| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,443 | G→C | 8.3% | L385L (CTC→CTG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,443 | 0 | G | C | 8.3% | 242.5 / 10.1 | 84 | L385L (CTC→CTG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (39/38); new base C (5/2); total (44/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
TAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATA > minE/2595376‑2595511 | tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag > 1:2790014/1‑70 (MQ=255) aTCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta < 1:3827742/70‑1 (MQ=255) tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:1824913/1‑71 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:2598718/70‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:3036123/70‑1 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg < 1:2868312/70‑1 (MQ=255) cTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta < 1:2629019/71‑1 (MQ=255) tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc < 1:3657341/70‑1 (MQ=255) aCGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:2102462/70‑1 (MQ=255) aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc > 1:490719/1‑69 (MQ=255) cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg > 1:1862784/1‑71 (MQ=255) cGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCa < 1:2154974/70‑1 (MQ=255) ggAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAga < 1:2259493/71‑2 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:1259938/67‑1 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:673127/67‑1 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg > 1:758049/1‑69 (MQ=255) gAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:213929/1‑71 (MQ=255) gAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAga > 1:111361/1‑69 (MQ=255) aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:1297605/1‑70 (MQ=255) gTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc > 1:1992439/1‑71 (MQ=255) atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc > 1:1670138/1‑68 (MQ=255) atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa > 1:3642314/1‑71 (MQ=255) tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt < 1:3667264/70‑1 (MQ=255) tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg > 1:3914733/1‑71 (MQ=255) ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga > 1:793943/1‑46 (MQ=255) aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt > 1:3123238/1‑70 (MQ=255) aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt > 1:2460920/1‑70 (MQ=255) gATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:1474448/1‑41 (MQ=255) aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt < 1:1179279/70‑1 (MQ=255) aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt < 1:1167928/70‑1 (MQ=255) tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta < 1:3552625/44‑1 (MQ=255) tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:978495/1‑71 (MQ=255) tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc < 1:2374378/71‑1 (MQ=255) aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgt > 1:1510688/1‑42 (MQ=255) gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc > 1:3358788/1‑44 (MQ=255) gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg < 1:3285187/71‑1 (MQ=255) tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc < 1:365947/51‑1 (MQ=255) tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc < 1:3633233/51‑1 (MQ=255) atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTc < 1:695482/70‑1 (MQ=255) tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg > 1:482176/1‑54 (MQ=255) tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc > 1:3678810/1‑70 (MQ=255) atTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc > 1:769487/1‑70 (MQ=255) ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc < 1:3822538/39‑1 (MQ=255) ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa < 1:2313497/71‑1 (MQ=255) cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa < 1:3880676/70‑1 (MQ=255) ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg < 1:814116/61‑1 (MQ=255) ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc > 1:3061286/1‑71 (MQ=255) tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:2638405/1‑59 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg > 1:2872330/1‑70 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:1361854/70‑1 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:1535688/70‑1 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:2438638/70‑1 (MQ=255) tGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg > 1:1985086/1‑69 (MQ=255) gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGACTACCAATTGGGGTAATTTTCGGTCCCAAATc < 1:3885812/67‑1 (MQ=255) gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt > 1:3457010/1‑51 (MQ=255) gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:3683788/1‑70 (MQ=255) gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:469164/1‑70 (MQ=255) aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:744154/1‑69 (MQ=255) aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:1781988/1‑69 (MQ=255) aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa > 1:1406051/1‑71 (MQ=255) aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg > 1:1296072/1‑40 (MQ=255) aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg > 1:3604441/1‑40 (MQ=255) aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt > 1:1530414/1‑49 (MQ=255) gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc < 1:1687020/71‑1 (MQ=255) aCGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca > 1:1281937/1‑70 (MQ=255) catGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcaca > 1:751980/3‑71 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg > 1:2788713/1‑48 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:3130744/71‑1 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca > 1:1926630/1‑71 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:272622/71‑1 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:1997930/1‑46 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCa < 1:114021/53‑1 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt < 1:3619878/71‑1 (MQ=255) tGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt < 1:2537102/71‑1 (MQ=255) gaaGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:3682967/3‑71 (MQ=255) gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:1279832/1‑71 (MQ=255) gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:328935/1‑71 (MQ=255) gCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg > 1:3239388/1‑71 (MQ=255) gCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg > 1:3045573/1‑71 (MQ=255) cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGTGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:1087227/70‑1 (MQ=255) cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:831074/70‑1 (MQ=255) cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:1043247/70‑1 (MQ=255) aGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:2735754/69‑1 (MQ=255) gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc < 1:3476446/52‑1 (MQ=255) ccAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCa > 1:2097381/1‑70 (MQ=255) cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa < 1:3680750/71‑1 (MQ=255) agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa > 1:927278/1‑70 (MQ=255) gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:2372881/61‑1 (MQ=255) gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt > 1:533963/1‑63 (MQ=255) gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt > 1:875123/1‑63 (MQ=255) | TAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATA > minE/2595376‑2595511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |