| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,586,067 | A→G | 11.1% | L404L (TTA→CTA) ‡ | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,586,067 | 0 | A | G | 11.1% | 317.7 / 24.8 | 117 | L404L (TTA→CTA) ‡ | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (43/61); new base G (8/5); total (51/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
GGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGATCGCA > minE/2585997‑2586137 | ggcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTaa > 1:3302608/1‑71 (MQ=255) ggaGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGccc > 1:1364286/3‑70 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa < 1:290303/71‑1 (MQ=255) tAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa < 1:3595756/71‑1 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt > 1:3489502/1‑71 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATc < 1:1975963/71‑1 (MQ=255) gCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGAtg > 1:3295347/1‑70 (MQ=255) ttATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:2878324/1‑71 (MQ=255) tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:1274685/1‑70 (MQ=255) tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGc > 1:3234193/1‑70 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:3554745/70‑1 (MQ=255) ttCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATACACCAGTAACCCTATCGCCAGCa > 1:3488866/1‑71 (MQ=255) tCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:521560/1‑71 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa < 1:3080823/69‑1 (MQ=255) cGCAATTACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAcc < 1:2019901/70‑1 (MQ=255) cGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAcc < 1:59734/70‑1 (MQ=255) gCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt > 1:179953/1‑59 (MQ=255) aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTaa > 1:2727636/1‑51 (MQ=255) aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATcc > 1:253318/1‑71 (MQ=255) aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATcc > 1:3364156/1‑71 (MQ=255) tGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATc > 1:1467828/1‑56 (MQ=255) tGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATc > 1:3748009/1‑56 (MQ=255) gACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc < 1:786780/63‑1 (MQ=255) acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATGCCGaa > 1:2101319/4‑71 (MQ=2) acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa > 1:1833409/4‑71 (MQ=2) acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa > 1:3790593/4‑71 (MQ=2) acgcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa > 1:1284789/4‑71 (MQ=2) cGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACa > 1:1706856/1‑70 (MQ=2) cGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt < 1:3795494/71‑1 (MQ=255) gCTCGACGTTCTCCACCACCGCGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCg > 1:3353477/1‑65 (MQ=255) gCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCa < 1:3711481/57‑1 (MQ=255) gCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCa < 1:148099/57‑1 (MQ=255) gCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCa < 1:1200652/57‑1 (MQ=255) gCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa > 1:944151/1‑69 (MQ=255) gCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa > 1:1872219/1‑69 (MQ=255) tCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGt > 1:187113/1‑71 (MQ=255) cGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAAc < 1:928251/65‑1 (MQ=255) cGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAAc < 1:413724/65‑1 (MQ=255) gACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGtta < 1:2084177/71‑3 (MQ=2) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGt < 1:2651753/69‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:2405175/70‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCa < 1:2225889/71‑1 (MQ=255) aCGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAg > 1:2130414/1‑67 (MQ=255) cGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAAc > 1:2417964/1‑62 (MQ=255) cGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc > 1:417058/1‑71 (MQ=255) cGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc < 1:3045170/71‑1 (MQ=255) gTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACg > 1:1140588/1‑71 (MQ=255) gTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACg < 1:2210775/71‑1 (MQ=255) gTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACg < 1:2033255/71‑1 (MQ=255) gTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACg < 1:2441673/71‑1 (MQ=255) ttCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAAc < 1:1396246/69‑1 (MQ=255) ttCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgt > 1:1738287/1‑71 (MQ=255) tctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg < 1:3877786/46‑1 (MQ=255) tctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgtg > 1:142341/1‑71 (MQ=255) accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt < 1:836523/56‑1 (MQ=255) accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt < 1:1956328/56‑1 (MQ=255) accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt < 1:916688/71‑1 (MQ=255) accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt > 1:3757272/1‑71 (MQ=255) accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt > 1:3481322/1‑71 (MQ=255) caccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgt < 1:1421996/63‑1 (MQ=255) cacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGa < 1:2792971/71‑1 (MQ=255) cacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGa < 1:3618446/71‑1 (MQ=255) acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaa < 1:40117/71‑1 (MQ=255) acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaa < 1:2680586/71‑1 (MQ=255) cGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgtgt > 1:1537788/1‑60 (MQ=255) cGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaaa < 1:2915695/71‑1 (MQ=255) cGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaaa < 1:484202/71‑1 (MQ=255) gATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc > 1:124831/1‑52 (MQ=255) aTGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAacc < 1:3423260/71‑1 (MQ=255) aTGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAacc < 1:3099023/71‑1 (MQ=255) tGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAaccg < 1:3864657/71‑1 (MQ=255) gCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAaccga < 1:1753339/70‑1 (MQ=255) gTCATCCACCAGTAACCCTATCGCCAGCACCATCTCGAACATAGTCAAc < 1:3740599/49‑1 (MQ=255) tCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATg < 1:2577487/57‑1 (MQ=255) tCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgc < 1:3925589/71‑1 (MQ=255) cATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgt > 1:3763548/1‑49 (MQ=255) cATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgcc > 1:2434099/1‑71 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt > 1:178616/1‑39 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAac > 1:212543/1‑62 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgcc > 1:1729638/1‑70 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgcc < 1:1155688/70‑1 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgcc > 1:1610756/1‑70 (MQ=255) caccaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGAc < 1:923957/70‑1 (MQ=255) caccaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGAc < 1:1942710/70‑1 (MQ=255) accaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGAccgc < 1:625019/65‑1 (MQ=255) accaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACaa < 1:673907/71‑1 (MQ=255) ccaGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:2271318/38‑1 (MQ=255) ccaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGAc > 1:2024760/1‑68 (MQ=255) ccaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAg < 1:694486/71‑1 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGa < 1:738470/47‑1 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaaa > 1:522119/1‑55 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGaaa > 1:417796/1‑55 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGa < 1:3929530/71‑1 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGa < 1:852538/71‑1 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGa < 1:2857097/71‑1 (MQ=255) caGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGa < 1:2619898/71‑1 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTg > 1:664354/1‑45 (MQ=255) aGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTg > 1:146125/1‑45 (MQ=255) aCCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGATCGc < 1:356836/70‑1 (MQ=255) aCCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGATCGCa < 1:3838294/71‑1 (MQ=255) | GGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGACAAGATCGCA > minE/2585997‑2586137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |