| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,473 | A→G | 14.4% | I375I (ATT→ATC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,473 | 0 | A | G | 14.4% | 220.9 / 26.4 | 90 | I375I (ATT→ATC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (36/41); new base G (8/5); total (44/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01 | |||||||||||
ATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCG > minE/2595408‑2595543 | aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt < 1:1167928/70‑1 (MQ=255)aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt < 1:1179279/70‑1 (MQ=255) tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:978495/1‑71 (MQ=255) tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc < 1:2374378/71‑1 (MQ=255) gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg < 1:3285187/71‑1 (MQ=255) atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTc < 1:695482/70‑1 (MQ=255) tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc > 1:3678810/1‑70 (MQ=255) atTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc > 1:769487/1‑70 (MQ=255) ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa < 1:2313497/71‑1 (MQ=255) cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa < 1:3880676/70‑1 (MQ=255) ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg < 1:814116/61‑1 (MQ=255) ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc > 1:3061286/1‑71 (MQ=255) tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:2638405/1‑59 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:2438638/70‑1 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:1535688/70‑1 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg > 1:2872330/1‑70 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:1361854/70‑1 (MQ=255) tGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg > 1:1985086/1‑69 (MQ=255) gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGACTACCAATTGGGGTAATTTTCGGTCCCAAATc < 1:3885812/67‑1 (MQ=255) gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt > 1:3457010/1‑51 (MQ=255) gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:469164/1‑70 (MQ=255) gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:3683788/1‑70 (MQ=255) aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:744154/1‑69 (MQ=255) aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:1781988/1‑69 (MQ=255) aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa > 1:1406051/1‑71 (MQ=255) aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt > 1:1530414/1‑49 (MQ=255) gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc < 1:1687020/71‑1 (MQ=255) aCGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca > 1:1281937/1‑70 (MQ=255) catGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcaca > 1:751980/3‑71 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg > 1:2788713/1‑48 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca > 1:1926630/1‑71 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:3130744/71‑1 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:272622/71‑1 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc > 1:1997930/1‑46 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCa < 1:114021/53‑1 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt < 1:3619878/71‑1 (MQ=255) tGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt < 1:2537102/71‑1 (MQ=255) gaaGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:3682967/3‑71 (MQ=255) gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:1279832/1‑71 (MQ=255) gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:328935/1‑71 (MQ=255) gCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg > 1:3239388/1‑71 (MQ=255) gCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg > 1:3045573/1‑71 (MQ=255) cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGTGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:1087227/70‑1 (MQ=255) cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:1043247/70‑1 (MQ=255) cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:831074/70‑1 (MQ=255) aGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:2735754/69‑1 (MQ=255) gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc < 1:3476446/52‑1 (MQ=255) ccAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCa > 1:2097381/1‑70 (MQ=255) cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa < 1:3680750/71‑1 (MQ=255) agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa > 1:927278/1‑70 (MQ=255) gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:2372881/61‑1 (MQ=255) gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt > 1:875123/1‑63 (MQ=255) gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt > 1:533963/1‑63 (MQ=255) agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaaa > 1:2420487/1‑70 (MQ=255) agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc > 1:65735/1‑71 (MQ=255) gtagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc < 1:2796306/71‑1 (MQ=255) tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGt > 1:1892548/1‑37 (MQ=255) tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa < 1:1573024/71‑1 (MQ=255) agcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa < 1:1378962/70‑1 (MQ=255) agcgtagcCAGGCTACCAATTGGGGTAATTTTAGGTCCCAAATc > 1:43043/1‑44 (MQ=255) gcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca < 1:2354634/54‑1 (MQ=255) gcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc > 1:2131489/1‑71 (MQ=255) cgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc < 1:300475/52‑1 (MQ=255) gcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGcc < 1:807950/44‑1 (MQ=255) gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCAtt < 1:3134047/66‑2 (MQ=255) cAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctctt < 1:3613558/71‑1 (MQ=255) aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:2198133/36‑1 (MQ=255) cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa > 1:3749195/1‑70 (MQ=255) tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt > 1:1270227/1‑70 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa < 1:3592426/70‑1 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa < 1:496167/70‑1 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc < 1:686166/71‑1 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc > 1:2744289/1‑71 (MQ=255) ccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGacga < 1:1759982/70‑4 (MQ=255) cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACg > 1:3597233/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCGATCACATTGGCATAAATCATTGCCTCTTTGACGACGc > 1:1067913/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc > 1:1142962/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:2056505/71‑1 (MQ=255) aTTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGcc < 1:448498/71‑1 (MQ=255) aTTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:1826369/1‑70 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCa < 1:966097/71‑1 (MQ=255) tcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc > 1:3146126/3‑69 (MQ=255) cggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGc > 1:3645109/2‑42 (MQ=25) cggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGc > 1:3469152/2‑42 (MQ=25) gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc < 1:1039489/71‑3 (MQ=255) gTAATTTTCTGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGc > 1:3629754/1‑71 (MQ=255) gTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc < 1:45937/48‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc < 1:3684269/44‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa < 1:963935/45‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:1716718/71‑1 (MQ=255) aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCa > 1:920003/1‑37 (MQ=255) aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg > 1:3548375/1‑71 (MQ=255) | ATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCG > minE/2595408‑2595543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |