Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,595,796 | C→A | 6.7% | A268S (GCG→TCG) | arsB ← | arsenite/antimonite transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,595,796 | 0 | C | A | 6.7% | 242.7 / 2.6 | 74 | A268S (GCG→TCG) | arsB | arsenite/antimonite transporter |
Reads supporting (aligned to +/- strand): ref base C (33/36); new base A (2/3); total (35/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 |
AGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGCCG > minE/2595726‑2595866 | aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc < 1:2151999/71‑1 (MQ=255) aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcc > 1:2399177/1‑71 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg < 1:799009/71‑1 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc < 1:1591466/70‑1 (MQ=255) gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt < 1:3763092/70‑1 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt < 1:2215868/71‑1 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:293720/1‑70 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:248896/1‑70 (MQ=255) gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc > 1:3911088/3‑70 (MQ=255) agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:3111723/71‑1 (MQ=255) agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg < 1:577750/71‑1 (MQ=255) gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga > 1:2588677/1‑71 (MQ=255) aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac < 1:3075628/71‑1 (MQ=255) agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac > 1:2945395/1‑70 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt < 1:3286558/62‑1 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAg > 1:1551908/1‑64 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa > 1:3884291/1‑70 (MQ=255) acgaTCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaa < 1:3209329/67‑1 (MQ=255) aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3030770/1‑71 (MQ=255) aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3276071/1‑71 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt < 1:3750632/57‑1 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa < 1:3873666/70‑1 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat < 1:1788310/71‑1 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat < 1:947737/71‑1 (MQ=255) aaTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt < 1:1194399/55‑1 (MQ=255) aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata < 1:2337450/70‑1 (MQ=255) tCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGAcc < 1:1960919/49‑1 (MQ=255) cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata > 1:2126632/1‑71 (MQ=255) tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac < 1:2454054/59‑1 (MQ=255) gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac < 1:534959/61‑1 (MQ=255) gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac < 1:539648/61‑1 (MQ=255) gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat > 1:1796150/1‑70 (MQ=255) ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaaca < 1:246383/65‑3 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa < 1:734774/71‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa < 1:577785/71‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa < 1:2974521/71‑1 (MQ=255) aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa < 1:3726970/41‑1 (MQ=255) aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTAAGCGACGACAAATAATATCAgc > 1:2681529/1‑71 (MQ=255) gggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcg > 1:3489709/1‑71 (MQ=255) gggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc < 1:1207702/68‑1 (MQ=255) ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:424520/61‑1 (MQ=255) ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:3326233/61‑1 (MQ=255) ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:3330795/61‑1 (MQ=255) gCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcctc < 1:888881/40‑3 (MQ=255) gCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:1945520/60‑1 (MQ=255) ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca > 1:1246929/1‑70 (MQ=255) ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca > 1:2460449/1‑70 (MQ=255) cgcgCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga > 1:2441214/1‑45 (MQ=255) gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac > 1:3567531/1‑48 (MQ=255) aGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa < 1:1289216/47‑1 (MQ=255) ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga > 1:945989/1‑39 (MQ=255) ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCa < 1:3384986/71‑1 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAg > 1:3897988/1‑35 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAg > 1:3893393/1‑35 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAg > 1:211432/1‑35 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga > 1:2747616/1‑41 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGcc > 1:1338743/1‑60 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa > 1:805595/1‑71 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa < 1:3076638/71‑1 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa > 1:2484311/1‑71 (MQ=255) gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa > 1:793823/1‑71 (MQ=255) ttACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc > 1:96538/1‑51 (MQ=255) tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata > 1:1809748/1‑42 (MQ=255) tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATgg < 1:523107/69‑1 (MQ=255) aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat < 1:116830/45‑1 (MQ=255) gTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCAc < 1:719969/71‑1 (MQ=255) tATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCAcc > 1:2532380/1‑71 (MQ=255) aTTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg > 1:3369482/1‑71 (MQ=255) ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCgg < 1:114036/71‑1 (MQ=255) ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg > 1:95249/1‑70 (MQ=255) tAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATgg > 1:342415/1‑60 (MQ=255) tAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCgg > 1:671239/1‑70 (MQ=255) aTCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGaa < 1:1412381/70‑1 (MQ=255) tCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACATCTGCAATGGCGCTCACCGGAATg < 1:615194/71‑1 (MQ=255) gCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGcc > 1:2900866/1‑71 (MQ=255) cATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGcc < 1:1439812/70‑1 (MQ=255) cATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGCCg < 1:1361385/71‑1 (MQ=255) | AGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGCCG > minE/2595726‑2595866 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |