Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,785 C→G 9.1% T271T (ACG→ACC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,7850CG 9.1% 219.3 / 10.1 77T271T (ACG→ACCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (36/34);  new base G (3/4);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

CATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGG  >  minE/2595715‑2595849
                                                                      |                                                                
cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc                                                                  <  1:3393576/71‑1 (MQ=255)
  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                                                 >  1:2769848/1‑70 (MQ=255)
  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                                                 >  1:3753967/1‑70 (MQ=255)
  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                                                 >  1:3751767/1‑70 (MQ=255)
  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                                                 >  1:3745906/1‑70 (MQ=255)
   aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa                                                               >  1:1123326/1‑71 (MQ=255)
   aaaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa                                                               >  1:3490036/1‑71 (MQ=255)
        accaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                                           >  1:3168596/1‑70 (MQ=255)
          caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                                        >  1:44901/1‑71 (MQ=255)
           aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                                       <  1:2151999/71‑1 (MQ=255)
             gtACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGt                                                                >  1:708703/2‑60 (MQ=255)
                 aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcc                                                 >  1:2399177/1‑71 (MQ=255)
                    ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg                                              <  1:799009/71‑1 (MQ=255)
                    ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc                                               <  1:1591466/70‑1 (MQ=255)
                      gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                             <  1:3763092/70‑1 (MQ=255)
                       agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt                                           <  1:2215868/71‑1 (MQ=255)
                       agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                            >  1:293720/1‑70 (MQ=255)
                       agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                            >  1:248896/1‑70 (MQ=255)
                        gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc                                          >  1:3911088/3‑70 (MQ=255)
                           agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                                            <  1:2783976/50‑1 (MQ=255)
                           agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg                                       <  1:577750/71‑1 (MQ=255)
                           agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg                                       <  1:3111723/71‑1 (MQ=255)
                            gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                      >  1:2588677/1‑71 (MQ=255)
                            gaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCg                                                        <  1:1367792/53‑1 (MQ=255)
                             aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                     <  1:3075628/71‑1 (MQ=255)
                              agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                     >  1:2945395/1‑70 (MQ=255)
                                aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt                                           <  1:3286558/62‑1 (MQ=255)
                                aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                         >  1:1551908/1‑64 (MQ=255)
                                  gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                 >  1:3884291/1‑70 (MQ=255)
                                   acgaTCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaa                                <  1:3209329/67‑1 (MQ=255)
                                   aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                               >  1:3030770/1‑71 (MQ=255)
                                   aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                               >  1:3276071/1‑71 (MQ=255)
                                    cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAAt                                                          >  1:709522/1‑43 (MQ=255)
                                    cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                            <  1:3750632/57‑1 (MQ=255)
                                    cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                               <  1:3873666/70‑1 (MQ=255)
                                    cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                              <  1:1788310/71‑1 (MQ=255)
                                    cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                              <  1:947737/71‑1 (MQ=255)
                                     aaTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                             <  1:1194399/55‑1 (MQ=255)
                                      aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata                             <  1:2337450/70‑1 (MQ=255)
                                       tCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGAcc                                                 <  1:1960919/49‑1 (MQ=255)
                                        cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata                          >  1:2126632/1‑71 (MQ=255)
                                         tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                     <  1:2454054/59‑1 (MQ=255)
                                          gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                  <  1:534959/61‑1 (MQ=255)
                                          gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                  <  1:539648/61‑1 (MQ=255)
                                          gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                         >  1:1796150/1‑70 (MQ=255)
                                           ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaaca                             <  1:246383/65‑3 (MQ=255)
                                           ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                       <  1:2974521/71‑1 (MQ=255)
                                           ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                       <  1:734774/71‑1 (MQ=255)
                                           ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                       <  1:577785/71‑1 (MQ=255)
                                             aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                                   <  1:3726970/41‑1 (MQ=255)
                                             aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTAAGCGACGACAAATAATATCAgc                     >  1:2681529/1‑71 (MQ=255)
                                              gggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcg                    >  1:3489709/1‑71 (MQ=255)
                                                gggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc                     <  1:1207702/68‑1 (MQ=255)
                                                 ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                           <  1:3330795/61‑1 (MQ=255)
                                                 ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                           <  1:3326233/61‑1 (MQ=255)
                                                 ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                           <  1:424520/61‑1 (MQ=255)
                                                  gCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcctc                                               <  1:888881/40‑3 (MQ=255)
                                                  gCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                           <  1:1945520/60‑1 (MQ=255)
                                                     ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca              >  1:1246929/1‑70 (MQ=255)
                                                     ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca              >  1:2460449/1‑70 (MQ=255)
                                                      cgcgCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                      >  1:2441214/1‑45 (MQ=255)
                                                       gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                  >  1:3567531/1‑48 (MQ=255)
                                                           aGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                               <  1:1289216/47‑1 (MQ=255)
                                                            ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                      >  1:945989/1‑39 (MQ=255)
                                                            ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCa      <  1:3384986/71‑1 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                         >  1:3893393/1‑35 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                         >  1:211432/1‑35 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                         >  1:3897988/1‑35 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga                                   >  1:2747616/1‑41 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGcc                >  1:1338743/1‑60 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa     >  1:2484311/1‑71 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa     <  1:3076638/71‑1 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa     >  1:793823/1‑71 (MQ=255)
                                                             gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa     >  1:805595/1‑71 (MQ=255)
                                                                 ttACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc                     >  1:96538/1‑51 (MQ=255)
                                                                  tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata                             >  1:1809748/1‑42 (MQ=255)
                                                                  tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATgg  <  1:523107/69‑1 (MQ=255)
                                                                   aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                         <  1:116830/45‑1 (MQ=255)
                                                                      |                                                                
CATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGG  >  minE/2595715‑2595849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: