| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,551 | A→G | 16.3% | D349D (GAT→GAC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,551 | 0 | A | G | 16.3% | 228.4 / 34.3 | 98 | D349D (GAT→GAC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (37/45); new base G (9/7); total (46/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGG > minE/2595481‑2595620 | gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3081292/71‑1 (MQ=255)gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:3379826/71‑1 (MQ=255) cccAAATCGAAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:556/1‑70 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt > 1:2797251/1‑71 (MQ=255) aaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt > 1:3493069/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1919377/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1673561/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2405114/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:2865012/71‑1 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:899769/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:1132875/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2105044/1‑71 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc > 1:453421/1‑70 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc > 1:1814704/1‑70 (MQ=255) aTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa > 1:3412466/1‑64 (MQ=255) aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa < 1:3923911/68‑1 (MQ=255) cGCAGCCAATCACATTGGCATAAACCATCGGTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:810901/1‑70 (MQ=255) cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa < 1:744751/71‑1 (MQ=255) cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa < 1:2174292/71‑1 (MQ=255) aGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACagc > 1:2383520/1‑69 (MQ=255) aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc < 1:1588070/71‑1 (MQ=255) gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg < 1:925202/71‑1 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCa < 1:1114362/60‑1 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg > 1:2386721/1‑70 (MQ=255) gaaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAGCAATGGACAAcgcg < 1:1149530/70‑1 (MQ=255) cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:3797257/1‑70 (MQ=255) cAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:1894529/70‑1 (MQ=255) aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgcac > 1:782482/1‑69 (MQ=255) aaTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca < 1:2834251/70‑2 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:3800795/57‑1 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:3837660/1‑71 (MQ=255) aaTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc < 1:301922/71‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCa < 1:1290411/54‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:2994059/1‑70 (MQ=255) taacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:377381/3‑59 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCTAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:3386963/1‑70 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg > 1:2580474/1‑59 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:729627/1‑70 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc > 1:2944604/1‑70 (MQ=255) acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCaat > 1:831528/1‑70 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc < 1:3891851/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:1891077/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:3866880/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:573784/71‑1 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa < 1:2081315/71‑1 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:1018982/71‑1 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:3657448/71‑1 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:283908/1‑70 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg > 1:2753474/1‑70 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3334613/47‑1 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3172012/47‑1 (MQ=255) aTAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:778934/57‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc > 1:2829819/1‑56 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg < 1:1832053/70‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:3470445/1‑71 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:3243634/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc < 1:62314/70‑1 (MQ=255) aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc > 1:1860656/1‑56 (MQ=255) tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata > 1:1873291/1‑71 (MQ=255) cGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:1589313/48‑1 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:1827212/1‑55 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat < 1:1697944/70‑1 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat < 1:1738194/70‑1 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:1389065/1‑71 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt < 1:1414431/71‑1 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg < 1:349717/47‑1 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg > 1:1063083/1‑71 (MQ=255) cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:2641752/71‑1 (MQ=255) ttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc < 1:280419/57‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt < 1:3518380/70‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt < 1:2901691/69‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt < 1:1413438/69‑1 (MQ=255) cttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt > 1:3001570/1‑70 (MQ=255) tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCa > 1:1299074/1‑71 (MQ=255) tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa < 1:1517361/69‑1 (MQ=255) gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata < 1:2228124/70‑1 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataat < 1:501691/71‑1 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat > 1:1404703/1‑68 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat > 1:3220389/1‑68 (MQ=255) tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata < 1:3771082/71‑1 (MQ=255) tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:3037491/1‑69 (MQ=255) aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc > 1:1638895/1‑49 (MQ=255) aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:888457/70‑1 (MQ=255) ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:2593501/1‑40 (MQ=255) cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa > 1:1563804/1‑68 (MQ=255) aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:1388102/1‑69 (MQ=255) aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga < 1:3061942/71‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTc > 1:2859084/1‑57 (MQ=255) tcgCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATAtt < 1:2355834/50‑1 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa < 1:50266/71‑1 (MQ=255) ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa < 1:3224139/70‑1 (MQ=255) cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg < 1:2904511/71‑3 (MQ=255) cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg < 1:201724/71‑3 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1307564/69‑1 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:2708672/69‑1 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1413277/69‑1 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1184608/69‑1 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa < 1:1579238/70‑1 (MQ=255) cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg > 1:3614976/1‑70 (MQ=255) aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg > 1:2517067/1‑70 (MQ=255) | GTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGG > minE/2595481‑2595620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |