Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,575 T→C 18.7% V341V (GTA→GTG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,5750TC18.7% 162.7 / 30.3 75V341V (GTA→GTGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (26/35);  new base C (5/9);  total (31/44)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCG  >  minE/2595510‑2595641
                                                                 |                                                                  
tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCg                                                                <  1:1832053/70‑1 (MQ=255)
tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                               >  1:3470445/1‑71 (MQ=255)
 aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                              >  1:3243634/1‑71 (MQ=255)
  aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                              <  1:62314/70‑1 (MQ=255)
       tCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCata                                                        >  1:1873291/1‑71 (MQ=255)
         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                                                       <  1:1738194/70‑1 (MQ=255)
         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCatat                                                       <  1:1697944/70‑1 (MQ=255)
         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                                      <  1:1414431/71‑1 (MQ=255)
         gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                                      >  1:1389065/1‑71 (MQ=255)
          cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                     <  1:2641752/71‑1 (MQ=255)
          cttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                     >  1:1063083/1‑71 (MQ=255)
           ttcttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                                  <  1:280419/57‑1 (MQ=255)
             cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                                                   <  1:3518380/70‑1 (MQ=255)
             cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                                    <  1:1413438/69‑1 (MQ=255)
             cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                                    <  1:2901691/69‑1 (MQ=255)
             cttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt                                                   >  1:3001570/1‑70 (MQ=255)
              tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCa                                                 >  1:1299074/1‑71 (MQ=255)
                tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCa                                                 <  1:1517361/69‑1 (MQ=255)
                 gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                               <  1:2228124/70‑1 (MQ=255)
                  aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataat                                             <  1:501691/71‑1 (MQ=255)
                  aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                                >  1:1404703/1‑68 (MQ=255)
                  aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                                >  1:3220389/1‑68 (MQ=255)
                   tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata                                            <  1:3771082/71‑1 (MQ=255)
                   tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                              >  1:3037491/1‑69 (MQ=255)
                    aaCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                                 >  1:1638895/1‑49 (MQ=255)
                     aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                           <  1:888457/70‑1 (MQ=255)
                         cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                         >  1:1563804/1‑68 (MQ=255)
                          aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       >  1:1388102/1‑69 (MQ=255)
                          aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga                                     <  1:3061942/71‑1 (MQ=255)
                           gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTc                                                  >  1:2859084/1‑57 (MQ=255)
                            tcgCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATAtt                                                      <  1:2355834/50‑1 (MQ=255)
                             tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa                                  <  1:50266/71‑1 (MQ=255)
                               ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                                 <  1:3224139/70‑1 (MQ=255)
                                cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                               <  1:201724/71‑3 (MQ=255)
                                cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                               <  1:2904511/71‑3 (MQ=255)
                                       ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                          <  1:1307564/69‑1 (MQ=255)
                                       ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                          <  1:2708672/69‑1 (MQ=255)
                                       ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                          <  1:1184608/69‑1 (MQ=255)
                                       ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                          <  1:1413277/69‑1 (MQ=255)
                                       ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa                         <  1:1579238/70‑1 (MQ=255)
                                        cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                        >  1:3614976/1‑70 (MQ=255)
                                         aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg                       >  1:2517067/1‑70 (MQ=255)
                                          tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                     <  1:2784911/71‑1 (MQ=255)
                                          tCAATCGACAGCGCACCAATCAGCACCGTCGGCat                                                         <  1:2375608/35‑1 (MQ=12)
                                          tCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaa                                 >  1:493222/1‑59 (MQ=255)
                                          tCAATCGACAGCGAACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGc                              >  1:3547802/1‑62 (MQ=255)
                                           cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa                         >  1:454727/1‑66 (MQ=255)
                                           cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                    >  1:1365583/1‑71 (MQ=255)
                                            aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga                                     >  1:2228250/1‑53 (MQ=255)
                                            aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                   >  1:2062411/1‑71 (MQ=255)
                                            aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaag                    <  1:1858041/70‑2 (MQ=255)
                                            aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGACGAGAGAAATGCCGTCAAGaagc                   <  1:1880062/71‑3 (MQ=255)
                                             aTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATcc                  >  1:2230069/1‑71 (MQ=255)
                                              tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       <  1:2067647/49‑1 (MQ=255)
                                              tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAgg                                   <  1:1229662/53‑1 (MQ=255)
                                              tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg                 <  1:3703989/71‑1 (MQ=255)
                                              tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg                 >  1:3476805/1‑71 (MQ=255)
                                                gACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt               <  1:1177483/71‑1 (MQ=255)
                                                gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGTCGGTGAGGAATCCGGt               <  1:2611145/71‑1 (MQ=255)
                                                gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                <  1:817798/70‑1 (MQ=255)
                                                 aCAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                                >  1:1218260/1‑37 (MQ=255)
                                                 aCAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       <  1:1834568/46‑1 (MQ=255)
                                                  cAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                              <  1:3682830/38‑1 (MQ=255)
                                                    gcgcaCCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg           <  1:2432020/70‑1 (MQ=255)
                                                     cgcgCCAACCAGTACCGTCGGCATATTGTTCataa                                              <  1:2287436/35‑1 (MQ=255)
                                                      gcgcCAACCAGTACCGTCGGCATATTGTTCataat                                             <  1:3199146/35‑1 (MQ=255)
                                                      gcgcCAACCAGTACCGTCGGCATATTGTTCataat                                             <  1:860889/35‑1 (MQ=255)
                                                          cAATCAGCACCGTCGGCATATTATTCATCACTGAtgagaga                                   <  1:1349700/41‑2 (MQ=255)
                                                          cAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       >  1:1452556/1‑37 (MQ=255)
                                                          cAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                       >  1:3394895/1‑37 (MQ=255)
                                                           aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                        >  1:2587318/1‑51 (MQ=255)
                                                           aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                        >  1:773494/1‑51 (MQ=255)
                                                            aCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgc   <  1:317021/71‑1 (MQ=255)
                                                             tcAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcgat  >  1:1449819/2‑69 (MQ=255)
                                                             ccAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgcg  >  1:185886/1‑71 (MQ=255)
                                                                 |                                                                  
TAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCG  >  minE/2595510‑2595641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: