| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,405 | C→G | 21.8% | S398T (AGC→ACC) ‡ | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,405 | 0 | C | G | 21.8% | 154.5 / 37.8 | 78 | S398T (AGC→ACC) ‡ | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (33/28); new base G (12/5); total (45/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
CAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGT > minE/2595335‑2595472 | cAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGc > 1:206664/1‑71 (MQ=255) aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt < 1:609362/71‑1 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:681841/1‑71 (MQ=255) cggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAtt > 1:2394515/3‑70 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa < 1:1171195/71‑1 (MQ=255) ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg > 1:1393225/2‑69 (MQ=255) agccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg > 1:1978763/1‑71 (MQ=255) gccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt > 1:163676/1‑71 (MQ=255) gccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGt < 1:2919751/71‑1 (MQ=255) ccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:3816696/66‑1 (MQ=255) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:1325139/1‑71 (MQ=255) acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt > 1:1836209/1‑71 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATa < 1:1452662/60‑1 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata > 1:468464/1‑66 (MQ=255) caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc > 1:110598/1‑71 (MQ=255) aaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg > 1:470834/1‑71 (MQ=255) aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:3412815/69‑1 (MQ=255) aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc < 1:2816182/69‑1 (MQ=255) aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:3946538/1‑68 (MQ=255) aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:244248/1‑68 (MQ=255) aGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCg > 1:900169/1‑70 (MQ=255) acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:551815/71‑1 (MQ=255) acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg < 1:1270859/71‑1 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:2450722/1‑70 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:1122091/1‑70 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:982072/71‑1 (MQ=255) caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:1360995/71‑1 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc > 1:3790670/1‑71 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc > 1:505196/1‑71 (MQ=255) caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt > 1:2703927/1‑71 (MQ=255) aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAg < 1:3383163/46‑1 (MQ=255) aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTg > 1:1778612/1‑71 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc < 1:1469196/69‑1 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc < 1:2889520/69‑1 (MQ=255) agTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg > 1:263311/2‑69 (MQ=255) tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGAt < 1:2653800/40‑1 (MQ=255) cATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc > 1:434815/1‑49 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc > 1:3671796/1‑69 (MQ=255) aTGACAATGCCGGTACGGAAGTAATATCCACAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa > 1:2030701/1‑71 (MQ=255) gATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:3665270/1‑41 (MQ=255) aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt > 1:3574714/1‑61 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag > 1:2790014/1‑70 (MQ=255) caaTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt > 1:1889788/2‑43 (MQ=16) aTCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta < 1:3827742/70‑1 (MQ=255) tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:1824913/1‑71 (MQ=255) tCCCTCTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa < 1:3702156/50‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:858180/51‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt < 1:296698/51‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:2598718/70‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:2868312/70‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:3036123/70‑1 (MQ=255) cTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta < 1:2629019/71‑1 (MQ=255) tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg < 1:3016913/52‑1 (MQ=255) tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:3679146/60‑1 (MQ=255) tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTg > 1:1030906/1‑52 (MQ=255) tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc < 1:3657341/70‑1 (MQ=255) aCGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:2102462/70‑1 (MQ=255) aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc > 1:490719/1‑69 (MQ=255) cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:1364978/1‑46 (MQ=255) cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:3348175/47‑1 (MQ=255) cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg > 1:1862784/1‑71 (MQ=255) cGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCa < 1:2154974/70‑1 (MQ=255) ggAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAga < 1:2259493/71‑2 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg > 1:582783/1‑46 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:673127/67‑1 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:1259938/67‑1 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg > 1:758049/1‑69 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:213929/1‑71 (MQ=255) gAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAga > 1:111361/1‑69 (MQ=255) aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:1297605/1‑70 (MQ=255) aGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAg > 1:1698358/1‑49 (MQ=255) gTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc > 1:1992439/1‑71 (MQ=255) atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc > 1:1670138/1‑68 (MQ=255) atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa > 1:3642314/1‑71 (MQ=255) tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt < 1:3667264/70‑1 (MQ=255) tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg > 1:3914733/1‑71 (MQ=255) ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga > 1:793943/1‑46 (MQ=255) aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt > 1:3123238/1‑70 (MQ=255) aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt > 1:2460920/1‑70 (MQ=255) | CAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGT > minE/2595335‑2595472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |