| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,701 | G→T | 7.5% | A299A (GCC→GCA) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,701 | 0 | G | T | 7.5% | 307.3 / 6.5 | 93 | A299A (GCC→GCA) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (40/46); new base T (5/2); total (45/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
GAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGC > minE/2595632‑2595771 | gAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:3456834/71‑1 (MQ=255)gAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:423525/71‑1 (MQ=255) gCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa > 1:1946222/1‑71 (MQ=255) cGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCAt > 1:3066564/1‑71 (MQ=255) cgcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc > 1:3282149/1‑71 (MQ=255) gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:199052/1‑71 (MQ=255) gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:3539259/1‑71 (MQ=255) cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc < 1:1792587/71‑1 (MQ=255) ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCa < 1:3492741/71‑1 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:297205/1‑71 (MQ=255) caGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCAt < 1:474277/71‑1 (MQ=255) aGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCAt > 1:428902/1‑70 (MQ=255) aGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATa < 1:3038533/71‑1 (MQ=255) gCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa > 1:3596029/1‑71 (MQ=255) cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac > 1:1374281/1‑70 (MQ=255) cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac > 1:1839571/1‑70 (MQ=255) cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac < 1:3877988/70‑1 (MQ=255) ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc < 1:2052569/55‑1 (MQ=255) ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc < 1:371222/55‑1 (MQ=255) ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac < 1:183548/71‑1 (MQ=255) tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg < 1:3393457/55‑1 (MQ=255) tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac > 1:3244518/1‑70 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:1896605/1‑59 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa < 1:781785/64‑1 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccacc < 1:788161/70‑1 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac > 1:1268399/1‑69 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac > 1:1480340/1‑69 (MQ=255) tCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca < 1:1247776/70‑1 (MQ=255) tCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca < 1:3002096/70‑1 (MQ=255) ccGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAg < 1:2011710/55‑1 (MQ=255) ccGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa > 1:1934540/1‑63 (MQ=255) cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa > 1:3093729/1‑70 (MQ=255) cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa > 1:3222186/1‑70 (MQ=255) gCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccacc > 1:2045037/1‑66 (MQ=255) ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa > 1:2179942/1‑70 (MQ=255) cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAgg > 1:386941/1‑52 (MQ=255) gTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca < 1:1419730/59‑1 (MQ=255) gTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa > 1:271931/1‑63 (MQ=255) gTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcga < 1:381778/71‑1 (MQ=255) gTTGAGTACGCCAGAAAGATATTCCGTTAATCAGGCATTGCGCAGGCCATAAACCACCAGATACATGcc > 1:337736/1‑69 (MQ=255) tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATg > 1:3548705/1‑65 (MQ=255) gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCAt < 1:770288/47‑1 (MQ=255) aGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcg < 1:1017314/70‑1 (MQ=255) gcaCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGcc < 1:2402821/62‑1 (MQ=255) gTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag < 1:2219218/71‑1 (MQ=255) aCGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAg < 1:1029728/38‑1 (MQ=255) cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGAGagaag < 1:1168868/71‑1 (MQ=255) ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa > 1:1892086/1‑68 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt < 1:2421712/71‑1 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt < 1:3838769/71‑1 (MQ=255) cAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAACCCACCAGATACATGCcg < 1:3641748/59‑1 (MQ=255) cAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg < 1:636218/59‑1 (MQ=255) aGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAtaa > 1:346431/1‑69 (MQ=255) gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca > 1:726358/1‑46 (MQ=255) aaaGATATTCCGTTAATCCGTCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc > 1:2643353/1‑70 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCgaga > 1:3468182/1‑63 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCgaga > 1:182216/1‑63 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCgaga > 1:3053704/1‑63 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa < 1:1519251/64‑1 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc > 1:1498331/1‑70 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc > 1:2225303/1‑70 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:406610/71‑1 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:34104/71‑1 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:1708659/71‑1 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa > 1:449962/1‑71 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:582541/71‑1 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag > 1:1738566/1‑63 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt < 1:3340653/71‑1 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt < 1:1266934/71‑1 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt < 1:3062096/71‑1 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGAGAGAAGATGACAAt > 1:1714121/1‑71 (MQ=255) gATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca > 1:3139955/1‑42 (MQ=255) gATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca > 1:2788326/1‑42 (MQ=255) tatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt < 1:3226815/70‑1 (MQ=255) tactCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTg > 1:2525585/4‑71 (MQ=255) tactCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTg > 1:1357401/4‑71 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc < 1:2541932/71‑1 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:1969698/70‑1 (MQ=255) cGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCatg > 1:3096342/1‑69 (MQ=255) cGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCa < 1:551761/69‑1 (MQ=255) gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg < 1:3734046/71‑1 (MQ=255) gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg < 1:405584/69‑1 (MQ=255) ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc < 1:1228813/47‑1 (MQ=255) ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc < 1:603853/47‑1 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:472871/1‑71 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:2311898/1‑71 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg > 1:1300510/1‑70 (MQ=255) tAATACGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg > 1:1199977/1‑70 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACa < 1:3484224/37‑1 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACa < 1:3586714/37‑1 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACa < 1:2162589/37‑1 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:500776/70‑1 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:1630935/1‑70 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc < 1:3048356/71‑1 (MQ=255) cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc < 1:2819862/70‑1 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc > 1:548508/1‑71 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc < 1:551492/71‑1 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc > 1:2607462/1‑71 (MQ=255) | GAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGC > minE/2595632‑2595771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |