Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,725 C→G 12.5% L291L (CTG→CTC) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,7250CG12.5% 207.4 / 17.7 80L291L (CTG→CTC) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (30/40);  new base G (7/3);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCG  >  minE/2595656‑2595795
                                                                     |                                                                      
aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccacc                                                                        <  1:788161/70‑1 (MQ=255)
 tCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                                                       <  1:1247776/70‑1 (MQ=255)
 tCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                                                       <  1:3002096/70‑1 (MQ=255)
   cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                                     >  1:3222186/1‑70 (MQ=255)
   cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                                     >  1:3093729/1‑70 (MQ=255)
    gCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccacc                                                                        >  1:2045037/1‑66 (MQ=255)
     ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                                                                   >  1:2179942/1‑70 (MQ=255)
            gTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                                                       <  1:1419730/59‑1 (MQ=255)
            gTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                                                                   >  1:271931/1‑63 (MQ=255)
            gTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcga                                                           <  1:381778/71‑1 (MQ=255)
            gTTGAGTACGCCAGAAAGATATTCCGTTAATCAGGCATTGCGCAGGCCATAAACCACCAGATACATGcc                                                             >  1:337736/1‑69 (MQ=255)
              tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATg                                                               >  1:3548705/1‑65 (MQ=255)
                aGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcg                                                        <  1:1017314/70‑1 (MQ=255)
                 gcaCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGcc                                                             <  1:2402821/62‑1 (MQ=255)
                 gTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                                                      <  1:2219218/71‑1 (MQ=255)
                    cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGAGagaag                                                   <  1:1168868/71‑1 (MQ=255)
                      ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa                                                  >  1:1892086/1‑68 (MQ=255)
                      ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                 <  1:3838769/71‑1 (MQ=255)
                      ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                 <  1:2421712/71‑1 (MQ=255)
                       cAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAACCCACCAGATACATGCcg                                                            <  1:3641748/59‑1 (MQ=255)
                       cAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                                                            <  1:636218/59‑1 (MQ=255)
                        aGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAtaa                                               >  1:346431/1‑69 (MQ=255)
                         gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                                                       >  1:726358/1‑46 (MQ=255)
                          aaaGATATTCCGTTAATCCGTCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                              >  1:2643353/1‑70 (MQ=255)
                          aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCgaga                                                     >  1:182216/1‑63 (MQ=255)
                          aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCgaga                                                     >  1:3053704/1‑63 (MQ=255)
                          aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCgaga                                                     >  1:3468182/1‑63 (MQ=255)
                          aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                                    <  1:1519251/64‑1 (MQ=255)
                          aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                              >  1:2225303/1‑70 (MQ=255)
                          aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                              >  1:1498331/1‑70 (MQ=255)
                           aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                            <  1:1708659/71‑1 (MQ=255)
                           aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                            <  1:34104/71‑1 (MQ=255)
                           aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                            <  1:582541/71‑1 (MQ=255)
                           aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                            <  1:406610/71‑1 (MQ=255)
                           aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                            >  1:449962/1‑71 (MQ=255)
                            aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                                   >  1:1738566/1‑63 (MQ=255)
                            aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                           <  1:3340653/71‑1 (MQ=255)
                            aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                           <  1:1266934/71‑1 (MQ=255)
                            aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                           <  1:3062096/71‑1 (MQ=255)
                            aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGAGAGAAGATGACAAt                                           >  1:1714121/1‑71 (MQ=255)
                             gATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                                                       >  1:2788326/1‑42 (MQ=255)
                             gATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                                                       >  1:3139955/1‑42 (MQ=255)
                               tatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt                                         <  1:3226815/70‑1 (MQ=255)
                               tactCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTg                                        >  1:1357401/4‑71 (MQ=255)
                               tactCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTg                                        >  1:2525585/4‑71 (MQ=255)
                                 ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                      <  1:2541932/71‑1 (MQ=255)
                                 ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                       <  1:1969698/70‑1 (MQ=255)
                                    cGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCatg                                   >  1:3096342/1‑69 (MQ=255)
                                    cGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCa                                     <  1:551761/69‑1 (MQ=255)
                                     gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg                                  <  1:3734046/71‑1 (MQ=255)
                                     gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                    <  1:405584/69‑1 (MQ=255)
                                      ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                                                         <  1:603853/47‑1 (MQ=255)
                                      ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                                                         <  1:1228813/47‑1 (MQ=255)
                                       tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                >  1:2311898/1‑71 (MQ=255)
                                       tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                >  1:472871/1‑71 (MQ=255)
                                       tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg                                 >  1:1300510/1‑70 (MQ=255)
                                       tAATACGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg                                 >  1:1199977/1‑70 (MQ=255)
                                        aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACa                                                                 <  1:3586714/37‑1 (MQ=255)
                                        aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACa                                                                 <  1:2162589/37‑1 (MQ=255)
                                        aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACa                                                                 <  1:3484224/37‑1 (MQ=255)
                                        aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                >  1:1630935/1‑70 (MQ=255)
                                        aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                <  1:500776/70‑1 (MQ=255)
                                          tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                             <  1:3048356/71‑1 (MQ=255)
                                            cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                            <  1:2819862/70‑1 (MQ=255)
                                             ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                          <  1:551492/71‑1 (MQ=255)
                                             ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                          >  1:548508/1‑71 (MQ=255)
                                             ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                          >  1:2607462/1‑71 (MQ=255)
                                               cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                       <  1:1164393/56‑1 (MQ=255)
                                                aTTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                 <  1:1163289/45‑1 (MQ=255)
                                                aTTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                 <  1:2340247/45‑1 (MQ=255)
                                                   gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                           >  1:353962/1‑48 (MQ=255)
                                                   gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                           >  1:2624037/1‑48 (MQ=255)
                                                   gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg                     <  1:3652944/70‑1 (MQ=255)
                                                     gcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                   <  1:1644523/54‑1 (MQ=255)
                                                     gcAGGCCATAAACCACCAGATAAATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACt                  <  1:2089511/71‑1 (MQ=255)
                                                      cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt                 >  1:1379005/1‑71 (MQ=255)
                                                       aGGCCATAAACCACCAGATACATGCGGAGCGagaaga                                                  <  1:673405/37‑1 (MQ=255)
                                                       aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACttt                <  1:3576747/71‑1 (MQ=255)
                                                       aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTCCCAGGGGGCACCGCGCAGGACttt                <  1:3056390/71‑1 (MQ=255)
                                                         gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                            <  1:2817311/41‑1 (MQ=255)
                                                           cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc            <  1:3393576/71‑1 (MQ=255)
                                                             tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg           >  1:2769848/1‑70 (MQ=255)
                                                             tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg           >  1:3753967/1‑70 (MQ=255)
                                                             tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg           >  1:3751767/1‑70 (MQ=255)
                                                             tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg           >  1:3745906/1‑70 (MQ=255)
                                                              aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa         >  1:3490036/1‑71 (MQ=255)
                                                              aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa         >  1:1123326/1‑71 (MQ=255)
                                                               aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                    >  1:2332685/1‑43 (MQ=255)
                                                                accaccAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                      >  1:1237171/1‑40 (MQ=255)
                                                                 ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                     >  1:86078/1‑40 (MQ=255)
                                                                 ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                     >  1:1073704/1‑40 (MQ=255)
                                                                   accaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa     >  1:3168596/1‑70 (MQ=255)
                                                                     caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg  >  1:44901/1‑71 (MQ=255)
                                                                     |                                                                      
ATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCG  >  minE/2595656‑2595795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: