Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 311,787 G→C 14.8% F362L (TTC→TTG) ‡ acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE311,7870GC14.8% 233.2 / 24.4 81F362L (TTC→TTG) ‡acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (30/39);  new base C (8/4);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTT  >  minE/311718‑311857
                                                                     |                                                                      
ggcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGGCGGAATCAACGTCGCGCGGa                                                                       <  1:2395896/71‑1 (MQ=255)
    gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt                                                                   <  1:2214056/71‑1 (MQ=255)
    gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt                                                                   <  1:869617/71‑1 (MQ=255)
    gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt                                                                   <  1:1888094/71‑1 (MQ=255)
      aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGTCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt                                                                 <  1:75692/71‑1 (MQ=255)
      aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt                                                                 <  1:1157785/71‑1 (MQ=255)
      aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt                                                                 <  1:96267/71‑1 (MQ=255)
      aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt                                                                 >  1:2433711/1‑71 (MQ=255)
      aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt                                                                 >  1:173217/1‑71 (MQ=255)
        ggACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg                                                                >  1:1101363/1‑70 (MQ=255)
         gACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg                                                                >  1:3538603/1‑69 (MQ=255)
         gACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCa                                                              <  1:980473/71‑1 (MQ=255)
          aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg                                                             >  1:2153029/1‑71 (MQ=255)
           cGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg                                                             >  1:1111932/1‑70 (MQ=255)
             gCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa                                                          <  1:2798570/71‑1 (MQ=255)
                aaGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa                                                          >  1:98439/1‑68 (MQ=255)
                aaGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa                                                          >  1:92396/1‑68 (MQ=255)
                 aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt                                                                   <  1:1603528/58‑1 (MQ=255)
                  ggTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGAt                                                     <  1:2509573/71‑1 (MQ=255)
                      ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCa                                                              >  1:1460361/1‑58 (MQ=255)
                      ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCa                                                              >  1:3491954/1‑58 (MQ=255)
                      ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt                                                 <  1:2069847/71‑1 (MQ=255)
                      ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt                                                 <  1:1341076/71‑1 (MQ=255)
                      ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt                                                 <  1:3224834/71‑1 (MQ=255)
                       cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt                                                 >  1:1429650/1‑70 (MQ=255)
                       cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa                                                <  1:3872070/71‑1 (MQ=255)
                        gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCTCGCGGAAGTTCTGCAGGAACAGATACATaa                                               >  1:119129/1‑71 (MQ=255)
                        gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa                                               >  1:3168855/1‑71 (MQ=255)
                        gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa                                               >  1:2923309/1‑71 (MQ=255)
                        gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa                                               >  1:1819928/1‑71 (MQ=255)
                        gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa                                               >  1:3833828/1‑71 (MQ=255)
                         aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc                                              >  1:3808043/3‑71 (MQ=2)
                         aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc                                              >  1:571554/3‑71 (MQ=2)
                         aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc                                              >  1:1167036/3‑71 (MQ=2)
                         aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa                                                                      >  1:2579188/1‑47 (MQ=255)
                         aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa                                                                      >  1:1292292/1‑47 (MQ=255)
                         aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc                                              <  1:1282483/71‑1 (MQ=255)
                         aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc                                              >  1:2781428/1‑71 (MQ=255)
                          gCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc                                              >  1:3407060/1‑70 (MQ=255)
                          acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc                                              <  1:1004605/69‑1 (MQ=2)
                           cAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc                                              >  1:542339/1‑69 (MQ=255)
                           cAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCa                                            <  1:1035660/71‑1 (MQ=255)
                           cAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc                                              >  1:861068/1‑69 (MQ=2)
                              tACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa                                                  <  1:2039763/62‑1 (MQ=255)
                              caccacgGGTACCGCAATGGTGGGGATCAGCGTTTCTCGCATATTCTGCAAGAACAGATACATCACCAgg                                          >  1:198794/2‑70 (MQ=9)
                              caccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGa                                         <  1:1335111/70‑1 (MQ=9)
                              caccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGa                                         <  1:1648415/70‑1 (MQ=9)
                                ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa                                        >  1:2530623/1‑70 (MQ=255)
                                ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa                                        >  1:1643001/1‑70 (MQ=255)
                                ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac                                       <  1:2281146/71‑1 (MQ=255)
                                 caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa                                        >  1:2588772/1‑69 (MQ=255)
                                  accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac                                     <  1:1917300/71‑1 (MQ=255)
                                  accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACAGGAacac                                     >  1:376607/1‑70 (MQ=255)
                                   ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGa                                         <  1:600954/66‑1 (MQ=255)
                                   ccGGTACGCCAATGGTCGGAATCAACGTCGCGCgg                                                                        <  1:1916262/35‑1 (MQ=255)
                                    cGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGt                                                                    <  1:3039447/38‑1 (MQ=255)
                                    cGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac                                     >  1:3109271/1‑69 (MQ=255)
                                         ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat                               <  1:3386862/68‑1 (MQ=9)
                                         cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg                                          <  1:3751181/59‑1 (MQ=255)
                                         cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg                              <  1:2025039/71‑1 (MQ=255)
                                          ggCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatga                             <  1:361876/71‑1 (MQ=255)
                                          cgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcataa                             >  1:363357/2‑69 (MQ=9)
                                           gCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACAtca                                               >  1:726613/1‑50 (MQ=2)
                                            cAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATc                           >  1:2326278/1‑71 (MQ=255)
                                             aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATc                           <  1:1798241/70‑1 (MQ=255)
                                             aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCg                          <  1:1150541/71‑1 (MQ=255)
                                              aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                                        <  1:2911672/40‑1 (MQ=255)
                                               tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCt                        >  1:207882/1‑71 (MQ=255)
                                                ggTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCtt                       >  1:3127576/1‑71 (MQ=2)
                                                  tCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa                                                  <  1:148009/42‑1 (MQ=255)
                                                  tCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCg                     <  1:2622455/71‑1 (MQ=255)
                                                      aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg                                    <  1:432043/52‑1 (MQ=255)
                                                         cAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg                                          <  1:2055783/43‑1 (MQ=255)
                                                         cAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg                                          <  1:2428334/43‑1 (MQ=255)
                                                          aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa                                        <  1:3018264/44‑1 (MQ=255)
                                                          aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg              <  1:3276463/70‑1 (MQ=255)
                                                           aCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat                            >  1:3532092/1‑55 (MQ=255)
                                                           aCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtt            <  1:884341/71‑1 (MQ=255)
                                                            cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt           <  1:3800745/71‑1 (MQ=255)
                                                             gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAg                                           >  1:239906/1‑38 (MQ=255)
                                                              tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa         <  1:2852763/71‑1 (MQ=255)
                                                                  gcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg              <  1:1585492/62‑1 (MQ=255)
                                                                     gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa         <  1:568395/64‑1 (MQ=255)
                                                                     gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACtt  >  1:2785062/1‑71 (MQ=255)
                                                                     gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACtt  <  1:1908208/71‑1 (MQ=255)
                                                                     gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACt   >  1:243692/1‑70 (MQ=255)
                                                                     |                                                                      
GGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTT  >  minE/311718‑311857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: