| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,529 | T→C | 21.6% | I357V (ATC→GTC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,529 | 0 | T | C | 21.6% | 263.2 / 71.1 | 134 | I357V (ATC→GTC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (52/53); new base C (19/10); total (71/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 | |||||||||||
Only 100 of 137 total aligned reads displayed.
TACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATA > minE/2595460‑2595599 | tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt > 1:1270227/1‑70 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa < 1:496167/70‑1 (MQ=255) cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACg > 1:3597233/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCGATCACATTGGCATAAATCATTGCCTCTTTGACGACGc > 1:1067913/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:2056505/71‑1 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCa < 1:966097/71‑1 (MQ=255) gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc < 1:1039489/71‑3 (MQ=255) gTAATTTTCTGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGc > 1:3629754/1‑71 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:1716718/71‑1 (MQ=255) aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg > 1:3548375/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAGGCCAGATGCCGt > 1:3657593/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt > 1:2282745/1‑71 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg < 1:373726/64‑1 (MQ=255) ttttCGGGCCTAAATCACAGCTAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta < 1:281000/71‑2 (MQ=255) ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:1834918/1‑71 (MQ=255) tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg > 1:1079007/1‑71 (MQ=255) tCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc > 1:1335928/1‑70 (MQ=255) cGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc < 1:1014757/70‑2 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc > 1:1098596/1‑70 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc < 1:2603902/70‑1 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGcc < 1:2138816/71‑1 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3081292/71‑1 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3379826/71‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCat < 1:3070565/56‑2 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAg < 1:2746220/56‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg < 1:3699881/64‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg < 1:1221579/64‑1 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg > 1:3733789/1‑66 (MQ=255) cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGAt > 1:1126735/1‑55 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt > 1:2797251/1‑71 (MQ=255) aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgt > 1:609924/1‑52 (MQ=255) aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgt > 1:2970104/1‑52 (MQ=255) aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt > 1:3493069/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:96736/1‑60 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:3524820/1‑60 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:2865012/71‑1 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2405114/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1132875/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2105044/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:2090554/71‑1 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1919377/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:899769/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1673561/1‑71 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta > 1:743546/1‑58 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc > 1:1814704/1‑70 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc > 1:453421/1‑70 (MQ=255) aTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa > 1:3412466/1‑64 (MQ=255) aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa < 1:3923911/68‑1 (MQ=255) aGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACagc > 1:2383520/1‑69 (MQ=255) aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc < 1:1588070/71‑1 (MQ=255) gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg < 1:925202/71‑1 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAg > 1:200458/1‑43 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCa < 1:1114362/60‑1 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg > 1:2386721/1‑70 (MQ=255) gaaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAGCAATGGACAAcgcg < 1:1149530/70‑1 (MQ=255) cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:3797257/1‑70 (MQ=255) aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgcac > 1:782482/1‑69 (MQ=255) aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca < 1:2834251/70‑2 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:3800795/57‑1 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:3837660/1‑71 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc < 1:301922/71‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:794708/45‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg > 1:3140959/1‑46 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt > 1:2633605/1‑47 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:1290411/54‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:2994059/1‑70 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:729627/1‑70 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:2944604/1‑70 (MQ=255) acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaat > 1:831528/1‑70 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc < 1:3891851/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:573784/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:1891077/71‑1 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:1018982/71‑1 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:3657448/71‑1 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:2753474/1‑70 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:283908/1‑70 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3334613/47‑1 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3172012/47‑1 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:778934/57‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg < 1:1832053/70‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:3470445/1‑71 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:3243634/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc < 1:62314/70‑1 (MQ=255) tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata > 1:1873291/1‑71 (MQ=255) cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:1589313/48‑1 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:1827212/1‑55 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat < 1:1738194/70‑1 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:1389065/1‑71 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt < 1:1414431/71‑1 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:2641752/71‑1 (MQ=255) ttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc < 1:280419/57‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt < 1:3518380/70‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt < 1:1413438/69‑1 (MQ=255) tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCa > 1:1299074/1‑71 (MQ=255) gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata < 1:2228124/70‑1 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataat < 1:501691/71‑1 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat > 1:1404703/1‑68 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat > 1:3220389/1‑68 (MQ=255) tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata < 1:3771082/71‑1 (MQ=255) tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:3037491/1‑69 (MQ=255) | TACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATA > minE/2595460‑2595599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |