Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,764 G→C 11.1% A278A (GCC→GCG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,7640GC11.1% 168.5 / 10.5 63A278A (GCC→GCGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (24/32);  new base C (3/4);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

TAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCG  >  minE/2595695‑2595831
                                                                     |                                                                   
tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                                    >  1:2311898/1‑71 (MQ=255)
tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                                    >  1:472871/1‑71 (MQ=255)
tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg                                                                     >  1:1300510/1‑70 (MQ=255)
tAATACGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg                                                                     >  1:1199977/1‑70 (MQ=255)
 aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                                    <  1:500776/70‑1 (MQ=255)
 aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                                    >  1:1630935/1‑70 (MQ=255)
   tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                                                 <  1:3048356/71‑1 (MQ=255)
     cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                                                <  1:2819862/70‑1 (MQ=255)
      ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                                              >  1:2607462/1‑71 (MQ=255)
      ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                                              <  1:551492/71‑1 (MQ=255)
      ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                                              >  1:548508/1‑71 (MQ=255)
            gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg                                                         <  1:3652944/70‑1 (MQ=255)
              gcAGGCCATAAACCACCAGATAAATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACt                                                      <  1:2089511/71‑1 (MQ=255)
               cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt                                                     >  1:1379005/1‑71 (MQ=255)
                aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACttt                                                    <  1:3576747/71‑1 (MQ=255)
                aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTCCCAGGGGGCACCGCGCAGGACttt                                                    <  1:3056390/71‑1 (MQ=255)
                    cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc                                                <  1:3393576/71‑1 (MQ=255)
                      tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                               >  1:2769848/1‑70 (MQ=255)
                      tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                               >  1:3745906/1‑70 (MQ=255)
                      tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                               >  1:3751767/1‑70 (MQ=255)
                      tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                               >  1:3753967/1‑70 (MQ=255)
                       aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa                                             >  1:1123326/1‑71 (MQ=255)
                       aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa                                             >  1:3490036/1‑71 (MQ=255)
                            accaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                         >  1:3168596/1‑70 (MQ=255)
                              caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                      >  1:44901/1‑71 (MQ=255)
                               aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                     <  1:2151999/71‑1 (MQ=255)
                                 gtACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGt                                              >  1:708703/2‑60 (MQ=255)
                                     aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                                   <  1:827032/35‑1 (MQ=255)
                                     aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcc                               >  1:2399177/1‑71 (MQ=255)
                                        ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg                            <  1:799009/71‑1 (MQ=255)
                                        ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc                             <  1:1591466/70‑1 (MQ=255)
                                          gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                           <  1:3763092/70‑1 (MQ=255)
                                           agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt                         <  1:2215868/71‑1 (MQ=255)
                                           agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                          >  1:248896/1‑70 (MQ=255)
                                           agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                          >  1:293720/1‑70 (MQ=255)
                                            gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc                        >  1:3911088/3‑70 (MQ=255)
                                               agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                          <  1:2783976/50‑1 (MQ=255)
                                               agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg                     <  1:3111723/71‑1 (MQ=255)
                                               agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg                     <  1:577750/71‑1 (MQ=255)
                                                gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc                                                  >  1:3068514/1‑41 (MQ=255)
                                                gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                    >  1:2588677/1‑71 (MQ=255)
                                                gaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCg                                      <  1:1367792/53‑1 (MQ=255)
                                                 aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                   <  1:3075628/71‑1 (MQ=255)
                                                  agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                   >  1:2945395/1‑70 (MQ=255)
                                                    aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt                         <  1:3286558/62‑1 (MQ=255)
                                                    aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAg                       >  1:1551908/1‑64 (MQ=255)
                                                      gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa               >  1:3884291/1‑70 (MQ=255)
                                                       acgaTCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaa              <  1:3209329/67‑1 (MQ=255)
                                                       aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa             >  1:3276071/1‑71 (MQ=255)
                                                       aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa             >  1:3030770/1‑71 (MQ=255)
                                                        cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAAt                                        >  1:709522/1‑43 (MQ=255)
                                                        cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                          <  1:3750632/57‑1 (MQ=255)
                                                        cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa             <  1:3873666/70‑1 (MQ=255)
                                                        cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat            <  1:947737/71‑1 (MQ=255)
                                                        cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat            <  1:1788310/71‑1 (MQ=255)
                                                         aaTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                           <  1:1194399/55‑1 (MQ=255)
                                                          aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata           <  1:2337450/70‑1 (MQ=255)
                                                           tCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGAcc                               <  1:1960919/49‑1 (MQ=255)
                                                            cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata        >  1:2126632/1‑71 (MQ=255)
                                                             tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                   <  1:2454054/59‑1 (MQ=255)
                                                              gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                <  1:534959/61‑1 (MQ=255)
                                                              gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                <  1:539648/61‑1 (MQ=255)
                                                              gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat       >  1:1796150/1‑70 (MQ=255)
                                                               ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaaca           <  1:246383/65‑3 (MQ=255)
                                                               ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa     <  1:2974521/71‑1 (MQ=255)
                                                               ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa     <  1:577785/71‑1 (MQ=255)
                                                               ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa     <  1:734774/71‑1 (MQ=255)
                                                                 aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                 <  1:3726970/41‑1 (MQ=255)
                                                                 aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTAAGCGACGACAAATAATATCAgc   >  1:2681529/1‑71 (MQ=255)
                                                                  gggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcg  >  1:3489709/1‑71 (MQ=255)
                                                                    gggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc   <  1:1207702/68‑1 (MQ=255)
                                                                     ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat         <  1:424520/61‑1 (MQ=255)
                                                                     ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat         <  1:3326233/61‑1 (MQ=255)
                                                                     ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat         <  1:3330795/61‑1 (MQ=255)
                                                                     |                                                                   
TAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCG  >  minE/2595695‑2595831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: