| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,383 | T→G | 19.3% | T405T (ACA→ACC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,383 | 0 | T | G | 19.3% | 144.9 / 47.1 | 88 | T405T (ACA→ACC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (35/36); new base G (14/3); total (49/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
AGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTA > minE/2595314‑2595452 | aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:3918337/1‑71 (MQ=255)aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3201099/71‑1 (MQ=255)aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:743724/71‑1 (MQ=255) aaaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTAc < 1:483706/70‑1 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg < 1:3658085/71‑1 (MQ=255) aaGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACg > 1:959590/1‑70 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3873682/65‑1 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:3161442/1‑70 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGa < 1:2954813/71‑1 (MQ=255) gagagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACgg > 1:2398976/1‑69 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:743734/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:1478869/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:3810850/71‑1 (MQ=255) aCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat < 1:274209/71‑1 (MQ=255) cGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAat < 1:3560902/70‑1 (MQ=255) gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:2573543/1‑71 (MQ=255) gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat < 1:43544/71‑1 (MQ=255) gTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:1452255/1‑71 (MQ=255) tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt < 1:2627965/55‑1 (MQ=255) tAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATc > 1:3292052/1‑71 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:1454587/1‑71 (MQ=255) gcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:1856021/1‑69 (MQ=255) cAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaag > 1:3491374/1‑69 (MQ=255) cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTaa > 1:1749108/1‑61 (MQ=255) cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGc > 1:206664/1‑71 (MQ=255) cgcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTa > 1:114326/2‑59 (MQ=255) aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt < 1:609362/71‑1 (MQ=255) gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt > 1:2844051/1‑67 (MQ=255) gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCaagt > 1:3247024/1‑67 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:681841/1‑71 (MQ=255) cggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAtt > 1:2394515/3‑70 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa < 1:1171195/71‑1 (MQ=255) ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg > 1:1393225/2‑69 (MQ=255) agccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg > 1:1978763/1‑71 (MQ=255) gccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt > 1:163676/1‑71 (MQ=255) gccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGt < 1:2919751/71‑1 (MQ=255) ccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:3816696/66‑1 (MQ=255) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa < 1:3788270/48‑1 (MQ=255) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:1325139/1‑71 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAata > 1:3429149/1‑53 (MQ=255) cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAatat > 1:3780660/1‑52 (MQ=255) acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt > 1:1836209/1‑71 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa > 1:2052139/1‑41 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa > 1:774322/1‑41 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:2133865/1‑51 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc < 1:636647/50‑1 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc < 1:2022787/50‑1 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa < 1:1452662/60‑1 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata > 1:468464/1‑66 (MQ=255) caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc > 1:110598/1‑71 (MQ=255) aaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg > 1:470834/1‑71 (MQ=255) aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:2816182/69‑1 (MQ=255) aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:3412815/69‑1 (MQ=255) aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:3946538/1‑68 (MQ=255) aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:244248/1‑68 (MQ=255) aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:2123610/37‑1 (MQ=255) aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCg > 1:900169/1‑70 (MQ=255) acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:551815/71‑1 (MQ=255) acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:1270859/71‑1 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:1122091/1‑70 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:2450722/1‑70 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:982072/71‑1 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:1360995/71‑1 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATc > 1:3410296/1‑37 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc > 1:505196/1‑71 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc > 1:3790670/1‑71 (MQ=255) caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt > 1:2703927/1‑71 (MQ=255) aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg < 1:3383163/46‑1 (MQ=255) aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTg > 1:1778612/1‑71 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc < 1:1469196/69‑1 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc < 1:2889520/69‑1 (MQ=255) agTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg > 1:263311/2‑69 (MQ=255) tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGAt < 1:2653800/40‑1 (MQ=255) cATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc > 1:434815/1‑49 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc > 1:3671796/1‑69 (MQ=255) aTGACAATGCCGGTACGGAAGTAATATCCACAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa > 1:2030701/1‑71 (MQ=255) gATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:3665270/1‑41 (MQ=255) aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt > 1:3574714/1‑61 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag > 1:2790014/1‑70 (MQ=255) caaTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt > 1:1889788/2‑43 (MQ=16) aTCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta < 1:3827742/70‑1 (MQ=255) tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:1824913/1‑71 (MQ=255) tCCCTCTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa < 1:3702156/50‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:296698/51‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:858180/51‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:2868312/70‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:2598718/70‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:3036123/70‑1 (MQ=255) cTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta < 1:2629019/71‑1 (MQ=255) tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg < 1:3016913/52‑1 (MQ=255) tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:3679146/60‑1 (MQ=255) | AGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTA > minE/2595314‑2595452 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |