| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,350 | C→G | 20.5% | V416V (GTG→GTC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,350 | 0 | C | G | 20.5% | 146.3 / 33.0 | 73 | V416V (GTG→GTC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (26/32); new base G (10/5); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
TAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCA > minE/2595283‑2595414 | tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTgaca < 1:820837/71‑1 (MQ=255) aTGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACaa < 1:3403041/70‑1 (MQ=255) tGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa > 1:3213169/1‑70 (MQ=255) tGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAAc < 1:3837190/71‑1 (MQ=255) gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:88415/67‑1 (MQ=255) gTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACaa > 1:1310177/1‑68 (MQ=255) tGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAg > 1:2276807/1‑70 (MQ=255) gCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGc < 1:3681216/70‑1 (MQ=255) cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca > 1:3836341/1‑70 (MQ=255) tCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGc < 1:3183512/68‑1 (MQ=255) tCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGcacc > 1:81486/1‑70 (MQ=255) cATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa > 1:559926/1‑63 (MQ=255) atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg > 1:429480/1‑71 (MQ=255) atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcag < 1:3586054/70‑1 (MQ=255) atatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACaaa < 1:3632121/62‑1 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca < 1:2755530/70‑1 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca < 1:383569/70‑1 (MQ=255) aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg > 1:10222/1‑71 (MQ=255) tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGt > 1:1847403/1‑70 (MQ=255) tctcTTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa < 1:2217803/70‑1 (MQ=255) cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa > 1:2259166/1‑70 (MQ=255) aatACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa < 1:3616152/69‑1 (MQ=255) aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac < 1:2522409/70‑2 (MQ=255) aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt < 1:3633394/67‑1 (MQ=255) aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt < 1:737032/67‑1 (MQ=255) ttACAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa < 1:3458706/71‑1 (MQ=255) ttACAAACTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa < 1:3817304/71‑1 (MQ=255) tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt > 1:2860903/1‑71 (MQ=255) cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc > 1:3770257/1‑71 (MQ=255) cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc < 1:2732030/71‑1 (MQ=255) cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc < 1:1120769/70‑1 (MQ=255) cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc < 1:2117169/70‑1 (MQ=255) aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc < 1:2263014/39‑1 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:3918337/1‑71 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3201099/71‑1 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:743724/71‑1 (MQ=255) gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg > 1:2285033/1‑68 (MQ=255) aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTAc < 1:483706/70‑1 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:3658085/71‑1 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg > 1:959590/1‑70 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3873682/65‑1 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:3161442/1‑70 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGa < 1:2954813/71‑1 (MQ=255) gagagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:2398976/1‑69 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:743734/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:1478869/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:3810850/71‑1 (MQ=255) aCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat < 1:274209/71‑1 (MQ=255) cGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat < 1:3560902/70‑1 (MQ=255) gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:1452255/1‑71 (MQ=255) gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:2573543/1‑71 (MQ=255) gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat < 1:43544/71‑1 (MQ=255) tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt < 1:2627965/55‑1 (MQ=255) tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATc > 1:3292052/1‑71 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:1454587/1‑71 (MQ=255) gcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:1856021/1‑69 (MQ=255) ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc < 1:1844456/48‑1 (MQ=255) cAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaag > 1:3491374/1‑69 (MQ=255) cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa > 1:1749108/1‑61 (MQ=255) cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGc > 1:206664/1‑71 (MQ=255) cgcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTa > 1:114326/2‑59 (MQ=255) aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt < 1:609362/71‑1 (MQ=255) gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt > 1:2844051/1‑67 (MQ=255) gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt > 1:3247024/1‑67 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:681841/1‑71 (MQ=255) cggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAtt > 1:2394515/3‑70 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa < 1:1171195/71‑1 (MQ=255) ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg > 1:1393225/2‑69 (MQ=255) agccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg > 1:1978763/1‑71 (MQ=255) gccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt > 1:163676/1‑71 (MQ=255) gccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGt < 1:2919751/71‑1 (MQ=255) ccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:3816696/66‑1 (MQ=255) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa < 1:3788270/48‑1 (MQ=255) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:1325139/1‑71 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAata > 1:3429149/1‑53 (MQ=255) cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:3780660/1‑52 (MQ=255) | TAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCA > minE/2595283‑2595414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |