Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,611 G→T 17.0% A329A (GCC→GCA arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,6110GT17.0% 127.8 / 10.2 47A329A (GCC→GCAarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (12/27);  new base T (2/6);  total (14/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

CCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAG  >  minE/2595549‑2595681
                                                              |                                                                      
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                                                                  <  1:2708672/69‑1 (MQ=255)
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                                                                  <  1:1184608/69‑1 (MQ=255)
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                                                                  <  1:1307564/69‑1 (MQ=255)
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTg                                                                  <  1:1413277/69‑1 (MQ=255)
ccATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa                                                                 <  1:1579238/70‑1 (MQ=255)
 cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                                                                >  1:3614976/1‑70 (MQ=255)
  aTCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAgg                                                               >  1:2517067/1‑70 (MQ=255)
   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                                             <  1:2784911/71‑1 (MQ=255)
   tCAATCGACAGCGAACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGc                                                                      >  1:3547802/1‑62 (MQ=255)
    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGa                                                                 >  1:454727/1‑66 (MQ=255)
    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                                                            >  1:1365583/1‑71 (MQ=255)
     aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                                                           >  1:2062411/1‑71 (MQ=255)
     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaag                                                            <  1:1858041/70‑2 (MQ=255)
     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGACGAGAGAAATGCCGTCAAGaagc                                                           <  1:1880062/71‑3 (MQ=255)
      aTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATcc                                                          >  1:2230069/1‑71 (MQ=255)
       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg                                                         <  1:3703989/71‑1 (MQ=255)
       tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCg                                                         >  1:3476805/1‑71 (MQ=255)
         gACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt                                                       <  1:1177483/71‑1 (MQ=255)
         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGTCGGTGAGGAATCCGGt                                                       <  1:2611145/71‑1 (MQ=255)
         gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                        <  1:817798/70‑1 (MQ=255)
             gcgcaCCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                                   <  1:2432020/70‑1 (MQ=255)
                    aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                                                                >  1:773494/1‑51 (MQ=255)
                    aaCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAg                                                                >  1:2587318/1‑51 (MQ=255)
                     aCCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgc                                           <  1:317021/71‑1 (MQ=255)
                      tcAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcgat                                          >  1:1449819/2‑69 (MQ=255)
                      ccAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgcg                                          >  1:185886/1‑71 (MQ=255)
                        agcaCCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc                                        <  1:3215003/68‑1 (MQ=255)
                           aCCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCca                                     <  1:2235688/71‑1 (MQ=255)
                             cGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCcac                                    <  1:1424282/70‑1 (MQ=255)
                              gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg                                  <  1:3928906/71‑1 (MQ=255)
                               tCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGa                                                  <  1:249413/54‑1 (MQ=255)
                               tCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCcaca                                   <  1:1126770/69‑1 (MQ=255)
                                 ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                                                           >  1:536063/1‑43 (MQ=255)
                                 ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc                               <  1:2856962/71‑1 (MQ=255)
                                  gCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGcc                                                    <  1:3830264/49‑1 (MQ=255)
                                    atatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTcgcg                                          <  1:3333826/57‑1 (MQ=255)
                                     tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGt                                                       <  1:3353065/43‑1 (MQ=255)
                                         gTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATcc                        <  1:1177307/70‑1 (MQ=255)
                                                ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccccc                                    >  1:810155/1‑49 (MQ=255)
                                                 taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg               <  1:3353876/71‑1 (MQ=255)
                                                 taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg               <  1:1827776/71‑1 (MQ=255)
                                                 taGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg               <  1:3340122/71‑1 (MQ=255)
                                                   gaagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg               <  1:1009229/69‑1 (MQ=255)
                                                     agagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTg            <  1:1623860/70‑1 (MQ=255)
                                                     agagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGa           <  1:1726669/71‑1 (MQ=255)
                                                           gAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGcc                     <  1:822839/55‑1 (MQ=255)
                                                           gAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGc     <  1:2603276/71‑1 (MQ=255)
                                                              ggCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGc     >  1:3044490/1‑68 (MQ=255)
                                                              ggCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAg  <  1:852350/71‑1 (MQ=255)
                                                              |                                                                      
CCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAG  >  minE/2595549‑2595681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: