Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,796 C→A 6.7% A268S (GCG→TCG)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,7960CA 6.7% 242.7 / 2.6 74A268S (GCG→TCG) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (33/36);  new base A (2/3);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

AGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGCCG  >  minE/2595726‑2595866
                                                                      |                                                                      
aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                                                        <  1:2151999/71‑1 (MQ=255)
      aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcc                                                                  >  1:2399177/1‑71 (MQ=255)
         ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg                                                               <  1:799009/71‑1 (MQ=255)
         ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc                                                                <  1:1591466/70‑1 (MQ=255)
           gagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                              <  1:3763092/70‑1 (MQ=255)
            agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt                                                            <  1:2215868/71‑1 (MQ=255)
            agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                             >  1:293720/1‑70 (MQ=255)
            agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                             >  1:248896/1‑70 (MQ=255)
             gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc                                                           >  1:3911088/3‑70 (MQ=255)
                agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg                                                        <  1:3111723/71‑1 (MQ=255)
                agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcg                                                        <  1:577750/71‑1 (MQ=255)
                 gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                                       >  1:2588677/1‑71 (MQ=255)
                  aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                                      <  1:3075628/71‑1 (MQ=255)
                   agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                                      >  1:2945395/1‑70 (MQ=255)
                     aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGttt                                                            <  1:3286558/62‑1 (MQ=255)
                     aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                                          >  1:1551908/1‑64 (MQ=255)
                       gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                                  >  1:3884291/1‑70 (MQ=255)
                        acgaTCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaa                                                 <  1:3209329/67‑1 (MQ=255)
                        aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                                >  1:3030770/1‑71 (MQ=255)
                        aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                                >  1:3276071/1‑71 (MQ=255)
                         cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                             <  1:3750632/57‑1 (MQ=255)
                         cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                                <  1:3873666/70‑1 (MQ=255)
                         cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                                               <  1:1788310/71‑1 (MQ=255)
                         cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                                               <  1:947737/71‑1 (MQ=255)
                          aaTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                              <  1:1194399/55‑1 (MQ=255)
                           aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata                                              <  1:2337450/70‑1 (MQ=255)
                            tCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGAcc                                                                  <  1:1960919/49‑1 (MQ=255)
                             cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                           >  1:2126632/1‑71 (MQ=255)
                              tGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                                      <  1:2454054/59‑1 (MQ=255)
                               gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                                   <  1:534959/61‑1 (MQ=255)
                               gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                                   <  1:539648/61‑1 (MQ=255)
                               gCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                                          >  1:1796150/1‑70 (MQ=255)
                                ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaaca                                              <  1:246383/65‑3 (MQ=255)
                                ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                                        <  1:734774/71‑1 (MQ=255)
                                ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                                        <  1:577785/71‑1 (MQ=255)
                                ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCa                                        <  1:2974521/71‑1 (MQ=255)
                                  aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                                                    <  1:3726970/41‑1 (MQ=255)
                                  aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTAAGCGACGACAAATAATATCAgc                                      >  1:2681529/1‑71 (MQ=255)
                                   gggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcg                                     >  1:3489709/1‑71 (MQ=255)
                                     gggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc                                      <  1:1207702/68‑1 (MQ=255)
                                      ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                            <  1:424520/61‑1 (MQ=255)
                                      ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                            <  1:3326233/61‑1 (MQ=255)
                                      ggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                            <  1:3330795/61‑1 (MQ=255)
                                       gCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcctc                                                                <  1:888881/40‑3 (MQ=255)
                                       gCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                            <  1:1945520/60‑1 (MQ=255)
                                          ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                               >  1:1246929/1‑70 (MQ=255)
                                          ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                               >  1:2460449/1‑70 (MQ=255)
                                           cgcgCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                                       >  1:2441214/1‑45 (MQ=255)
                                            gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                                   >  1:3567531/1‑48 (MQ=255)
                                                aGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                                <  1:1289216/47‑1 (MQ=255)
                                                 ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                                       >  1:945989/1‑39 (MQ=255)
                                                 ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCa                       <  1:3384986/71‑1 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                                          >  1:3897988/1‑35 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                                          >  1:3893393/1‑35 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAg                                                          >  1:211432/1‑35 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga                                                    >  1:2747616/1‑41 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGcc                                 >  1:1338743/1‑60 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa                      >  1:805595/1‑71 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa                      <  1:3076638/71‑1 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa                      >  1:2484311/1‑71 (MQ=255)
                                                  gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa                      >  1:793823/1‑71 (MQ=255)
                                                      ttACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgc                                      >  1:96538/1‑51 (MQ=255)
                                                       tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaata                                              >  1:1809748/1‑42 (MQ=255)
                                                       tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATgg                   <  1:523107/69‑1 (MQ=255)
                                                        aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                                          <  1:116830/45‑1 (MQ=255)
                                                            gTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCAc            <  1:719969/71‑1 (MQ=255)
                                                             tATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCAcc           >  1:2532380/1‑71 (MQ=255)
                                                              aTTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg          >  1:3369482/1‑71 (MQ=255)
                                                               ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCgg         <  1:114036/71‑1 (MQ=255)
                                                               ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg          >  1:95249/1‑70 (MQ=255)
                                                                tAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATgg                   >  1:342415/1‑60 (MQ=255)
                                                                tAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCgg         >  1:671239/1‑70 (MQ=255)
                                                                  aTCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGaa       <  1:1412381/70‑1 (MQ=255)
                                                                   tCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACATCTGCAATGGCGCTCACCGGAATg     <  1:615194/71‑1 (MQ=255)
                                                                     gCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGcc   >  1:2900866/1‑71 (MQ=255)
                                                                      cATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGcc   <  1:1439812/70‑1 (MQ=255)
                                                                      cATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGCCg  <  1:1361385/71‑1 (MQ=255)
                                                                      |                                                                      
AGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGGAATGCCG  >  minE/2595726‑2595866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: