| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,538 | A→T | 16.5% | S354T (TCT→ACT) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,538 | 0 | A | T | 16.5% | 302.9 / 47.2 | 121 | S354T (TCT→ACT) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (53/48); new base T (13/7); total (66/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 121 total aligned reads displayed.
GTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGA > minE/2595471‑2595606 | gTAATTTTCTGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGc > 1:3629754/1‑71 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:1716718/71‑1 (MQ=255) gaTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg > 1:2310222/2‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAGGCCAGATGCCGt > 1:3657593/1‑71 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg < 1:3632583/70‑1 (MQ=255) aTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt > 1:2282745/1‑71 (MQ=255) ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:1318215/1‑71 (MQ=255) ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:1834918/1‑71 (MQ=255) tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg > 1:1079007/1‑71 (MQ=255) tCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc > 1:1335928/1‑70 (MQ=255) cGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc < 1:1014757/70‑2 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctgc > 1:1098596/1‑70 (MQ=255) ggTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGcc < 1:2138816/71‑1 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3379826/71‑1 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3081292/71‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg < 1:1221579/64‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtg < 1:3699881/64‑1 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgctg > 1:3733789/1‑66 (MQ=255) cccAAATCGAAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:556/1‑70 (MQ=255) cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGAt > 1:1126735/1‑55 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt > 1:2797251/1‑71 (MQ=255) aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt > 1:3493069/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:3524820/1‑60 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:96736/1‑60 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:2865012/71‑1 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:2090554/71‑1 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2405114/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1919377/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:899769/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1132875/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1673561/1‑71 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta > 1:743546/1‑58 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc > 1:453421/1‑70 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc > 1:1814704/1‑70 (MQ=255) aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa < 1:3923911/68‑1 (MQ=255) cGCAGCCAATCACATTGGCATAAACCATCGGTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:810901/1‑70 (MQ=255) cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa < 1:2174292/71‑1 (MQ=255) aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc < 1:1588070/71‑1 (MQ=255) gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg < 1:925202/71‑1 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg > 1:2386721/1‑70 (MQ=255) gaaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAGCAATGGACAAcgcg < 1:1149530/70‑1 (MQ=255) cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:3797257/1‑70 (MQ=255) cAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:1894529/70‑1 (MQ=255) aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgcac > 1:782482/1‑69 (MQ=255) aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca < 1:2834251/70‑2 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc < 1:301922/71‑1 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:3837660/1‑71 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:794708/45‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg > 1:3140959/1‑46 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt > 1:2633605/1‑47 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:1290411/54‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:2994059/1‑70 (MQ=255) taacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:377381/3‑59 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCTAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:3386963/1‑70 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2580474/1‑59 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:2944604/1‑70 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:729627/1‑70 (MQ=255) acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaat > 1:831528/1‑70 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc < 1:3891851/71‑1 (MQ=255) caTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaatc < 1:960083/71‑3 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:1891077/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:573784/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:3866880/71‑1 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa < 1:2081315/71‑1 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:1018982/71‑1 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:3657448/71‑1 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:331902/1‑70 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:2753474/1‑70 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:283908/1‑70 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3334613/47‑1 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3172012/47‑1 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:778934/57‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc > 1:2829819/1‑56 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg < 1:1832053/70‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:3470445/1‑71 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:3243634/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc < 1:62314/70‑1 (MQ=255) aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc > 1:1860656/1‑56 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:1827212/1‑55 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat < 1:1697944/70‑1 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:1389065/1‑71 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt < 1:1414431/71‑1 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg < 1:349717/47‑1 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg > 1:1063083/1‑71 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:2641752/71‑1 (MQ=255) ttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc < 1:280419/57‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt < 1:3518380/70‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt < 1:1413438/69‑1 (MQ=255) cttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt > 1:3001570/1‑70 (MQ=255) tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa < 1:1517361/69‑1 (MQ=255) gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata < 1:2228124/70‑1 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat > 1:1404703/1‑68 (MQ=255) tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata < 1:3771082/71‑1 (MQ=255) tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:3037491/1‑69 (MQ=255) aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc > 1:1638895/1‑49 (MQ=255) ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:2593501/1‑40 (MQ=255) cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa > 1:1563804/1‑68 (MQ=255) aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:1388102/1‑69 (MQ=255) aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga < 1:3061942/71‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTc > 1:2859084/1‑57 (MQ=255) | GTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGA > minE/2595471‑2595606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |