Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,427 A→C 9.7% S391A (TCG→GCG) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,4270AC 9.7% 192.2 / 15.2 82S391A (TCG→GCG) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (39/35);  new base C (4/4);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGC  >  minE/2595360‑2595495
                                                                   |                                                                    
acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                   <  1:551815/71‑1 (MQ=255)
acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                   <  1:1270859/71‑1 (MQ=255)
 caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                   >  1:1122091/1‑70 (MQ=255)
 caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                   >  1:2450722/1‑70 (MQ=255)
 caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt                                                                  <  1:982072/71‑1 (MQ=255)
 caggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt                                                                  <  1:1360995/71‑1 (MQ=255)
   ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                                >  1:505196/1‑71 (MQ=255)
   ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                                >  1:3790670/1‑71 (MQ=255)
     caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                                                              >  1:2703927/1‑71 (MQ=255)
      aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTg                                                             >  1:1778612/1‑71 (MQ=255)
       cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                                                            <  1:1469196/69‑1 (MQ=255)
       cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc                                                            <  1:2889520/69‑1 (MQ=255)
        agTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                             >  1:263311/2‑69 (MQ=255)
            aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc                                                         >  1:3671796/1‑69 (MQ=255)
            aTGACAATGCCGGTACGGAAGTAATATCCACAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa                                                       >  1:2030701/1‑71 (MQ=255)
               aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                                                              >  1:3574714/1‑61 (MQ=255)
                tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                    >  1:2790014/1‑70 (MQ=255)
                  aTCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                  <  1:3827742/70‑1 (MQ=255)
                   tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                >  1:1824913/1‑71 (MQ=255)
                   tCCCTCTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa                                                                     <  1:3702156/50‑1 (MQ=255)
                     ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt                                                                  <  1:296698/51‑1 (MQ=255)
                     ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGt                                                                  <  1:858180/51‑1 (MQ=255)
                     ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                               <  1:3036123/70‑1 (MQ=255)
                     ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                               <  1:2868312/70‑1 (MQ=255)
                     ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                               <  1:2598718/70‑1 (MQ=255)
                      cTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                             <  1:2629019/71‑1 (MQ=255)
                       tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg                                                               <  1:3016913/52‑1 (MQ=255)
                       tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                                       <  1:3679146/60‑1 (MQ=255)
                         tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTg                                                             >  1:1030906/1‑52 (MQ=255)
                         tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                           <  1:3657341/70‑1 (MQ=255)
                          aCGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                          <  1:2102462/70‑1 (MQ=255)
                          aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                                           >  1:490719/1‑69 (MQ=255)
                           cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                                 >  1:1364978/1‑46 (MQ=255)
                           cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                                <  1:3348175/47‑1 (MQ=255)
                           cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                        >  1:1862784/1‑71 (MQ=255)
                           cGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCa                                         <  1:2154974/70‑1 (MQ=255)
                            ggAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAga                                       <  1:2259493/71‑2 (MQ=255)
                             gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg                                                               >  1:582783/1‑46 (MQ=255)
                             gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                          <  1:1259938/67‑1 (MQ=255)
                             gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                          <  1:673127/67‑1 (MQ=255)
                             gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                        >  1:758049/1‑69 (MQ=255)
                             gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                      >  1:213929/1‑71 (MQ=255)
                             gAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAga                                       >  1:111361/1‑69 (MQ=255)
                              aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                      >  1:1297605/1‑70 (MQ=255)
                               aGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAg                                                          >  1:1698358/1‑49 (MQ=255)
                                gTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc                                   >  1:1992439/1‑71 (MQ=255)
                                   atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc                                   >  1:1670138/1‑68 (MQ=255)
                                   atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                >  1:3642314/1‑71 (MQ=255)
                                      tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt                              <  1:3667264/70‑1 (MQ=255)
                                      tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                             >  1:3914733/1‑71 (MQ=255)
                                       ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                     >  1:793943/1‑46 (MQ=255)
                                           aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                         >  1:3123238/1‑70 (MQ=255)
                                           aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                         >  1:2460920/1‑70 (MQ=255)
                                               gATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                  >  1:1474448/1‑41 (MQ=255)
                                                aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                    <  1:1179279/70‑1 (MQ=255)
                                                aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                    <  1:1167928/70‑1 (MQ=255)
                                                 tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                             <  1:3552625/44‑1 (MQ=255)
                                                 tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                  >  1:978495/1‑71 (MQ=255)
                                                 tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                  <  1:2374378/71‑1 (MQ=255)
                                                  aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgt                                              >  1:1510688/1‑42 (MQ=255)
                                                   gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                           >  1:3358788/1‑44 (MQ=255)
                                                   gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg                <  1:3285187/71‑1 (MQ=255)
                                                    tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc                                   <  1:3633233/51‑1 (MQ=255)
                                                    tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc                                   <  1:365947/51‑1 (MQ=255)
                                                      atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTc              <  1:695482/70‑1 (MQ=255)
                                                       tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                             >  1:482176/1‑54 (MQ=255)
                                                       tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc             >  1:3678810/1‑70 (MQ=255)
                                                        atTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc            >  1:769487/1‑70 (MQ=255)
                                                         ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                          <  1:3822538/39‑1 (MQ=255)
                                                         ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa          <  1:2313497/71‑1 (MQ=255)
                                                           cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa         <  1:3880676/70‑1 (MQ=255)
                                                            ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                 <  1:814116/61‑1 (MQ=255)
                                                            ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc       >  1:3061286/1‑71 (MQ=255)
                                                             tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                  >  1:2638405/1‑59 (MQ=255)
                                                              ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg      <  1:1535688/70‑1 (MQ=255)
                                                              ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg      <  1:2438638/70‑1 (MQ=255)
                                                              ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg      >  1:2872330/1‑70 (MQ=255)
                                                              ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg      <  1:1361854/70‑1 (MQ=255)
                                                               tGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg      >  1:1985086/1‑69 (MQ=255)
                                                                gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGACTACCAATTGGGGTAATTTTCGGTCCCAAATc       <  1:3885812/67‑1 (MQ=255)
                                                                  gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                     >  1:3457010/1‑51 (MQ=255)
                                                                  gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc  >  1:469164/1‑70 (MQ=255)
                                                                  gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc  >  1:3683788/1‑70 (MQ=255)
                                                                   aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc  >  1:744154/1‑69 (MQ=255)
                                                                   aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc  >  1:1781988/1‑69 (MQ=255)
                                                                   |                                                                    
ACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGC  >  minE/2595360‑2595495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: