            >  1:3295347/1‑70 (MQ=255)
                             ttATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc                                          >  1:2878324/1‑71 (MQ=255)
                              tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc                                          >  1:1274685/1‑70 (MQ=255)
                              tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc                                          >  1:3234193/1‑70 (MQ=255)
                               aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAccc                                                <  1:1540280/63‑1 (MQ=255)
                               aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCg                                           <  1:2119080/68‑1 (MQ=255)
                               aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc                                         <  1:3554745/70‑1 (MQ=255)
                                tCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCa                                        <  1:2884450/70‑1 (MQ=255)
                                  ttCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATACACCAGTAACCCTATCGCCAGCa                                     >  1:3488866/1‑71 (MQ=255)
                                   tCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc                                    >  1:521560/1‑71 (MQ=255)
                                    ccGCAATGACACTCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa                                     <  1:1871226/69‑1 (MQ=255)
                                    ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa                                     <  1:3080823/69‑1 (MQ=255)
                                     cGCAATTACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAcc                                   <  1:2019901/70‑1 (MQ=255)
                                     cGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAcc                                   <  1:59734/70‑1 (MQ=255)
                                     cGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCa                                  <  1:3776617/71‑1 (MQ=255)
                                      gCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt                                             >  1:179953/1‑59 (MQ=255)
                                        aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTaa                                                   >  1:2727636/1‑51 (MQ=255)
                                        aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa                                              <  1:1279990/56‑1 (MQ=255)
                                        aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATcc                               >  1:3364156/1‑71 (MQ=255)
                                        aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATcc                               >  1:253318/1‑71 (MQ=255)
                                          tGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATc                                            >  1:1467828/1‑56 (MQ=255)
                                          tGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATc                                            >  1:3748009/1‑56 (MQ=255)
                                          tGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATccc                              >  1:416224/1‑70 (MQ=255)
                                           gACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc                                    <  1:786780/63‑1 (MQ=255)
                                            acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATGCCGaa                           >  1:2101319/4‑71 (MQ=2)
                                            acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa                           >  1:3790593/4‑71 (MQ=2)
                                            acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa                           >  1:1284789/4‑71 (MQ=2)
                                            acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa                           >  1:1833409/4‑71 (MQ=2)
                                               cGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACa                         >  1:1706856/1‑70 (MQ=2)
                                               cGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt                        <  1:3795494/71‑1 (MQ=255)
                                                gCTCGACGTTCTCCACCACCGCGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCg                             >  1:3353477/1‑65 (MQ=255)
                                                gCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCa                                     <  1:3711481/57‑1 (MQ=255)
                                                gCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCa                                     <  1:1200652/57‑1 (MQ=255)
                                                gCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCa                                     <  1:148099/57‑1 (MQ=255)
                                                gCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGt                                                     <  1:1123023/41‑1 (MQ=255)
                                                gCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa                         >  1:1872219/1‑69 (MQ=255)
                                                gCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa                         >  1:2023796/1‑69 (MQ=255)
                                                 cTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAccc                                                <  1:2353192/45‑1 (MQ=255)
                                                  tCGACGTTCTCCACCACCACGATGGCGTCATccacc                                                        <  1:2801586/36‑1 (MQ=40)
                                                  tCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGt                     >  1:187113/1‑71 (MQ=255)
                                                   cGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGt                     >  1:3416428/1‑70 (MQ=2)
                                                   cGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAAc                          <  1:413724/65‑1 (MQ=255)
                                                   cGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAAc                          <  1:928251/65‑1 (MQ=255)
                                                    gACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGtta                   <  1:85629/71‑3 (MQ=2)
                                                    gACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGtta                   <  1:2084177/71‑3 (MQ=2)
                                                    gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGt                     <  1:2651753/69‑1 (MQ=255)
                                                    gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc                    <  1:2405175/70‑1 (MQ=255)
                                                    gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCa                   <  1:2225889/71‑1 (MQ=255)
                                                      cGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAAc                          >  1:2417964/1‑62 (MQ=255)
                                                      cGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc                 <  1:3045170/71‑1 (MQ=255)
                                                      cGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc                 >  1:417058/1‑71 (MQ=255)
                                                       gTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACg                >  1:1140588/1‑71 (MQ=255)
                                                       gTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACg                <  1:2033255/71‑1 (MQ=255)
                                                       gTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACg                <  1:2210775/71‑1 (MQ=255)
                                                       gTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACg                <  1:2441673/71‑1 (MQ=255)
                                                        ttCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc                 <  1:1396246/69‑1 (MQ=255)
                                                        ttCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgt               >  1:1738287/1‑71 (MQ=255)
                                                         tctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg                                       <  1:3877786/46‑1 (MQ=255)
                                                         tctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgtg              >  1:142341/1‑71 (MQ=255)
                                                              accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt                        <  1:1956328/56‑1 (MQ=255)
                                                              accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt                        <  1:836523/56‑1 (MQ=255)
                                                              accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt         >  1:3757272/1‑71 (MQ=255)
                                                              accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt         >  1:3481322/1‑71 (MQ=255)
                                                              accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt         <  1:916688/71‑1 (MQ=255)
                                                              accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt         >  1:2685255/1‑71 (MQ=255)
                                                                caccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgt               <  1:1421996/63‑1 (MQ=255)
                                                                    acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaa   <  1:1450063/71‑1 (MQ=255)
                                                                    acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaa   <  1:2680586/71‑1 (MQ=255)
                                                                    acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaa   <  1:40117/71‑1 (MQ=255)
                                                                     cGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgtgt             >  1:1537788/1‑60 (MQ=255)
                                                                     cGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaaa  <  1:484202/71‑1 (MQ=255)
                                                                     cGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaaa  <  1:2915695/71‑1 (MQ=255)
                                                                      gATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc                    >  1:124831/1‑52 (MQ=255)
                                                                      |                                                                     
ATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAA  >  minE/2585977‑2586116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: