| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,518 | C→T | 25.2% | A360A (GCG→GCA) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,518 | 0 | C | T | 25.2% | 223.8 / 78.5 | 127 | A360A (GCG→GCA) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (51/44); new base T (20/12); total (71/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATA > minE/2595448‑2595587 | gcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc > 1:2131489/1‑71 (MQ=255) cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa > 1:3749195/1‑70 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa < 1:496167/70‑1 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa < 1:3592426/70‑1 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc < 1:686166/71‑1 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc > 1:2744289/1‑71 (MQ=255) ccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGacga < 1:1759982/70‑4 (MQ=255) cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACg > 1:3597233/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc > 1:1142962/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:2056505/71‑1 (MQ=255) aTTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGcc < 1:448498/71‑1 (MQ=255) aTTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:1826369/1‑70 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCa < 1:966097/71‑1 (MQ=255) gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc < 1:1039489/71‑3 (MQ=255) gTAATTTTCTGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGc > 1:3629754/1‑71 (MQ=255) gTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc < 1:45937/48‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:1716718/71‑1 (MQ=255) gaTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg > 1:2310222/2‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc > 1:3061579/1‑45 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc > 1:3104766/1‑45 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAGGCCAGATGCCGt > 1:3657593/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg < 1:3632583/70‑1 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt > 1:2282745/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt > 1:3045419/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCACCCAATCACATTGGCATAAACCATc > 1:3530414/1‑45 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCttt > 1:3659092/1‑53 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCttt > 1:1575010/1‑53 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg < 1:2776004/64‑1 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg < 1:373726/64‑1 (MQ=255) ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGctt < 1:1153299/48‑1 (MQ=255) ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:1318215/1‑71 (MQ=255) ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:1834918/1‑71 (MQ=255) tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg > 1:1079007/1‑71 (MQ=255) tCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg > 1:1243003/1‑60 (MQ=255) tCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc > 1:1335928/1‑70 (MQ=255) cGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcct > 1:3654912/1‑42 (MQ=255) cGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc < 1:1014757/70‑2 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc > 1:1098596/1‑70 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGcc < 1:2138816/71‑1 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3081292/71‑1 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3379826/71‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg < 1:803192/46‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg < 1:2643761/46‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa > 1:884215/1‑47 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg < 1:1221579/64‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg < 1:3699881/64‑1 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg > 1:3733789/1‑66 (MQ=255) cccAAATCGAAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:556/1‑70 (MQ=255) cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGAt > 1:1126735/1‑55 (MQ=255) cAAATCGCAGCCAATCACATTGGCATAAAACATCGCTTCTTTGATaa > 1:1108861/1‑47 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGctt > 1:3549646/1‑37 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt > 1:2797251/1‑71 (MQ=255) aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgt > 1:609924/1‑52 (MQ=255) aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgt > 1:2970104/1‑52 (MQ=255) aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt > 1:3493069/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:96736/1‑60 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:3524820/1‑60 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2405114/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2105044/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:899769/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1132875/1‑71 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta > 1:743546/1‑58 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc > 1:1814704/1‑70 (MQ=255) aTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa > 1:3412466/1‑64 (MQ=255) aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa < 1:3923911/68‑1 (MQ=255) cGCAGCCAATCACATTGGCATAAACCATCGGTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:810901/1‑70 (MQ=255) cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa < 1:744751/71‑1 (MQ=255) aGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACagc > 1:2383520/1‑69 (MQ=255) aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc < 1:1588070/71‑1 (MQ=255) gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg < 1:925202/71‑1 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAg > 1:200458/1‑43 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg > 1:2386721/1‑70 (MQ=255) cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:3797257/1‑70 (MQ=255) cAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:1894529/70‑1 (MQ=255) aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca < 1:2834251/70‑2 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:3800795/57‑1 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:3837660/1‑71 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc < 1:301922/71‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:794708/45‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg > 1:3140959/1‑46 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt > 1:2633605/1‑47 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:1290411/54‑1 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCTAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:3386963/1‑70 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2580474/1‑59 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:2944604/1‑70 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc < 1:3891851/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:1891077/71‑1 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa < 1:2081315/71‑1 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:1018982/71‑1 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:2753474/1‑70 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:283908/1‑70 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:331902/1‑70 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3172012/47‑1 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:778934/57‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc > 1:2829819/1‑56 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg < 1:1832053/70‑1 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:3243634/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc < 1:62314/70‑1 (MQ=255) tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata > 1:1873291/1‑71 (MQ=255) cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:1589313/48‑1 (MQ=255) | GCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATA > minE/2595448‑2595587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |