Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,791 A→G 8.0% I269I (ATT→ATC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,7910AG 8.0% 208.6 / 7.8 75I269I (ATT→ATCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (34/35);  new base G (2/4);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

ACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGG  >  minE/2595723‑2595859
                                                                    |                                                                    
accaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                                                     >  1:3168596/1‑70 (MQ=255)
  caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                                                  >  1:44901/1‑71 (MQ=255)
   aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                                                 <  1:2151999/71‑1 (MQ=255)
         aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcc                                                           >  1:2399177/1‑71 (MQ=255)
            ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg                                                        <  1:799009/71‑1 (MQ=255)
            ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc                                                         <  1:1591466/70‑1 (MQ=255)
              gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                       <  1:3763092/70‑1 (MQ=255)
               agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt                                                     <  1:2215868/71‑1 (MQ=255)
               agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                      >  1:248896/1‑70 (MQ=255)
               agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                      >  1:293720/1‑70 (MQ=255)
                gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc                                                    >  1:3911088/3‑70 (MQ=255)
                   agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                                                      <  1:2783976/50‑1 (MQ=255)
                   agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg                                                 <  1:577750/71‑1 (MQ=255)
                   agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg                                                 <  1:3111723/71‑1 (MQ=255)
                    gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                                >  1:2588677/1‑71 (MQ=255)
                    gaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCg                                                                  <  1:1367792/53‑1 (MQ=255)
                     aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                               <  1:3075628/71‑1 (MQ=255)
                      agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                               >  1:2945395/1‑70 (MQ=255)
                        aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt                                                     <  1:3286558/62‑1 (MQ=255)
                        aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                                   >  1:1551908/1‑64 (MQ=255)
                          gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                           >  1:3884291/1‑70 (MQ=255)
                           acgaTCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaa                                          <  1:3209329/67‑1 (MQ=255)
                           aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                         >  1:3030770/1‑71 (MQ=255)
                           aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                         >  1:3276071/1‑71 (MQ=255)
                            cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAAt                                                                    >  1:709522/1‑43 (MQ=255)
                            cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                      <  1:3750632/57‑1 (MQ=255)
                            cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                         <  1:3873666/70‑1 (MQ=255)
                            cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                                        <  1:1788310/71‑1 (MQ=255)
                            cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                                        <  1:947737/71‑1 (MQ=255)
                             aaTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                       <  1:1194399/55‑1 (MQ=255)
                              aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata                                       <  1:2337450/70‑1 (MQ=255)
                               tCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGAcc                                                           <  1:1960919/49‑1 (MQ=255)
                                cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                    >  1:2126632/1‑71 (MQ=255)
                                 tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                               <  1:2454054/59‑1 (MQ=255)
                                  gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                            <  1:534959/61‑1 (MQ=255)
                                  gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                            <  1:539648/61‑1 (MQ=255)
                                  gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                                   >  1:1796150/1‑70 (MQ=255)
                                   ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaaca                                       <  1:246383/65‑3 (MQ=255)
                                   ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                                 <  1:577785/71‑1 (MQ=255)
                                   ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                                 <  1:2974521/71‑1 (MQ=255)
                                   ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                                 <  1:734774/71‑1 (MQ=255)
                                     aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                                             <  1:3726970/41‑1 (MQ=255)
                                     aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTAAGCGACGACAAATAATATCAgc                               >  1:2681529/1‑71 (MQ=255)
                                      gggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcg                              >  1:3489709/1‑71 (MQ=255)
                                        gggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc                               <  1:1207702/68‑1 (MQ=255)
                                         ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                     <  1:424520/61‑1 (MQ=255)
                                         ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                     <  1:3326233/61‑1 (MQ=255)
                                         ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                     <  1:3330795/61‑1 (MQ=255)
                                          gCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcctc                                                         <  1:888881/40‑3 (MQ=255)
                                          gCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                     <  1:1945520/60‑1 (MQ=255)
                                             ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                        >  1:2460449/1‑70 (MQ=255)
                                             ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                        >  1:1246929/1‑70 (MQ=255)
                                              cgcgCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                                >  1:2441214/1‑45 (MQ=255)
                                               gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                            >  1:3567531/1‑48 (MQ=255)
                                                   aGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                         <  1:1289216/47‑1 (MQ=255)
                                                    ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                                >  1:945989/1‑39 (MQ=255)
                                                    ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCa                <  1:3384986/71‑1 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                                   >  1:3897988/1‑35 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                                   >  1:3893393/1‑35 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                                   >  1:211432/1‑35 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga                                             >  1:2747616/1‑41 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGcc                          >  1:1338743/1‑60 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa               >  1:793823/1‑71 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa               >  1:2484311/1‑71 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa               >  1:805595/1‑71 (MQ=255)
                                                     gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa               <  1:3076638/71‑1 (MQ=255)
                                                         ttACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc                               >  1:96538/1‑51 (MQ=255)
                                                          tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata                                       >  1:1809748/1‑42 (MQ=255)
                                                          tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATgg            <  1:523107/69‑1 (MQ=255)
                                                           aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                                   <  1:116830/45‑1 (MQ=255)
                                                               gTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCAc     <  1:719969/71‑1 (MQ=255)
                                                                tATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCAcc    >  1:2532380/1‑71 (MQ=255)
                                                                 aTTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg   >  1:3369482/1‑71 (MQ=255)
                                                                  ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCgg  <  1:114036/71‑1 (MQ=255)
                                                                  ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg   >  1:95249/1‑70 (MQ=255)
                                                                   tAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATgg            >  1:342415/1‑60 (MQ=255)
                                                                   tAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCgg  >  1:671239/1‑70 (MQ=255)
                                                                    |                                                                    
ACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGG  >  minE/2595723‑2595859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: