Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,571 C→T 16.0% V343I (GTT→ATT)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5710CT16.0% 196.6 / 25.5 81V343I (GTT→ATT) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (30/38);  new base T (4/9);  total (34/47)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCG  >  minE/2595501‑2595641
                                                                      |                                                                      
acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTTCTGCCATCAATGGACAACGCGCCAAc                                                                        <  1:3891851/71‑1 (MQ=255)
 caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                                                       <  1:1891077/71‑1 (MQ=255)
 caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                                                       <  1:3866880/71‑1 (MQ=255)
 caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                                                       <  1:573784/71‑1 (MQ=255)
  aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                                      <  1:2081315/71‑1 (MQ=255)
   ttGGCATAAACCATCGCTTCTTTGATATCGCCAGTTGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                                     <  1:1018982/71‑1 (MQ=255)
   ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                                     <  1:3657448/71‑1 (MQ=255)
    tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                                     >  1:2753474/1‑70 (MQ=255)
    tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                                     >  1:283908/1‑70 (MQ=255)
    tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                                     >  1:331902/1‑70 (MQ=255)
         tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg                                                                <  1:1832053/70‑1 (MQ=255)
         tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                               >  1:3470445/1‑71 (MQ=255)
          aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                              >  1:3243634/1‑71 (MQ=255)
           aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                              <  1:62314/70‑1 (MQ=255)
               aTCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                                        >  1:1860656/1‑56 (MQ=255)
                tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata                                                        >  1:1873291/1‑71 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                                      >  1:1827212/1‑55 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                                                       <  1:1697944/70‑1 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                                                       <  1:1738194/70‑1 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                                      <  1:1414431/71‑1 (MQ=255)
                  gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                                      >  1:1389065/1‑71 (MQ=255)
                   cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                     <  1:2641752/71‑1 (MQ=255)
                   cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                     >  1:1063083/1‑71 (MQ=255)
                    ttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                                  <  1:280419/57‑1 (MQ=255)
                      cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                                                   <  1:3518380/70‑1 (MQ=255)
                      cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                                    <  1:1413438/69‑1 (MQ=255)
                      cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                                    <  1:2901691/69‑1 (MQ=255)
                      cttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt                                                   >  1:3001570/1‑70 (MQ=255)
                       tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCa                                                 >  1:1299074/1‑71 (MQ=255)
                         tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa                                                 <  1:1517361/69‑1 (MQ=255)
                          gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                               <  1:2228124/70‑1 (MQ=255)
                           aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataat                                             <  1:501691/71‑1 (MQ=255)
                           aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                                >  1:3220389/1‑68 (MQ=255)
                           aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                                >  1:1404703/1‑68 (MQ=255)
                            tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata                                            <  1:3771082/71‑1 (MQ=255)
                            tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                              >  1:3037491/1‑69 (MQ=255)
                             aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                                 >  1:1638895/1‑49 (MQ=255)
                              aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                           <  1:888457/70‑1 (MQ=255)
                                 ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                                      >  1:2593501/1‑40 (MQ=255)
                                  cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                         >  1:1563804/1‑68 (MQ=255)
                                   aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       >  1:1388102/1‑69 (MQ=255)
                                   aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga                                     <  1:3061942/71‑1 (MQ=255)
                                    gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTc                                                  >  1:2859084/1‑57 (MQ=255)
                                     tcgCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATAtt                                                      <  1:2355834/50‑1 (MQ=255)
                                      tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa                                  <  1:50266/71‑1 (MQ=255)
                                        ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                                 <  1:3224139/70‑1 (MQ=255)
                                         cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                               <  1:2904511/71‑3 (MQ=255)
                                         cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                               <  1:201724/71‑3 (MQ=255)
                                                ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                          <  1:1184608/69‑1 (MQ=255)
                                                ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                          <  1:1307564/69‑1 (MQ=255)
                                                ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                          <  1:2708672/69‑1 (MQ=255)
                                                ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                          <  1:1413277/69‑1 (MQ=255)
                                                ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa                         <  1:1579238/70‑1 (MQ=255)
                                                 cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                        >  1:3614976/1‑70 (MQ=255)
                                                  aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg                       >  1:2517067/1‑70 (MQ=255)
                                                   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                     <  1:2784911/71‑1 (MQ=255)
                                                   tCAATCGACAGCGCACCAATCAGCACCGTCGGCat                                                         <  1:2375608/35‑1 (MQ=12)
                                                   tCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaa                                 >  1:493222/1‑59 (MQ=255)
                                                   tCAATCGACAGCGAACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGc                              >  1:3547802/1‑62 (MQ=255)
                                                    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa                         >  1:454727/1‑66 (MQ=255)
                                                    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                    >  1:1365583/1‑71 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga                                     >  1:2228250/1‑53 (MQ=255)
                                                     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                   >  1:2062411/1‑71 (MQ=255)
                                                     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaag                    <  1:1858041/70‑2 (MQ=255)
                                                     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGACGAGAGAAATGCCGTCAAGaagc                   <  1:1880062/71‑3 (MQ=255)
                                                      aTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATcc                  >  1:2230069/1‑71 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       <  1:2067647/49‑1 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAgg                                   <  1:1229662/53‑1 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg                 <  1:3703989/71‑1 (MQ=255)
                                                       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg                 >  1:3476805/1‑71 (MQ=255)
                                                         gACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt               <  1:1177483/71‑1 (MQ=255)
                                                         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGTCGGTGAGGAATCCGGt               <  1:2611145/71‑1 (MQ=255)
                                                         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                <  1:817798/70‑1 (MQ=255)
                                                          aCAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                                >  1:1218260/1‑37 (MQ=255)
                                                          aCAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       <  1:1834568/46‑1 (MQ=255)
                                                           cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                              <  1:3682830/38‑1 (MQ=255)
                                                             gcgcaCCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg           <  1:2432020/70‑1 (MQ=255)
                                                              cgcgCCAACCAGTACCGTCGGCATATTGTTCataa                                              <  1:2287436/35‑1 (MQ=255)
                                                               gcgcCAACCAGTACCGTCGGCATATTGTTCataat                                             <  1:3199146/35‑1 (MQ=255)
                                                               gcgcCAACCAGTACCGTCGGCATATTGTTCataat                                             <  1:860889/35‑1 (MQ=255)
                                                                   cAATCAGCACCGTCGGCATATTATTCATCACTGAtgagaga                                   <  1:1349700/41‑2 (MQ=255)
                                                                   cAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       >  1:1452556/1‑37 (MQ=255)
                                                                   cAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       >  1:3394895/1‑37 (MQ=255)
                                                                    aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                        >  1:2587318/1‑51 (MQ=255)
                                                                    aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                        >  1:773494/1‑51 (MQ=255)
                                                                     aCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgc   <  1:317021/71‑1 (MQ=255)
                                                                      ccAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgcg  >  1:185886/1‑71 (MQ=255)
                                                                      |                                                                      
ACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCG  >  minE/2595501‑2595641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: