| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,592,861 | C→G | 5.1% | M49I (ATG→ATC) ‡ | yhiF ← | predicted DNA‑binding ranscriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,592,861 | 0 | C | G | 5.1% | 301.9 / 2.9 | 96 | M49I (ATG→ATC) ‡ | yhiF | predicted DNA‑binding ranscriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (42/49); new base G (2/3); total (44/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGTATAT > minE/2592792‑2592931 | gaAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCa > 1:43629/1‑71 (MQ=255)gaAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCa > 1:3216908/1‑71 (MQ=255)gaAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCa > 1:3324637/1‑71 (MQ=255) aaaTAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCAt > 1:3761343/1‑70 (MQ=255) aaaTAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCAt > 1:303299/1‑70 (MQ=255) aaaTAATGACATGAACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCAt > 1:1815246/1‑70 (MQ=255) tGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTa < 1:3236935/71‑1 (MQ=255) tGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTa < 1:807356/71‑1 (MQ=255) aCATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTAt > 1:3611806/1‑70 (MQ=255) aTGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaa > 1:28275/1‑71 (MQ=255) aTGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaa > 1:2289690/1‑71 (MQ=255) ggACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaat > 1:3344293/1‑70 (MQ=255) gACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataa > 1:1926364/1‑71 (MQ=255) gACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaata < 1:1608663/70‑1 (MQ=255) gACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaata < 1:2262374/70‑1 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat > 1:1278632/1‑71 (MQ=255) aCAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaataat < 1:38171/71‑1 (MQ=255) cAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATg < 1:1672743/71‑1 (MQ=255) gTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg < 1:2557239/71‑1 (MQ=255) gTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg < 1:1647664/71‑1 (MQ=255) ttttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:806003/71‑1 (MQ=255) ttttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:2344513/71‑1 (MQ=255) tttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTGATGTATCAATAATGACg > 1:2738810/1‑69 (MQ=255) tttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg > 1:806147/1‑69 (MQ=255) tttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg > 1:1979347/1‑69 (MQ=255) tttAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGa < 1:673487/70‑1 (MQ=255) tAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAAt > 1:215471/1‑52 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCaat < 1:2337212/57‑1 (MQ=255) tCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCa < 1:2320471/70‑1 (MQ=255) ttCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATg < 1:3686424/58‑1 (MQ=255) tCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCt > 1:117183/1‑70 (MQ=255) tCAATTTGAGTGAGAAAATTAGAATGAAATACATTAATGAATAATGTAtt > 1:424483/1‑49 (MQ=25) tCAATTTGAGTGAGAAAATTAGAATGAAATACATTAATGAATAATGTAtt > 1:2445290/1‑49 (MQ=25) cAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGa > 1:1848234/1‑71 (MQ=255) aaTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGa < 1:2599165/70‑1 (MQ=255) aaTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGAt > 1:2857707/1‑71 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTa < 1:2002778/45‑1 (MQ=255) aTTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATc < 1:514463/47‑1 (MQ=255) tgagtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCaa > 1:262060/1‑71 (MQ=255) tgagtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCaa > 1:1615748/1‑71 (MQ=255) gagtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCaa < 1:2563118/70‑1 (MQ=255) agtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACa < 1:470235/71‑1 (MQ=255) gtgagAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACAt < 1:264206/71‑1 (MQ=255) tgagAAAATTAGAATGAAATACATTATTCATTAATGTATCaat < 1:2032589/43‑1 (MQ=255) gagaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTc < 1:3951163/58‑1 (MQ=255) agaAAATTAGAATGATATACATTATTCATTAATGTATCtataa < 1:2653233/43‑1 (MQ=255) agaAAATTAGAATGAAATAGATTATTCATTAATGTATCAATAATGACGAAGGCAt < 1:3096229/55‑1 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCAt < 1:2485101/55‑1 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTa > 1:3281355/1‑70 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc > 1:1588316/1‑71 (MQ=255) agaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTCATg > 1:2983063/1‑62 (MQ=255) gaAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACg < 1:1038073/71‑1 (MQ=255) aaaaTTAGAATGAAATACATTATTCATTGATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGt < 1:668691/71‑1 (MQ=255) aaaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACg < 1:154280/46‑1 (MQ=255) aaaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAgg < 1:3738218/50‑1 (MQ=255) aaaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAgg < 1:2797535/50‑1 (MQ=255) aaaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAgg < 1:3455950/50‑1 (MQ=255) aaaTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTc < 1:1660064/71‑1 (MQ=255) aTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAgg < 1:38115/47‑1 (MQ=255) aTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCg > 1:3191171/1‑70 (MQ=255) aTTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGa > 1:517765/1‑71 (MQ=255) tAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACg > 1:1449385/1‑65 (MQ=255) tAGAATGAAATACATTAGTCATTAATGTATCaataat > 1:19590/1‑37 (MQ=255) aaTGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATctct > 1:1201528/1‑71 (MQ=255) aTGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATctct < 1:189994/70‑1 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttc > 1:3774142/1‑71 (MQ=255) tGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttc > 1:3796929/1‑71 (MQ=255) aaaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttct < 1:2930656/70‑1 (MQ=255) aaaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttct < 1:2263095/70‑1 (MQ=255) aaaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttct < 1:27849/70‑1 (MQ=255) aaaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttct > 1:2357981/1‑70 (MQ=255) gaTACATTAATGAATAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTAc < 1:42363/55‑1 (MQ=37) gaTACATTAATGAATAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtc < 1:1835336/62‑1 (MQ=37) gaTACATTAATGAATAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGAtc < 1:128008/62‑1 (MQ=37) aaTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCttcttc > 1:2046257/1‑71 (MQ=255) aTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCttcttc > 1:2136852/1‑70 (MQ=255) aTACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCg > 1:2415162/1‑71 (MQ=255) tACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCg < 1:2289758/58‑1 (MQ=255) tACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttct < 1:1163663/67‑1 (MQ=255) tACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGt < 1:1396905/71‑1 (MQ=255) tACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGt < 1:2416219/71‑1 (MQ=255) tACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGt > 1:2208794/1‑71 (MQ=255) aCATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCg < 1:1518898/57‑1 (MQ=255) cATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTc < 1:1766024/71‑1 (MQ=255) attattCATTAATGTATCAATAATGACGAAGGCATTCTGa > 1:3543313/1‑40 (MQ=255) ttattCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTg < 1:2616465/71‑1 (MQ=255) ttattCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTg > 1:1679885/1‑71 (MQ=255) tattCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCtcttct > 1:2955944/1‑62 (MQ=255) tattCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTg > 1:63775/1‑70 (MQ=255) attCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTc > 1:925284/1‑51 (MQ=255) attCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCttcttc < 1:2205570/63‑1 (MQ=255) attCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCttcttc < 1:3510403/63‑1 (MQ=255) attCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGt < 1:2317219/70‑1 (MQ=255) attCATTAATGTATCAATAATGAAGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGta > 1:2249072/1‑71 (MQ=255) ttCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGtat < 1:41333/71‑1 (MQ=255) ttCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGtat < 1:1338920/71‑1 (MQ=255) cATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGTatat > 1:2117104/1‑71 (MQ=255) | GAAAATAATGACATGGACAGGTTTTAATCGTTCAATTTGAGTGAGAAAATTAGAATGAAATACATTATTCATTAATGTATCAATAATGACGAAGGCATTCTGATGCAACATTACGTCGATCTCTTCTTCGTTCTGTATAT > minE/2592792‑2592931 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |