Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,376 T→C 19.3% I408V (ATC→GTC)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,3760TC19.3% 185.7 / 37.4 88I408V (ATC→GTC) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (35/36);  new base C (14/3);  total (49/39)
Fisher's exact test for biased strand distribution p-value = 1.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAG  >  minE/2595307‑2595445
                                                                     |                                                                     
aatACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                                                      <  1:3616152/69‑1 (MQ=255)
 ttACAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                                                                     <  1:3458706/71‑1 (MQ=255)
 ttACAAACTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                                                                     <  1:3817304/71‑1 (MQ=255)
  tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                                                                    >  1:2860903/1‑71 (MQ=255)
    cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                                  >  1:3770257/1‑71 (MQ=255)
    cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                                  <  1:2732030/71‑1 (MQ=255)
    cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                                                   <  1:1120769/70‑1 (MQ=255)
    cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                                                   <  1:2117169/70‑1 (MQ=255)
       aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                               >  1:3918337/1‑71 (MQ=255)
       aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                               <  1:3201099/71‑1 (MQ=255)
       aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                               <  1:743724/71‑1 (MQ=255)
        gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg                                                               >  1:2285033/1‑68 (MQ=255)
           aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTAc                                                            <  1:483706/70‑1 (MQ=255)
            aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                                                          <  1:3658085/71‑1 (MQ=255)
            aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg                                                           >  1:959590/1‑70 (MQ=255)
             agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                               <  1:3873682/65‑1 (MQ=255)
             agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                                                          >  1:3161442/1‑70 (MQ=255)
             agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGa                                                         <  1:2954813/71‑1 (MQ=255)
              gagagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg                                                          >  1:2398976/1‑69 (MQ=255)
                gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                                      <  1:743734/71‑1 (MQ=255)
                 agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                                     <  1:1478869/71‑1 (MQ=255)
                 agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                                     <  1:3810850/71‑1 (MQ=255)
                   aCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat                                                   <  1:274209/71‑1 (MQ=255)
                    cGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat                                                   <  1:3560902/70‑1 (MQ=255)
                     gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat                                                 >  1:1452255/1‑71 (MQ=255)
                     gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat                                                 <  1:43544/71‑1 (MQ=255)
                     gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat                                                 >  1:2573543/1‑71 (MQ=255)
                      tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt                                                                <  1:2627965/55‑1 (MQ=255)
                      tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATc                                                >  1:3292052/1‑71 (MQ=255)
                        gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                              >  1:1454587/1‑71 (MQ=255)
                          gcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                              >  1:1856021/1‑69 (MQ=255)
                           ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                                  <  1:1844456/48‑1 (MQ=255)
                            cAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaag                                          >  1:3491374/1‑69 (MQ=255)
                            cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa                                                    >  1:1749108/1‑61 (MQ=255)
                            cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGc                                          >  1:206664/1‑71 (MQ=255)
                             cgcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTa                                                     >  1:114326/2‑59 (MQ=255)
                             aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt                                         <  1:609362/71‑1 (MQ=255)
                              gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt                                         >  1:3247024/1‑67 (MQ=255)
                              gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt                                         >  1:2844051/1‑67 (MQ=255)
                               cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                       >  1:681841/1‑71 (MQ=255)
                                 cggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAtt                                     >  1:2394515/3‑70 (MQ=255)
                                 cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa                                     <  1:1171195/71‑1 (MQ=255)
                                  ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg                                    >  1:1393225/2‑69 (MQ=255)
                                  agccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg                                    >  1:1978763/1‑71 (MQ=255)
                                   gccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                                   >  1:163676/1‑71 (MQ=255)
                                   gccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGt                                   <  1:2919751/71‑1 (MQ=255)
                                    ccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                       <  1:3816696/66‑1 (MQ=255)
                                     cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                        <  1:3788270/48‑1 (MQ=255)
                                     cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                                 >  1:1325139/1‑71 (MQ=255)
                                      agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAata                                                  >  1:3429149/1‑53 (MQ=255)
                                        cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat                                                 >  1:3780660/1‑52 (MQ=255)
                                            acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt                          >  1:1836209/1‑71 (MQ=255)
                                            acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                        >  1:774322/1‑41 (MQ=255)
                                            acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                        >  1:2052139/1‑41 (MQ=255)
                                            acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                              >  1:2133865/1‑51 (MQ=255)
                                            acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                               <  1:636647/50‑1 (MQ=255)
                                            acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                               <  1:2022787/50‑1 (MQ=255)
                                            acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa                                     <  1:1452662/60‑1 (MQ=255)
                                            acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                               >  1:468464/1‑66 (MQ=255)
                                             caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc                         >  1:110598/1‑71 (MQ=255)
                                               aaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg                       >  1:470834/1‑71 (MQ=255)
                                               aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc                         <  1:2816182/69‑1 (MQ=255)
                                               aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc                         <  1:3412815/69‑1 (MQ=255)
                                                  aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg                     >  1:3946538/1‑68 (MQ=255)
                                                  aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg                     >  1:244248/1‑68 (MQ=255)
                                                  aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                                      <  1:2123610/37‑1 (MQ=255)
                                                  aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCg                     >  1:900169/1‑70 (MQ=255)
                                                     acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                 <  1:1270859/71‑1 (MQ=255)
                                                     acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                 <  1:551815/71‑1 (MQ=255)
                                                      caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                 >  1:2450722/1‑70 (MQ=255)
                                                      caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                 >  1:1122091/1‑70 (MQ=255)
                                                      caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt                <  1:982072/71‑1 (MQ=255)
                                                      caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt                <  1:1360995/71‑1 (MQ=255)
                                                        ggcaggGTCATGATAATCCCTGTACGGAAGTAATATc                                                >  1:3410296/1‑37 (MQ=255)
                                                        ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc              >  1:505196/1‑71 (MQ=255)
                                                        ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc              >  1:3790670/1‑71 (MQ=255)
                                                          caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt            >  1:2703927/1‑71 (MQ=255)
                                                           aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg                                    <  1:3383163/46‑1 (MQ=255)
                                                           aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTg           >  1:1778612/1‑71 (MQ=255)
                                                            cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc          <  1:2889520/69‑1 (MQ=255)
                                                            cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc          <  1:1469196/69‑1 (MQ=255)
                                                             agTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg           >  1:263311/2‑69 (MQ=255)
                                                               tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGAt                                      <  1:2653800/40‑1 (MQ=255)
                                                                cATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                            >  1:434815/1‑49 (MQ=255)
                                                                 aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc       >  1:3671796/1‑69 (MQ=255)
                                                                 aTGACAATGCCGGTACGGAAGTAATATCCACAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa     >  1:2030701/1‑71 (MQ=255)
                                                                   gATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                                 >  1:3665270/1‑41 (MQ=255)
                                                                    aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt            >  1:3574714/1‑61 (MQ=255)
                                                                     tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag  >  1:2790014/1‑70 (MQ=255)
                                                                     |                                                                     
ATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAG  >  minE/2595307‑2595445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: