| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,749 | G→A | 14.5% | V283V (GTC→GTT) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,749 | 0 | G | A | 14.5% | 191.2 / 20.7 | 76 | V283V (GTC→GTT) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (31/34); new base A (8/3); total (39/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
AAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACA > minE/2595682‑2595818 | aaaGATATTCCGTTAATCCGTCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc > 1:2643353/1‑70 (MQ=255)aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc > 1:2225303/1‑70 (MQ=255)aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc > 1:1498331/1‑70 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:582541/71‑1 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:34104/71‑1 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:406610/71‑1 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa > 1:449962/1‑71 (MQ=255) aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:1708659/71‑1 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt < 1:3340653/71‑1 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt < 1:3062096/71‑1 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt < 1:1266934/71‑1 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGAGAGAAGATGACAAt > 1:1714121/1‑71 (MQ=255) tatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt < 1:3226815/70‑1 (MQ=255) tactCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTg > 1:1357401/4‑71 (MQ=255) tactCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTg > 1:2525585/4‑71 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc < 1:2541932/71‑1 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:1969698/70‑1 (MQ=255) cGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCatg > 1:3096342/1‑69 (MQ=255) cGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCa < 1:551761/69‑1 (MQ=255) gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg < 1:3734046/71‑1 (MQ=255) gTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg < 1:405584/69‑1 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:472871/1‑71 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:2311898/1‑71 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg > 1:1300510/1‑70 (MQ=255) tAATACGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg > 1:1199977/1‑70 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:1630935/1‑70 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:500776/70‑1 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc < 1:3048356/71‑1 (MQ=255) cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc < 1:2819862/70‑1 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc > 1:548508/1‑71 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc > 1:2607462/1‑71 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc < 1:551492/71‑1 (MQ=255) cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:1164393/56‑1 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt > 1:353962/1‑48 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt > 1:2624037/1‑48 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg < 1:3652944/70‑1 (MQ=255) gcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg < 1:1644523/54‑1 (MQ=255) gcAGGCCATAAACCACCAGATAAATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACt < 1:2089511/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt > 1:1379005/1‑71 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACttt < 1:3576747/71‑1 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTCCCAGGGGGCACCGCGCAGGACttt < 1:3056390/71‑1 (MQ=255) gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa < 1:2817311/41‑1 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc < 1:3393576/71‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:3753967/1‑70 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:3751767/1‑70 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:3745906/1‑70 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:2769848/1‑70 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:3490036/1‑71 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:1123326/1‑71 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg > 1:2332685/1‑43 (MQ=255) accaccAGATACATGCCGAGCGAGAAGATGACAATCTGcc > 1:1237171/1‑40 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:86078/1‑40 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:1073704/1‑40 (MQ=255) accaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:3168596/1‑70 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg > 1:44901/1‑71 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc < 1:2151999/71‑1 (MQ=255) gtACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGt > 1:708703/2‑60 (MQ=255) aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc < 1:827032/35‑1 (MQ=255) aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcc > 1:2399177/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg < 1:799009/71‑1 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc < 1:1591466/70‑1 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt < 1:3763092/70‑1 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt < 1:2215868/71‑1 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:293720/1‑70 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:248896/1‑70 (MQ=255) gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc > 1:3911088/3‑70 (MQ=255) agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa < 1:2783976/50‑1 (MQ=255) agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:577750/71‑1 (MQ=255) agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:3111723/71‑1 (MQ=255) gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc > 1:3068514/1‑41 (MQ=255) gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga > 1:2588677/1‑71 (MQ=255) gaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCg < 1:1367792/53‑1 (MQ=255) aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac < 1:3075628/71‑1 (MQ=255) agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac > 1:2945395/1‑70 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt < 1:3286558/62‑1 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAg > 1:1551908/1‑64 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa > 1:3884291/1‑70 (MQ=255) | AAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACA > minE/2595682‑2595818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |