| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,587,282 | T→C | 10.9% | intergenic (‑6/+19) | mdtF ← / ← mdtE | multidrug transporter, RpoS‑dependent/multidrug resistance efflux transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,587,282 | 0 | T | C | 10.9% | 119.8 / 6.3 | 46 | intergenic (‑6/+19) | mdtF/mdtE | multidrug transporter, RpoS‑dependent/multidrug resistance efflux transporter |
| Reads supporting (aligned to +/- strand): ref base T (21/20); new base C (3/2); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
GCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAATTGCTCG > minE/2587212‑2587351 | gCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGt > 1:1146847/1‑71 (MQ=255) atcatAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCtt < 1:599994/71‑1 (MQ=255) ataataATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAg < 1:1925123/71‑1 (MQ=255) aataatGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGcc > 1:2504207/1‑71 (MQ=255) ataatGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCt > 1:1863013/1‑71 (MQ=255) atGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGc < 1:2116179/70‑1 (MQ=255) tGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCaa < 1:3002966/71‑1 (MQ=255) aaGTACCCAGGCAAAAACCGGGCCATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGt > 1:718176/1‑70 (MQ=255) cccAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGt < 1:684563/71‑1 (MQ=255) ccAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGt < 1:75064/70‑1 (MQ=255) cAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGttt > 1:3605232/1‑71 (MQ=255) gCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGa > 1:3018641/1‑71 (MQ=255) aaaaaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTc < 1:1840941/67‑1 (MQ=255) aaaaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGttt < 1:2577515/65‑1 (MQ=255) aaaaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCg < 1:1441160/67‑1 (MQ=255) aaaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTccc > 1:608129/1‑40 (MQ=255) aaaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGc < 1:3449815/47‑1 (MQ=255) aaaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCg < 1:3626268/71‑1 (MQ=255) aaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCgg > 1:757200/1‑71 (MQ=255) ccGGGCGATCAATAAAATAGTTAGCCATGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGt > 1:3677879/1‑70 (MQ=255) gggCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAAc > 1:3254242/1‑49 (MQ=255) gggCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCt > 1:3626221/1‑71 (MQ=255) gggCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCt > 1:2721640/1‑71 (MQ=255) ggCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTg > 1:45585/1‑71 (MQ=255) gCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCg > 1:1684710/1‑64 (MQ=255) gCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTgg < 1:55577/71‑1 (MQ=255) tCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGAtt < 1:2492860/58‑1 (MQ=255) tCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGtt > 1:3419888/1‑71 (MQ=255) aaTAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGtttt < 1:314787/71‑1 (MQ=255) aaTAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGttt < 1:1496404/70‑1 (MQ=255) aaaaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGc > 1:200330/1‑59 (MQ=255) aaaaTAGTTAGCCATGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCct > 1:3443980/1‑71 (MQ=255) aaaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCctg > 1:1831889/1‑71 (MQ=255) aaaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCct < 1:2585674/70‑1 (MQ=255) aaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTa > 1:829744/1‑40 (MQ=255) aaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTa > 1:1714123/1‑40 (MQ=255) tAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCctggct < 1:897587/71‑1 (MQ=255) aGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTc > 1:808967/1‑64 (MQ=255) aTGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAAt > 1:1886671/1‑71 (MQ=255) aTGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAAt > 1:2136880/1‑71 (MQ=255) tGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTc < 1:1459178/40‑1 (MQ=255) tGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTc < 1:3291854/40‑1 (MQ=255) aaaGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTcc > 1:2178674/1‑54 (MQ=255) aaaGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAATTGc > 1:3850437/1‑71 (MQ=255) aaaGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAATTGc < 1:3350851/71‑1 (MQ=255) gTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGaa < 1:3214516/63‑1 (MQ=255) gTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAATTGCTCg < 1:2140962/71‑1 (MQ=255) tCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTggagga < 1:2299016/61‑1 (MQ=255) | GCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAATTGCTCG > minE/2587212‑2587351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |