Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,461 A→G 6.2% G379G (GGT→GGC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,4610AG 6.2% 231.0 / 4.9 81G379G (GGT→GGCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (41/35);  new base G (3/2);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

GTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAT  >  minE/2595392‑2595529
                                                                     |                                                                    
gTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc                                                                     >  1:1992439/1‑71 (MQ=255)
   atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc                                                                     >  1:1670138/1‑68 (MQ=255)
   atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                                                  >  1:3642314/1‑71 (MQ=255)
      tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt                                                                <  1:3667264/70‑1 (MQ=255)
      tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                                               >  1:3914733/1‑71 (MQ=255)
           aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                                                           >  1:3123238/1‑70 (MQ=255)
           aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                                                           >  1:2460920/1‑70 (MQ=255)
                aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                                                      <  1:1167928/70‑1 (MQ=255)
                aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                                                      <  1:1179279/70‑1 (MQ=255)
                 tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                    >  1:978495/1‑71 (MQ=255)
                 tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                    <  1:2374378/71‑1 (MQ=255)
                   gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg                                                  <  1:3285187/71‑1 (MQ=255)
                    tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc                                                                     <  1:3633233/51‑1 (MQ=255)
                    tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTAc                                                                     <  1:365947/51‑1 (MQ=255)
                      atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTc                                                <  1:695482/70‑1 (MQ=255)
                       tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                                               >  1:482176/1‑54 (MQ=255)
                       tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                               >  1:3678810/1‑70 (MQ=255)
                        atTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                                              >  1:769487/1‑70 (MQ=255)
                         ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                                            <  1:2313497/71‑1 (MQ=255)
                           cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa                                           <  1:3880676/70‑1 (MQ=255)
                            ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                   <  1:814116/61‑1 (MQ=255)
                            ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                         >  1:3061286/1‑71 (MQ=255)
                             tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                    >  1:2638405/1‑59 (MQ=255)
                              ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                        >  1:2872330/1‑70 (MQ=255)
                              ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                        <  1:2438638/70‑1 (MQ=255)
                              ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                        <  1:1361854/70‑1 (MQ=255)
                              ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                        <  1:1535688/70‑1 (MQ=255)
                               tGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                        >  1:1985086/1‑69 (MQ=255)
                                gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGACTACCAATTGGGGTAATTTTCGGTCCCAAATc                                         <  1:3885812/67‑1 (MQ=255)
                                  gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                                                       >  1:3457010/1‑51 (MQ=255)
                                  gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                                    >  1:469164/1‑70 (MQ=255)
                                  gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                                    >  1:3683788/1‑70 (MQ=255)
                                   aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                                    >  1:744154/1‑69 (MQ=255)
                                   aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                                    >  1:1781988/1‑69 (MQ=255)
                                    aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                 >  1:1406051/1‑71 (MQ=255)
                                     aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                                               >  1:1296072/1‑40 (MQ=255)
                                     aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                                                               >  1:3604441/1‑40 (MQ=255)
                                     aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                                                      >  1:1530414/1‑49 (MQ=255)
                                      gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc                               <  1:1687020/71‑1 (MQ=255)
                                        aCGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                              >  1:1281937/1‑70 (MQ=255)
                                         catGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcaca                            >  1:751980/3‑71 (MQ=255)
                                         cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                   >  1:2788713/1‑48 (MQ=255)
                                         cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca                            <  1:3130744/71‑1 (MQ=255)
                                         cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca                            >  1:1926630/1‑71 (MQ=255)
                                         cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca                            <  1:272622/71‑1 (MQ=255)
                                          gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                    >  1:1997930/1‑46 (MQ=255)
                                          gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                             <  1:114021/53‑1 (MQ=255)
                                          gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt                           <  1:3619878/71‑1 (MQ=255)
                                           tGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt                          <  1:2537102/71‑1 (MQ=255)
                                            gaaGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                         >  1:3682967/3‑71 (MQ=255)
                                            gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                         >  1:1279832/1‑71 (MQ=255)
                                            gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                         >  1:328935/1‑71 (MQ=255)
                                            gCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg                         >  1:3239388/1‑71 (MQ=255)
                                            gCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg                         >  1:3045573/1‑71 (MQ=255)
                                             cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGTGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                         <  1:1087227/70‑1 (MQ=255)
                                             cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                         <  1:1043247/70‑1 (MQ=255)
                                             cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                         <  1:831074/70‑1 (MQ=255)
                                              aGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg                         <  1:2735754/69‑1 (MQ=255)
                                               gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                         <  1:3476446/52‑1 (MQ=255)
                                                ccAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCa                      >  1:2097381/1‑70 (MQ=255)
                                                 cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                    <  1:3680750/71‑1 (MQ=255)
                                                  agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                    >  1:927278/1‑70 (MQ=255)
                                                   gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca                            <  1:2372881/61‑1 (MQ=255)
                                                   gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt                          >  1:875123/1‑63 (MQ=255)
                                                   gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt                          >  1:533963/1‑63 (MQ=255)
                                                    agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaaa                  >  1:2420487/1‑70 (MQ=255)
                                                    agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                 >  1:65735/1‑71 (MQ=255)
                                                     gtagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc                <  1:2796306/71‑1 (MQ=255)
                                                      tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGt                                                 >  1:1892548/1‑37 (MQ=255)
                                                      tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa               <  1:1573024/71‑1 (MQ=255)
                                                       agcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa               <  1:1378962/70‑1 (MQ=255)
                                                       agcgtagcCAGGCTACCAATTGGGGTAATTTTAGGTCCCAAATc                                         >  1:43043/1‑44 (MQ=255)
                                                        gcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                              <  1:2354634/54‑1 (MQ=255)
                                                        gcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc             >  1:2131489/1‑71 (MQ=255)
                                                         cgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc                               <  1:300475/52‑1 (MQ=255)
                                                             gcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGcc                                   <  1:807950/44‑1 (MQ=255)
                                                             gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCAtt             <  1:3134047/66‑2 (MQ=255)
                                                               cAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctctt      <  1:3613558/71‑1 (MQ=255)
                                                                aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                        <  1:2198133/36‑1 (MQ=255)
                                                                   cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa   >  1:3749195/1‑70 (MQ=255)
                                                                    tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt  >  1:1270227/1‑70 (MQ=255)
                                                                     |                                                                    
GTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAT  >  minE/2595392‑2595529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: