| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,596,130 | G→A | 26.5% | V156V (GTC→GTT) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,596,130 | 0 | G | A | 26.5% | 108.9 / 39.4 | 64 | V156V (GTC→GTT) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (21/26); new base A (7/10); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCT > minE/2596062‑2596199 | aTCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc < 1:855573/71‑1 (MQ=255) tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc > 1:228468/1‑70 (MQ=255) ccGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa < 1:1929019/71‑1 (MQ=255) cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATaa < 1:453881/71‑1 (MQ=255) gAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg > 1:1513322/1‑71 (MQ=255) aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAgg > 1:1371377/1‑71 (MQ=255) cgcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTg > 1:274491/1‑71 (MQ=255) gcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg > 1:1117304/1‑71 (MQ=255) gcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg < 1:1896383/71‑1 (MQ=255) cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc < 1:2733741/53‑1 (MQ=255) cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc < 1:2492156/53‑1 (MQ=255) cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGAc < 1:2396547/53‑1 (MQ=255) gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg < 1:2789780/71‑1 (MQ=255) aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGc > 1:744467/1‑58 (MQ=255) aaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg > 1:2877939/1‑70 (MQ=255) aaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg > 1:635676/1‑70 (MQ=255) aaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg > 1:2715622/1‑70 (MQ=255) aaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGt < 1:203151/71‑1 (MQ=255) gCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTAt < 1:2529413/71‑1 (MQ=255) ccGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATc > 1:3677093/1‑71 (MQ=255) cGAGGCCAAAGAAATCAGCGTAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCg < 1:3030136/71‑1 (MQ=255) aGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:1731717/71‑1 (MQ=255) gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCaa > 1:2084086/1‑71 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:2061612/71‑1 (MQ=255) ccAAAGAAATAAGCGGAAACGATATTCACCAGGTTGGAGAc < 1:1967548/41‑1 (MQ=255) cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTa < 1:2746529/60‑1 (MQ=255) gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACaa > 1:239345/2‑41 (MQ=12) gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAAtg < 1:881907/42‑2 (MQ=16) gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa < 1:1599426/70‑3 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATaa < 1:2609176/44‑1 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATaa < 1:2669200/44‑1 (MQ=255) aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATaaa < 1:2122001/71‑1 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGgaga > 1:1524877/1‑37 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa > 1:333578/1‑71 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa > 1:3449102/1‑71 (MQ=255) aGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATct > 1:675806/1‑61 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg > 1:3522283/1‑56 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt > 1:3510425/1‑71 (MQ=255) cGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgc > 1:70191/1‑70 (MQ=255) ggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGTTATCGGCAATGAATCCGGCCGCCa > 1:3568100/1‑71 (MQ=255) gaCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAtga < 1:2985791/70‑3 (MQ=255) aCGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgcc > 1:56520/1‑63 (MQ=255) aCGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCAc < 1:1297938/71‑1 (MQ=255) aCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa > 1:2573727/1‑53 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAt < 1:2702354/51‑1 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:2419478/71‑3 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAca < 1:1542783/70‑2 (MQ=255) tatTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAAc > 1:2097649/1‑71 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGt < 1:3217631/38‑1 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCAc < 1:487693/65‑1 (MQ=255) tCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGaa < 1:3465117/67‑1 (MQ=255) tCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:3485191/1‑70 (MQ=255) aCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCg < 1:3889235/51‑1 (MQ=255) aCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCa < 1:819963/70‑1 (MQ=255) ccAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATcc > 1:991115/1‑49 (MQ=255) ccAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgt < 1:307056/71‑2 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgcc < 1:416266/55‑1 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:3783559/71‑1 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTgca > 1:3436126/1‑50 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgt > 1:2497266/1‑71 (MQ=255) ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc > 1:3315800/1‑70 (MQ=255) gTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:1555969/39‑1 (MQ=255) gTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGc < 1:955960/36‑1 (MQ=12) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAg > 1:1119177/1‑68 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg < 1:2402450/70‑1 (MQ=255) gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGc < 1:2267886/70‑1 (MQ=255) agaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCgg < 1:58925/42‑1 (MQ=255) agaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCt < 1:1856390/71‑1 (MQ=255) gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGt < 1:3057455/63‑1 (MQ=255) | ATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCT > minE/2596062‑2596199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |