| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,724 | A→G | 9.0% | L383L (CTT→CTC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,724 | 0 | A | G | 9.0% | 257.9 / 12.8 | 93 | L383L (CTT→CTC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (33/51); new base G (3/5); total (36/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCT > minE/311658‑311794 | caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa < 1:2470315/71‑1 (MQ=255) agcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat < 1:2299073/69‑2 (MQ=12) agcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat > 1:2319320/3‑70 (MQ=12) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc < 1:2742190/71‑1 (MQ=255) gcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGatg > 1:3815727/2‑69 (MQ=12) gcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGatg > 1:576654/2‑69 (MQ=12) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:3497243/1‑71 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACgg > 1:3534739/1‑70 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2363271/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:1796073/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:3864917/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2124814/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:3006533/70‑1 (MQ=255) cccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCa < 1:690757/69‑1 (MQ=12) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:1565651/69‑1 (MQ=255) cGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCaaac < 1:3652167/71‑2 (MQ=16) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt > 1:1668109/1‑71 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt > 1:820374/1‑71 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt < 1:3732859/71‑1 (MQ=255) tGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc > 1:2077061/1‑71 (MQ=255) tGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc > 1:1306873/1‑71 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc > 1:3435692/1‑71 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:2907973/1‑58 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:762918/1‑60 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:3526958/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:2262432/71‑1 (MQ=255) aaGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat < 1:663174/56‑2 (MQ=9) gCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAg > 1:2540071/1‑70 (MQ=255) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:3730817/1‑55 (MQ=255) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:992243/1‑55 (MQ=255) aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa < 1:3568196/71‑1 (MQ=255) aCCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaa < 1:3435123/71‑1 (MQ=12) aTCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGa > 1:3509140/1‑64 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc < 1:865345/52‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc < 1:2946875/52‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATac < 1:1866087/70‑1 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc < 1:1104027/58‑1 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATacca < 1:742993/70‑1 (MQ=255) cGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:3615318/53‑1 (MQ=255) cGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:832086/53‑1 (MQ=255) cGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:2476207/53‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGGCGGCAAAGGTCCCGAGCAATACCACCg > 1:3583996/1‑71 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg < 1:2780201/71‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg < 1:3451732/71‑1 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:2634466/71‑1 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:1878588/71‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg > 1:1436073/1‑70 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:899232/70‑1 (MQ=255) cATTGTTTGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa < 1:716698/71‑1 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa > 1:41513/1‑71 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa > 1:313318/1‑71 (MQ=255) aTTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAc > 1:3030104/1‑71 (MQ=255) tGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGa > 1:774512/1‑53 (MQ=255) gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACgg > 1:537101/1‑70 (MQ=255) ttAGCGTGTTTATCGAGAAGCCAAAGGCGGCTAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGc < 1:1383837/70‑1 (MQ=255) ttAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc < 1:1533185/48‑1 (MQ=255) tAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa > 1:1543152/1‑54 (MQ=255) aGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATaccac > 1:1478909/1‑59 (MQ=255) cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATgg < 1:2288479/71‑1 (MQ=255) cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATgg < 1:1681432/71‑1 (MQ=255) cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATgg < 1:3819116/71‑1 (MQ=255) gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGt > 1:1594944/1‑71 (MQ=255) tgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:621452/35‑1 (MQ=255) tATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGa < 1:2700496/41‑1 (MQ=255) aTCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGaa > 1:300351/1‑70 (MQ=255) tCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAAt < 1:2453210/70‑1 (MQ=255) agaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCGATGGTCGGAATCAAc > 1:1445490/1‑71 (MQ=255) agaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAAc < 1:3123425/71‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCa < 1:573145/68‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCa < 1:811350/68‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg < 1:3945945/71‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg < 1:2862872/71‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg > 1:2081468/1‑71 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa > 1:1138492/1‑38 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGt > 1:464883/1‑71 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGt > 1:460268/1‑71 (MQ=255) gCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCgg < 1:3917453/60‑1 (MQ=255) gCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcg < 1:2149287/71‑1 (MQ=255) cAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATATCACCGGTACGGCAATGGTCGGAATCAACGTcgc < 1:1414779/70‑1 (MQ=255) cAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgc < 1:413555/70‑1 (MQ=255) aaaGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTc < 1:1522741/55‑1 (MQ=255) aaGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcgcg > 1:2744453/1‑71 (MQ=255) aaGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcgcg < 1:1799095/71‑1 (MQ=255) aaGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgc < 1:2247893/68‑1 (MQ=255) aaGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgc < 1:3907619/68‑1 (MQ=255) aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcgcg > 1:1885244/1‑70 (MQ=255) ggcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAAt > 1:2360096/1‑57 (MQ=255) ggcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGGCGGAATCAACGTCGCGCGGa < 1:2395896/71‑1 (MQ=255) ggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcg < 1:1670308/62‑1 (MQ=255) gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt < 1:869617/71‑1 (MQ=255) gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt < 1:2214056/71‑1 (MQ=255) gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt < 1:1888094/71‑1 (MQ=255) cAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcg > 1:1410075/1‑62 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGTCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:75692/71‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGaa < 1:1939158/50‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGaa < 1:2324492/50‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt > 1:173217/1‑71 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:1157785/71‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:96267/71‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt > 1:2433711/1‑71 (MQ=255) | CAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCT > minE/311658‑311794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |