BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,595,248	A→G	23.2%	R16R (CGT→CGC)	arsC ←	arsenate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,595,248	0	A	G	23.2%	205.9 / 63.5	112	R16R (CGT→CGC)	arsC	arsenate reductase
Reads supporting (aligned to +/- strand): ref base A (48/38); new base G (12/14); total (60/52)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 120 total aligned reads displayed.

TGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTG  >  minE/2595179‑2595317

tGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAc                                                                      >  1:3429468/1‑71 (MQ=255)

  gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                                                    <  1:3148513/71‑1 (MQ=255)

  gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                                                    <  1:2682631/71‑1 (MQ=255)

  gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGATGTTGCGGATCATCTCCAGCGTATTAcgc                                                                    <  1:572767/71‑1 (MQ=255)

   cGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgcg                                                                   <  1:3269313/71‑1 (MQ=255)

      aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTa                                                                       >  1:833701/1‑64 (MQ=255)

      aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACg                                                                <  1:879926/71‑1 (MQ=255)

      aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACg                                                                >  1:3305580/1‑71 (MQ=255)

      aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACg                                                                >  1:3149622/1‑71 (MQ=255)

        tttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTg                                                              >  1:2816590/1‑71 (MQ=255)

        tttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTg                                                              >  1:3519560/1‑71 (MQ=255)

        tttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTg                                                              >  1:2440572/1‑71 (MQ=255)

         ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGc                                                             >  1:1903062/1‑71 (MQ=255)

          tcgaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGc                                                             >  1:2230615/4‑70 (MQ=255)

          tcgaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGc                                                             <  1:210215/67‑1 (MQ=255)

          tCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGc                                                             <  1:1160763/70‑1 (MQ=255)

           ccAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                                            <  1:1578844/70‑1 (MQ=255)

           ccAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                                            <  1:3564941/70‑1 (MQ=255)

            gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGccac                                                          <  1:1365175/70‑3 (MQ=255)

            cAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGc                                                          <  1:545457/71‑1 (MQ=255)

             aGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCcaca                                                         <  1:3064865/71‑1 (MQ=255)

             aGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTa                                                                       >  1:3889924/1‑57 (MQ=255)

             aGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                                           >  1:2546880/1‑69 (MQ=255)

               aTAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAgg                                                       >  1:31737/1‑71 (MQ=255)

                tAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGc                                                      <  1:2711013/71‑1 (MQ=255)

                tAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGc                                                      >  1:2706900/1‑71 (MQ=255)

                 aaTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCa                                                         >  1:2572014/1‑67 (MQ=255)

                  aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGGGACGTGCCGCAGGcc                                                     <  1:1000847/70‑1 (MQ=255)

                  aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                                           <  1:3754353/64‑1 (MQ=255)

                   tGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCgg                                                   >  1:696829/1‑71 (MQ=255)

                   tGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCgg                                                   >  1:693838/1‑71 (MQ=255)

                      ataataGTCGGTTCTGTGCCGCTGTTGCTGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt                                                <  1:1677810/71‑1 (MQ=255)

                      ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt                                                <  1:1867950/71‑1 (MQ=255)

                      ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt                                                <  1:1060286/71‑1 (MQ=255)

                      ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt                                                <  1:817088/71‑1 (MQ=255)

                      ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGt                                                 >  1:3857410/1‑70 (MQ=255)

                       taataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                                                  >  1:2391373/1‑52 (MQ=255)

                       taataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                                                  >  1:3084155/1‑52 (MQ=255)

                        aataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                                              >  1:1556364/1‑71 (MQ=255)

                         attgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgt                                              <  1:2587251/67‑1 (MQ=255)

                         ataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgtg                                             <  1:3801900/71‑1 (MQ=255)

                         ataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgtg                                             <  1:676431/71‑1 (MQ=255)

                          ttgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGa                                            <  1:672465/69‑1 (MQ=255)

                          taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTa                                                                       >  1:3483684/1‑44 (MQ=255)

                          taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGc                                                          >  1:2458016/1‑57 (MQ=255)

                             tCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATa                                          <  1:1639213/70‑1 (MQ=255)

                              cGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                                         >  1:1503227/1‑70 (MQ=255)

                               ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaaa                                        <  1:3376541/70‑1 (MQ=255)

                               ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAAt                                       >  1:166687/1‑71 (MQ=255)

                               ggTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGa                                                                  >  1:1047466/1‑44 (MQ=255)

                               ggTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGAt                                       >  1:3355037/1‑71 (MQ=255)

                                   cTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGt                                    >  1:322149/1‑70 (MQ=255)

                                    tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCggg                                                  <  1:1398041/55‑1 (MQ=255)

                                      tgCCGCTGTTGCGTATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgtg                                             <  1:1139418/58‑1 (MQ=255)

                                            tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCt                           >  1:363305/1‑70 (MQ=255)

                                              ttGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTT‑CTCat                        <  1:2393005/70‑1 (MQ=255)

                                               tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCt                           >  1:1855616/1‑67 (MQ=255)

                                               tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCt                           >  1:2881427/1‑67 (MQ=255)

                                               tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCata                       >  1:497094/1‑71 (MQ=255)

                                               tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCata                       >  1:2731512/1‑71 (MQ=255)

                                               tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCata                       >  1:1013104/1‑71 (MQ=255)

                                               tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCata                       >  1:2579112/1‑71 (MQ=255)

                                                 cGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                                         <  1:1213714/51‑1 (MQ=255)

                                                  ggATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTaa                                  <  1:2820987/57‑1 (MQ=255)

                                                  ggATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCat                        <  1:3618691/67‑1 (MQ=255)

                                                   gATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAg                   <  1:2906178/71‑1 (MQ=255)

                                                    atcatcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAg                   >  1:1012718/1‑70 (MQ=255)

                                                    atcatcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAg                   >  1:2867014/1‑70 (MQ=255)

                                                    atcatcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGt                  <  1:3667590/71‑1 (MQ=255)

                                                    atcatcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGt                  <  1:1984321/71‑1 (MQ=255)

                                                    agcatcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATc                     <  1:471410/66‑1 (MQ=255)

                                                    agcatcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAg                   >  1:3036614/3‑70 (MQ=255)

                                                    agcatcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGt                  <  1:2259287/69‑1 (MQ=255)

                                                      catcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTa                 >  1:2241728/1‑70 (MQ=255)

                                                       atcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGt                               >  1:3055752/1‑55 (MQ=255)

                                                       atcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtc               >  1:3917528/1‑71 (MQ=255)

                                                       atcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtc               >  1:2552801/1‑71 (MQ=255)

                                                        tctcCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGtt                                                <  1:1749654/37‑1 (MQ=16)

                                                        tctcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtct              >  1:2415416/1‑71 (MQ=255)

                                                        tctcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTAATATCAGTAtct              >  1:3822883/1‑71 (MQ=255)

                                                        tctcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGT‑ATGTTGCTCATATCAGTAtct              >  1:2076000/1‑70 (MQ=255)

                                                          tcCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGAt                                       <  1:201980/44‑1 (MQ=255)

                                                          tcCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGAt                                       <  1:1714810/44‑1 (MQ=255)

                                                          tcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCa            >  1:1889602/1‑71 (MQ=255)

                                                          tcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCa            >  1:756080/1‑71 (MQ=255)

                                                            cAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTAtctaa            <  1:3380061/69‑3 (MQ=255)

                                                            cAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATCGTAATGTTGCTCATATCAGTAtct              <  1:3723317/67‑1 (MQ=255)

                                                            cAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtctc             <  1:1560621/68‑1 (MQ=255)

                                                            cAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtctc             <  1:2656478/68‑1 (MQ=255)

                                                            cAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCa            <  1:3209063/69‑1 (MQ=255)

                                                              gCGTGTTGCGTGAGGTGCTACAGGCCGGGTTGTGATAGATGGTAATGTTGATCATATCAGTATCTAATTa         >  1:3424739/1‑70 (MQ=255)

                                                              gCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTAtctaa            >  1:1868750/1‑65 (MQ=255)

                                                              gCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTAtctaa            >  1:307983/1‑65 (MQ=255)

                                                              gCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTa         >  1:2326959/1‑70 (MQ=255)

                                                              gCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTa                 >  1:2126527/1‑62 (MQ=255)

                                                              gCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTa         >  1:1590420/1‑70 (MQ=255)

                                                               cGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACa       <  1:3436311/71‑1 (MQ=255)

                                                                     aCGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTa                                   >  1:2242104/1‑37 (MQ=255)

                                                                     aCGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTg  >  1:3637173/1‑70 (MQ=255)

                                                                     aCGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTg  <  1:754711/70‑1 (MQ=255)

TGGCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTG  >  minE/2595179‑2595317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`