Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,362 A→C 18.9% P412P (CCT→CCG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,3620AC18.9% 124.4 / 37.0 74P412P (CCT→CCGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (27/33);  new base C (12/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 7.25e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

ATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGT  >  minE/2595294‑2595431
                                                                    |                                                                     
atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg                                                                     >  1:429480/1‑71 (MQ=255)
atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcag                                                                      <  1:3586054/70‑1 (MQ=255)
   tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                                                                   <  1:2755530/70‑1 (MQ=255)
   tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                                                                   <  1:383569/70‑1 (MQ=255)
     aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                                                                >  1:10222/1‑71 (MQ=255)
       tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGt                                                               >  1:1847403/1‑70 (MQ=255)
         tctcTTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCa                                                             <  1:2217803/70‑1 (MQ=255)
            cATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                                          >  1:2259166/1‑70 (MQ=255)
             aatACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                                        <  1:3616152/69‑1 (MQ=255)
             aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                                         <  1:2522409/70‑2 (MQ=255)
             aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                            <  1:737032/67‑1 (MQ=255)
             aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                            <  1:3633394/67‑1 (MQ=255)
              ttACAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                                                       <  1:3458706/71‑1 (MQ=255)
              ttACAAACTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                                                       <  1:3817304/71‑1 (MQ=255)
               tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                                                      >  1:2860903/1‑71 (MQ=255)
                 cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                    <  1:2732030/71‑1 (MQ=255)
                 cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                    >  1:3770257/1‑71 (MQ=255)
                 cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                                     <  1:1120769/70‑1 (MQ=255)
                 cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                                     <  1:2117169/70‑1 (MQ=255)
                    aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                 >  1:3918337/1‑71 (MQ=255)
                    aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                 <  1:743724/71‑1 (MQ=255)
                    aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                 <  1:3201099/71‑1 (MQ=255)
                     gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg                                                 >  1:2285033/1‑68 (MQ=255)
                        aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTAc                                              <  1:483706/70‑1 (MQ=255)
                         aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                                            <  1:3658085/71‑1 (MQ=255)
                         aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg                                             >  1:959590/1‑70 (MQ=255)
                          agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                 <  1:3873682/65‑1 (MQ=255)
                          agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                                            >  1:3161442/1‑70 (MQ=255)
                          agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGa                                           <  1:2954813/71‑1 (MQ=255)
                           gagagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                                            >  1:2398976/1‑69 (MQ=255)
                             gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                        <  1:743734/71‑1 (MQ=255)
                              agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                       <  1:1478869/71‑1 (MQ=255)
                              agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                       <  1:3810850/71‑1 (MQ=255)
                                aCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat                                     <  1:274209/71‑1 (MQ=255)
                                 cGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat                                     <  1:3560902/70‑1 (MQ=255)
                                  gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat                                   <  1:43544/71‑1 (MQ=255)
                                  gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat                                   >  1:2573543/1‑71 (MQ=255)
                                  gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat                                   >  1:1452255/1‑71 (MQ=255)
                                   tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt                                                  <  1:2627965/55‑1 (MQ=255)
                                   tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATc                                  >  1:3292052/1‑71 (MQ=255)
                                     gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                >  1:1454587/1‑71 (MQ=255)
                                       gcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                >  1:1856021/1‑69 (MQ=255)
                                        ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                    <  1:1844456/48‑1 (MQ=255)
                                         cAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaag                            >  1:3491374/1‑69 (MQ=255)
                                         cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa                                      >  1:1749108/1‑61 (MQ=255)
                                         cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGc                            >  1:206664/1‑71 (MQ=255)
                                          cgcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTa                                       >  1:114326/2‑59 (MQ=255)
                                          aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt                           <  1:609362/71‑1 (MQ=255)
                                           gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt                           >  1:3247024/1‑67 (MQ=255)
                                           gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt                           >  1:2844051/1‑67 (MQ=255)
                                            cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                         >  1:681841/1‑71 (MQ=255)
                                              cggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAtt                       >  1:2394515/3‑70 (MQ=255)
                                              cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa                       <  1:1171195/71‑1 (MQ=255)
                                               ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg                      >  1:1393225/2‑69 (MQ=255)
                                               agccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg                      >  1:1978763/1‑71 (MQ=255)
                                                gccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                     >  1:163676/1‑71 (MQ=255)
                                                gccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGt                     <  1:2919751/71‑1 (MQ=255)
                                                 ccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                         <  1:3816696/66‑1 (MQ=255)
                                                  cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                          <  1:3788270/48‑1 (MQ=255)
                                                  cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                   >  1:1325139/1‑71 (MQ=255)
                                                   agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAata                                    >  1:3429149/1‑53 (MQ=255)
                                                     cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat                                   >  1:3780660/1‑52 (MQ=255)
                                                         acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt            >  1:1836209/1‑71 (MQ=255)
                                                         acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                          >  1:774322/1‑41 (MQ=255)
                                                         acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                          >  1:2052139/1‑41 (MQ=255)
                                                         acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                >  1:2133865/1‑51 (MQ=255)
                                                         acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                 <  1:636647/50‑1 (MQ=255)
                                                         acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                 <  1:2022787/50‑1 (MQ=255)
                                                         acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa                       <  1:1452662/60‑1 (MQ=255)
                                                         acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                 >  1:468464/1‑66 (MQ=255)
                                                          caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc           >  1:110598/1‑71 (MQ=255)
                                                            aaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg         >  1:470834/1‑71 (MQ=255)
                                                            aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc           <  1:3412815/69‑1 (MQ=255)
                                                            aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc           <  1:2816182/69‑1 (MQ=255)
                                                               aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg       >  1:3946538/1‑68 (MQ=255)
                                                               aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg       >  1:244248/1‑68 (MQ=255)
                                                               aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                        <  1:2123610/37‑1 (MQ=255)
                                                               aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCg       >  1:900169/1‑70 (MQ=255)
                                                                  acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg   <  1:551815/71‑1 (MQ=255)
                                                                  acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg   <  1:1270859/71‑1 (MQ=255)
                                                                   caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg   >  1:2450722/1‑70 (MQ=255)
                                                                   caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg   >  1:1122091/1‑70 (MQ=255)
                                                                   caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt  <  1:1360995/71‑1 (MQ=255)
                                                                   caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt  <  1:982072/71‑1 (MQ=255)
                                                                    |                                                                     
ATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGT  >  minE/2595294‑2595431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: