Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,595,764 | G→C | 11.1% | A278A (GCC→GCG) | arsB ← | arsenite/antimonite transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,595,764 | 0 | G | C | 11.1% | 168.5 / 10.5 | 63 | A278A (GCC→GCG) | arsB | arsenite/antimonite transporter |
Reads supporting (aligned to +/- strand): ref base G (24/32); new base C (3/4); total (27/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01 |
TAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCG > minE/2595695‑2595831 | tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:2311898/1‑71 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:472871/1‑71 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg > 1:1300510/1‑70 (MQ=255) tAATACGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgggg > 1:1199977/1‑70 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:500776/70‑1 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:1630935/1‑70 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc < 1:3048356/71‑1 (MQ=255) cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc < 1:2819862/70‑1 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc > 1:2607462/1‑71 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc < 1:551492/71‑1 (MQ=255) ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc > 1:548508/1‑71 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg < 1:3652944/70‑1 (MQ=255) gcAGGCCATAAACCACCAGATAAATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACt < 1:2089511/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt > 1:1379005/1‑71 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACttt < 1:3576747/71‑1 (MQ=255) aGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTCCCAGGGGGCACCGCGCAGGACttt < 1:3056390/71‑1 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc < 1:3393576/71‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:2769848/1‑70 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:3745906/1‑70 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:3751767/1‑70 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg > 1:3753967/1‑70 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:1123326/1‑71 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa > 1:3490036/1‑71 (MQ=255) accaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:3168596/1‑70 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg > 1:44901/1‑71 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc < 1:2151999/71‑1 (MQ=255) gtACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGt > 1:708703/2‑60 (MQ=255) aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc < 1:827032/35‑1 (MQ=255) aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcc > 1:2399177/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg < 1:799009/71‑1 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc < 1:1591466/70‑1 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt < 1:3763092/70‑1 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt < 1:2215868/71‑1 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:248896/1‑70 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:293720/1‑70 (MQ=255) gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc > 1:3911088/3‑70 (MQ=255) agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa < 1:2783976/50‑1 (MQ=255) agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:3111723/71‑1 (MQ=255) agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:577750/71‑1 (MQ=255) gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc > 1:3068514/1‑41 (MQ=255) gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga > 1:2588677/1‑71 (MQ=255) gaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCg < 1:1367792/53‑1 (MQ=255) aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac < 1:3075628/71‑1 (MQ=255) agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac > 1:2945395/1‑70 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt < 1:3286558/62‑1 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAg > 1:1551908/1‑64 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa > 1:3884291/1‑70 (MQ=255) acgaTCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaa < 1:3209329/67‑1 (MQ=255) aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3276071/1‑71 (MQ=255) aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3030770/1‑71 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAAt > 1:709522/1‑43 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt < 1:3750632/57‑1 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa < 1:3873666/70‑1 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat < 1:947737/71‑1 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat < 1:1788310/71‑1 (MQ=255) aaTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt < 1:1194399/55‑1 (MQ=255) aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata < 1:2337450/70‑1 (MQ=255) tCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGAcc < 1:1960919/49‑1 (MQ=255) cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata > 1:2126632/1‑71 (MQ=255) tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac < 1:2454054/59‑1 (MQ=255) gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac < 1:534959/61‑1 (MQ=255) gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac < 1:539648/61‑1 (MQ=255) gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat > 1:1796150/1‑70 (MQ=255) ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaaca < 1:246383/65‑3 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa < 1:2974521/71‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa < 1:577785/71‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa < 1:734774/71‑1 (MQ=255) aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa < 1:3726970/41‑1 (MQ=255) aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTAAGCGACGACAAATAATATCAgc > 1:2681529/1‑71 (MQ=255) gggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcg > 1:3489709/1‑71 (MQ=255) gggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc < 1:1207702/68‑1 (MQ=255) ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:424520/61‑1 (MQ=255) ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:3326233/61‑1 (MQ=255) ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:3330795/61‑1 (MQ=255) | TAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCG > minE/2595695‑2595831 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |