| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,599 | A→T | 25.5% | S333S (TCT→TCA) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,599 | 0 | A | T | 25.5% | 80.5 / 29.8 | 47 | S333S (TCT→TCA) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (12/23); new base T (3/9); total (15/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.32e-01 | |||||||||||
TAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACG > minE/2595529‑2595668 | tAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataata < 1:3771082/71‑1 (MQ=255) aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:888457/70‑1 (MQ=255) cAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa > 1:1563804/1‑68 (MQ=255) aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:1388102/1‑69 (MQ=255) aGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga < 1:3061942/71‑1 (MQ=255) tGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGa < 1:50266/71‑1 (MQ=255) ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa < 1:3224139/70‑1 (MQ=255) cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg < 1:201724/71‑3 (MQ=255) cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg < 1:2904511/71‑3 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1413277/69‑1 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:2708672/69‑1 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1307564/69‑1 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg < 1:1184608/69‑1 (MQ=255) ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa < 1:1579238/70‑1 (MQ=255) cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg > 1:3614976/1‑70 (MQ=255) aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg > 1:2517067/1‑70 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa < 1:2784911/71‑1 (MQ=255) tCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaa > 1:493222/1‑59 (MQ=255) tCAATCGACAGCGAACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGc > 1:3547802/1‑62 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa > 1:454727/1‑66 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt > 1:1365583/1‑71 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagaga > 1:2228250/1‑53 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:2062411/1‑71 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaag < 1:1858041/70‑2 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGACGAGAGAAATGCCGTCAAGaagc < 1:1880062/71‑3 (MQ=255) aTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATcc > 1:2230069/1‑71 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:2067647/49‑1 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAgg < 1:1229662/53‑1 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg > 1:3476805/1‑71 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg < 1:3703989/71‑1 (MQ=255) gACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt < 1:1177483/71‑1 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGTCGGTGAGGAATCCGGt < 1:2611145/71‑1 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg < 1:817798/70‑1 (MQ=255) aCAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:1834568/46‑1 (MQ=255) gcgcaCCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:2432020/70‑1 (MQ=255) cAATCAGCACCGTCGGCATATTATTCATCACTGAtgagaga < 1:1349700/41‑2 (MQ=255) cAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:1452556/1‑37 (MQ=255) cAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:3394895/1‑37 (MQ=255) aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg > 1:2587318/1‑51 (MQ=255) aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg > 1:773494/1‑51 (MQ=255) aCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgc < 1:317021/71‑1 (MQ=255) tcAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcgat > 1:1449819/2‑69 (MQ=255) ccAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgcg > 1:185886/1‑71 (MQ=255) agcaCCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc < 1:3215003/68‑1 (MQ=255) aCCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCca < 1:2235688/71‑1 (MQ=255) cGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCcac < 1:1424282/70‑1 (MQ=255) gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg < 1:3928906/71‑1 (MQ=255) tCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGa < 1:249413/54‑1 (MQ=255) tCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCcaca < 1:1126770/69‑1 (MQ=255) ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:536063/1‑43 (MQ=255) ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc < 1:2856962/71‑1 (MQ=255) gCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGcc < 1:3830264/49‑1 (MQ=255) atatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgcg < 1:3333826/57‑1 (MQ=255) tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt < 1:3353065/43‑1 (MQ=255) gTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATcc < 1:1177307/70‑1 (MQ=255) ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccccc > 1:810155/1‑49 (MQ=255) taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:1827776/71‑1 (MQ=255) taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:3353876/71‑1 (MQ=255) taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:3340122/71‑1 (MQ=255) | TAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACG > minE/2595529‑2595668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |