BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,595,251	C→T	23.9%	S15S (TCG→TCA)	arsC ←	arsenate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,595,251	0	C	T	23.9%	204.5 / 60.5	109	S15S (TCG→TCA)	arsC	arsenate reductase
Reads supporting (aligned to +/- strand): ref base C (48/34); new base T (12/14); total (60/49)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 118 total aligned reads displayed.

GCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAG  >  minE/2595181‑2595321

gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                                                        <  1:2682631/71‑1 (MQ=255)

gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgc                                                                        <  1:3148513/71‑1 (MQ=255)

gCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGATGTTGCGGATCATCTCCAGCGTATTAcgc                                                                        <  1:572767/71‑1 (MQ=255)

 cGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTAcgcg                                                                       <  1:3269313/71‑1 (MQ=255)

    aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACg                                                                    <  1:879926/71‑1 (MQ=255)

    aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACg                                                                    >  1:3305580/1‑71 (MQ=255)

    aGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACg                                                                    >  1:3149622/1‑71 (MQ=255)

      tttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTg                                                                  >  1:2440572/1‑71 (MQ=255)

      tttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTg                                                                  >  1:3519560/1‑71 (MQ=255)

      tttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTg                                                                  >  1:2816590/1‑71 (MQ=255)

       ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGc                                                                 >  1:1903062/1‑71 (MQ=255)

       ttCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGc                                                                 >  1:1793698/1‑71 (MQ=255)

        tcgaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGc                                                                 >  1:2230615/4‑70 (MQ=255)

        tcgaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGc                                                                 <  1:210215/67‑1 (MQ=255)

         ccAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                                                <  1:1578844/70‑1 (MQ=255)

         ccAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGcc                                                                <  1:3564941/70‑1 (MQ=255)

          gaGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGccac                                                              <  1:1365175/70‑3 (MQ=255)

          cAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGc                                                              <  1:3867493/71‑1 (MQ=255)

          cAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGc                                                              <  1:545457/71‑1 (MQ=255)

           aGATAATTAATTATTGTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCcaca                                                             <  1:3064865/71‑1 (MQ=255)

           aGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                                               >  1:325666/1‑69 (MQ=255)

           aGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                                               >  1:2546880/1‑69 (MQ=255)

             aTAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAgg                                                           >  1:31737/1‑71 (MQ=255)

              tAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGc                                                          <  1:2711013/71‑1 (MQ=255)

                aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGGGACGTGCCGCAGGcc                                                         <  1:1000847/70‑1 (MQ=255)

                aTGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCg                                                               <  1:3754353/64‑1 (MQ=255)

                 tGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCgg                                                       >  1:693838/1‑71 (MQ=255)

                    ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt                                                    <  1:817088/71‑1 (MQ=255)

                    ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGtt                                                    <  1:1060286/71‑1 (MQ=255)

                    ataataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGt                                                     >  1:3857410/1‑70 (MQ=255)

                     taataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                                                      >  1:3084155/1‑52 (MQ=255)

                     taataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGa                                                                      >  1:2391373/1‑52 (MQ=255)

                      aataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgt                                                  >  1:1556364/1‑71 (MQ=255)

                       attgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgt                                                  <  1:2587251/67‑1 (MQ=255)

                       ataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgtg                                                 <  1:57239/71‑1 (MQ=255)

                       ataGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgtg                                                 <  1:676431/71‑1 (MQ=255)

                        ttgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGa                                                <  1:672465/69‑1 (MQ=255)

                        taGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGc                                                              >  1:2458016/1‑57 (MQ=255)

                         tgTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgtg                                                 >  1:3721245/2‑69 (MQ=255)

                          gTCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTtgtg                                                 >  1:217582/1‑68 (MQ=255)

                           tCGGTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATa                                              <  1:1639213/70‑1 (MQ=255)

                            cGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                                             >  1:1503227/1‑70 (MQ=255)

                             ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaaa                                            <  1:3376541/70‑1 (MQ=255)

                             ggTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAAt                                           >  1:166687/1‑71 (MQ=255)

                             ggTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGa                                                                      >  1:1047466/1‑44 (MQ=255)

                             ggTTCGTTGCCGTTGTTATGAAGCATCTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGAt                                           >  1:3355037/1‑71 (MQ=255)

                                 cTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGt                                        >  1:322149/1‑70 (MQ=255)

                                  tgtgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCggg                                                      <  1:1398041/55‑1 (MQ=255)

                                    tgCCGCTGTTGCGTATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTtgtg                                                 <  1:1139418/58‑1 (MQ=255)

                                    tgCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATg                                    >  1:2599270/1‑71 (MQ=255)

                                      ccGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTaa                                      >  1:3530304/1‑67 (MQ=255)

                                          tGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCt                               >  1:363305/1‑70 (MQ=255)

                                            ttGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATg                                          <  1:29745/57‑1 (MQ=255)

                                            ttGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTT‑CTCat                            <  1:2393005/70‑1 (MQ=255)

                                             tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCt                               >  1:2881427/1‑67 (MQ=255)

                                             tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCt                               >  1:1855616/1‑67 (MQ=255)

                                             tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCata                           >  1:2579112/1‑71 (MQ=255)

                                             tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCata                           >  1:2731512/1‑71 (MQ=255)

                                             tGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCata                           >  1:1013104/1‑71 (MQ=255)

                                               cGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                                             <  1:1213714/51‑1 (MQ=255)

                                                ggATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATaa                                             <  1:3132729/50‑1 (MQ=255)

                                                ggATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTaa                                      <  1:2820987/57‑1 (MQ=255)

                                                 gATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAg                       <  1:2906178/71‑1 (MQ=255)

                                                  atcatcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAg                       >  1:1012718/1‑70 (MQ=255)

                                                  atcatcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGt                      <  1:1984321/71‑1 (MQ=255)

                                                  atcatcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGt                      <  1:3667590/71‑1 (MQ=255)

                                                  agcatcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATc                         <  1:471410/66‑1 (MQ=255)

                                                  agcatcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAg                       >  1:3036614/3‑70 (MQ=255)

                                                  agcatcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGt                      <  1:2259287/69‑1 (MQ=255)

                                                   tcatcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTa                     >  1:1931336/1‑71 (MQ=255)

                                                    catcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTa                     >  1:2241728/1‑70 (MQ=255)

                                                     atcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGt                                   >  1:2378487/1‑55 (MQ=255)

                                                     atcTCCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGt                                   >  1:3055752/1‑55 (MQ=255)

                                                     atcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtc                   >  1:1636529/1‑71 (MQ=255)

                                                     atcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtc                   >  1:2552801/1‑71 (MQ=255)

                                                     atcTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtc                   >  1:3917528/1‑71 (MQ=255)

                                                      tctcCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGtt                                                    <  1:1749654/37‑1 (MQ=16)

                                                      tctcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtct                  >  1:2415416/1‑71 (MQ=255)

                                                      tctcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTAATATCAGTAtct                  >  1:3822883/1‑71 (MQ=255)

                                                      tctcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGT‑ATGTTGCTCATATCAGTAtct                  >  1:2076000/1‑70 (MQ=255)

                                                        tcCAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGAt                                           <  1:1714810/44‑1 (MQ=255)

                                                        tcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCa                >  1:756080/1‑71 (MQ=255)

                                                        tcCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCa                >  1:1889602/1‑71 (MQ=255)

                                                         ccAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCAt               >  1:740956/1‑71 (MQ=255)

                                                          cAGCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATCGTAATGTTGCTCATATCAGTAtct                  <  1:3723317/67‑1 (MQ=255)

                                                          cAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTAtctc                 <  1:2656478/68‑1 (MQ=255)

                                                            gCGTGTTGCGTGAGGTGCTACAGGCCGGGTTGTGATAGATGGTAATGTTGATCATATCAGTATCTAATTa             >  1:3424739/1‑70 (MQ=255)

                                                            gCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTAtctaa                >  1:1868750/1‑65 (MQ=255)

                                                            gCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTa             >  1:2326959/1‑70 (MQ=255)

                                                            gCGTGTTGCGTGAGGTGCCACAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTAc            <  1:2753213/71‑1 (MQ=255)

                                                            gCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCatat                          <  1:2495028/57‑1 (MQ=255)

                                                            gCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTa                     >  1:2126527/1‑62 (MQ=255)

                                                            gCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTa             >  1:1590420/1‑70 (MQ=255)

                                                             cGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACa           <  1:3436311/71‑1 (MQ=255)

                                                                   aCGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTg      >  1:3637173/1‑70 (MQ=255)

                                                                   aCGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTg      <  1:754711/70‑1 (MQ=255)

                                                                    cgcgACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTg      <  1:1221495/69‑1 (MQ=255)

                                                                    cgcgACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGa     >  1:168189/1‑70 (MQ=255)

                                                                     gcgACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAg                       >  1:275117/1‑51 (MQ=255)

                                                                      cgACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAag  <  1:1810013/71‑1 (MQ=255)

GCGGAGTTTCCAGATAATGGATAATAGTCGGTTCTGTGCCGCTGTTGCGGATCATCTCCAGCGTATTACGCGACGTGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAG  >  minE/2595181‑2595321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`