| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,482 | T→G | 22.6% | G372G (GGA→GGC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,482 | 0 | T | G | 22.6% | 149.9 / 74.0 | 111 | G372G (GGA→GGC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (43/43); new base G (15/10); total (58/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
ATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCA > minE/2595414‑2595551 | atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTc < 1:695482/70‑1 (MQ=255) tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc > 1:3678810/1‑70 (MQ=255) atTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc > 1:769487/1‑70 (MQ=255) ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc > 1:3061286/1‑71 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:2438638/70‑1 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:1535688/70‑1 (MQ=255) ctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg > 1:2872330/1‑70 (MQ=255) tGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg > 1:1985086/1‑69 (MQ=255) gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGACTACCAATTGGGGTAATTTTCGGTCCCAAATc < 1:3885812/67‑1 (MQ=255) gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:469164/1‑70 (MQ=255) aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:1781988/1‑69 (MQ=255) aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa > 1:1406051/1‑71 (MQ=255) gTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc < 1:1687020/71‑1 (MQ=255) aCGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca > 1:1281937/1‑70 (MQ=255) catGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcaca > 1:751980/3‑71 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca > 1:1926630/1‑71 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:272622/71‑1 (MQ=255) cGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:3130744/71‑1 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCa < 1:114021/53‑1 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt < 1:3619878/71‑1 (MQ=255) gaaGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:3682967/3‑71 (MQ=255) gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:1279832/1‑71 (MQ=255) gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:328935/1‑71 (MQ=255) gCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg > 1:3239388/1‑71 (MQ=255) gCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg > 1:3045573/1‑71 (MQ=255) cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:831074/70‑1 (MQ=255) cAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:1043247/70‑1 (MQ=255) aGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg < 1:2735754/69‑1 (MQ=255) gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc < 1:3476446/52‑1 (MQ=255) cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa < 1:3680750/71‑1 (MQ=255) agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa > 1:927278/1‑70 (MQ=255) gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:2372881/61‑1 (MQ=255) gagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt > 1:533963/1‑63 (MQ=255) agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaaa > 1:2420487/1‑70 (MQ=255) agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc > 1:65735/1‑71 (MQ=255) gtagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc < 1:2796306/71‑1 (MQ=255) tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGt > 1:1892548/1‑37 (MQ=255) tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa < 1:1573024/71‑1 (MQ=255) agcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa < 1:1378962/70‑1 (MQ=255) agcgtagcCAGGCTACCAATTGGGGTAATTTTAGGTCCCAAATc > 1:43043/1‑44 (MQ=255) gcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca < 1:2354634/54‑1 (MQ=255) gcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc > 1:2131489/1‑71 (MQ=255) cgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc < 1:300475/52‑1 (MQ=255) gcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGcc < 1:807950/44‑1 (MQ=255) gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCAtt < 1:3134047/66‑2 (MQ=255) cAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctctt < 1:3613558/71‑1 (MQ=255) aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg < 1:2198133/36‑1 (MQ=255) cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa > 1:3749195/1‑70 (MQ=255) tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt > 1:1270227/1‑70 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa < 1:3592426/70‑1 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc > 1:2744289/1‑71 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc < 1:686166/71‑1 (MQ=255) ccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGacga < 1:1759982/70‑4 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCGATCACATTGGCATAAATCATTGCCTCTTTGACGACGc > 1:1067913/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc > 1:1142962/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:2056505/71‑1 (MQ=255) aTTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGcc < 1:448498/71‑1 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCa < 1:966097/71‑1 (MQ=255) tcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc > 1:3146126/3‑69 (MQ=255) cggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGc > 1:3469152/2‑42 (MQ=25) cggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGc > 1:3645109/2‑42 (MQ=25) gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc < 1:1039489/71‑3 (MQ=255) gTAATTTTCTGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGc > 1:3629754/1‑71 (MQ=255) gTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc < 1:45937/48‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc < 1:3684269/44‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa < 1:963935/45‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:1716718/71‑1 (MQ=255) gaTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg > 1:2310222/2‑71 (MQ=255) aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCa > 1:920003/1‑37 (MQ=255) aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg > 1:3548375/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc > 1:1823567/1‑45 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc > 1:3061579/1‑45 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAGGCCAGATGCCGt > 1:3657593/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg < 1:3725864/70‑1 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg < 1:3632583/70‑1 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt > 1:2282745/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCACCCAATCACATTGGCATAAACCATc > 1:3530414/1‑45 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCttt > 1:3659092/1‑53 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg < 1:373726/64‑1 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg < 1:2776004/64‑1 (MQ=255) ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGctt < 1:1153299/48‑1 (MQ=255) ttttCGGGCCTAAATCACAGCTAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta < 1:281000/71‑2 (MQ=255) ttttCGGGCCTAAATCACAGCCAATCACATTGGCATaaatc < 1:636744/41‑3 (MQ=255) tttCGGTCCCAAATCGCAGCCAATCACATTGGCATaa > 1:152663/1‑37 (MQ=255) ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:1318215/1‑71 (MQ=255) ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:1834918/1‑71 (MQ=255) tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg > 1:1079007/1‑71 (MQ=255) tCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc > 1:1335928/1‑70 (MQ=255) cGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc < 1:1014757/70‑2 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc > 1:1098596/1‑70 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc < 1:2603902/70‑1 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGcc < 1:2138816/71‑1 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3081292/71‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg < 1:803192/46‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg < 1:2384325/46‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg < 1:2643761/46‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa > 1:884215/1‑47 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCat < 1:3070565/56‑2 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg < 1:1221579/64‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg < 1:3699881/64‑1 (MQ=255) | ATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCA > minE/2595414‑2595551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |