| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,710 | A→T | 18.3% | F388Y (TTC→TAC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,710 | 0 | A | T | 18.3% | 213.9 / 37.2 | 87 | F388Y (TTC→TAC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (28/43); new base T (10/6); total (38/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGT > minE/311640‑311780 | aaCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga < 1:2189356/71‑1 (MQ=255) tGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc < 1:2971964/70‑1 (MQ=255) cGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAg < 1:3797414/71‑1 (MQ=255) atCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaaaag > 1:3276913/2‑69 (MQ=9) atCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaaaag > 1:2778838/2‑69 (MQ=9) cATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAg > 1:227123/1‑62 (MQ=255) aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaaa > 1:563275/1‑66 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:1824706/71‑1 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:2575103/71‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa < 1:2470315/71‑1 (MQ=255) agcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAagcggc < 1:1648596/63‑1 (MQ=12) agcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat > 1:2319320/3‑70 (MQ=12) agcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat < 1:2299073/69‑2 (MQ=12) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGaa > 1:2471585/1‑53 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc < 1:2742190/71‑1 (MQ=255) gcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGatg > 1:576654/2‑69 (MQ=12) gcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGatg > 1:3815727/2‑69 (MQ=12) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:3497243/1‑71 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACgg > 1:3534739/1‑70 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGc < 1:3191325/51‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:1796073/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:3864917/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2363271/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2124814/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:3006533/70‑1 (MQ=255) cccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCa < 1:690757/69‑1 (MQ=12) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga < 1:899035/46‑1 (MQ=255) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:1565651/69‑1 (MQ=255) cGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCaaac < 1:3652167/71‑2 (MQ=16) gATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:892493/1‑50 (MQ=9) gATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:1247340/1‑50 (MQ=9) gATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:2075371/1‑50 (MQ=9) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt > 1:1668109/1‑71 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt > 1:820374/1‑71 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt < 1:3732859/71‑1 (MQ=255) tGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc > 1:1306873/1‑71 (MQ=255) tGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc > 1:2077061/1‑71 (MQ=255) tGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAagcgg > 1:2130779/1‑54 (MQ=9) tGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAagcgg > 1:3950389/1‑54 (MQ=9) ggCTAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:192406/1‑50 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc > 1:3435692/1‑71 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:2907973/1‑58 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:762918/1‑60 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:3526958/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:2262432/71‑1 (MQ=255) aaGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat < 1:663174/56‑2 (MQ=9) aGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa < 1:772822/42‑1 (MQ=255) gCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:1582607/1‑45 (MQ=255) gCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAg > 1:2540071/1‑70 (MQ=255) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:3730817/1‑55 (MQ=255) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:992243/1‑55 (MQ=255) aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa < 1:3568196/71‑1 (MQ=255) aCCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaa < 1:3435123/71‑1 (MQ=12) ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:1463810/46‑1 (MQ=255) aTCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGa > 1:3509140/1‑64 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggc < 1:170205/40‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc < 1:2946875/52‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc < 1:865345/52‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATac < 1:1866087/70‑1 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc < 1:1104027/58‑1 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATacca < 1:742993/70‑1 (MQ=255) cGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:3615318/53‑1 (MQ=255) cGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:832086/53‑1 (MQ=255) cGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:2476207/53‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcg < 1:2982814/37‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGGCGGCAAAGGTCCCGAGCAATACCACCg > 1:3583996/1‑71 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg < 1:2780201/71‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg < 1:3451732/71‑1 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:1878588/71‑1 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:2634466/71‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:899232/70‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg > 1:1436073/1‑70 (MQ=255) cATTGTTTGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa < 1:716698/71‑1 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa > 1:313318/1‑71 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa > 1:41513/1‑71 (MQ=255) aTTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAc > 1:3030104/1‑71 (MQ=255) tGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGa > 1:774512/1‑53 (MQ=255) gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACgg > 1:537101/1‑70 (MQ=255) ttAGCGTGTTTATCGAGAAGCCAAAGGCGGCTAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGc < 1:1383837/70‑1 (MQ=255) ttAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc < 1:1533185/48‑1 (MQ=255) tAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa > 1:1543152/1‑54 (MQ=255) aGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATaccac > 1:1478909/1‑59 (MQ=255) cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATgg < 1:2288479/71‑1 (MQ=255) cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATgg < 1:3819116/71‑1 (MQ=255) cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATgg < 1:1681432/71‑1 (MQ=255) gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGt > 1:1594944/1‑71 (MQ=255) tgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:621452/35‑1 (MQ=255) tATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGa < 1:2700496/41‑1 (MQ=255) aTCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGaa > 1:300351/1‑70 (MQ=255) tCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAAt < 1:2453210/70‑1 (MQ=255) agaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCGATGGTCGGAATCAAc > 1:1445490/1‑71 (MQ=255) agaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAAc < 1:3123425/71‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCa < 1:573145/68‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCa < 1:811350/68‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg > 1:2081468/1‑71 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg < 1:3945945/71‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg < 1:2862872/71‑1 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa > 1:1138492/1‑38 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGt > 1:464883/1‑71 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGt > 1:460268/1‑71 (MQ=255) | AACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGT > minE/311640‑311780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |