| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,596,178 | C→G | 14.5% | V140V (GTG→GTC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,596,178 | 0 | C | G | 14.5% | 127.3 / 13.9 | 55 | V140V (GTG→GTC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (20/27); new base G (4/4); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01 | |||||||||||
ACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATCGGTGTCAAA > minE/2596110‑2596247 | aCGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCAc < 1:1297938/71‑1 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:2419478/71‑3 (MQ=255) gATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAca < 1:1542783/70‑2 (MQ=255) tatTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAAc > 1:2097649/1‑71 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCAc < 1:487693/65‑1 (MQ=255) tCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGaa < 1:3465117/67‑1 (MQ=255) tCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc > 1:3485191/1‑70 (MQ=255) aCCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCa < 1:819963/70‑1 (MQ=255) ccAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgt < 1:307056/71‑2 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:3783559/71‑1 (MQ=255) aGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgt > 1:2497266/1‑71 (MQ=255) ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc > 1:3315800/1‑70 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAg > 1:1119177/1‑68 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTg < 1:2402450/70‑1 (MQ=255) gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGc < 1:2267886/70‑1 (MQ=255) agaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCt < 1:1856390/71‑1 (MQ=255) aaaCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACATAGGCCAGCGTCGTGccc > 1:548452/3‑70 (MQ=255) gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGt < 1:3057455/63‑1 (MQ=255) aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGccctg < 1:2091714/71‑4 (MQ=255) aaTAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGcc < 1:2481318/66‑1 (MQ=255) aTAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt < 1:868664/71‑1 (MQ=255) gCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAc < 1:3213076/71‑1 (MQ=255) gCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt > 1:1278145/1‑64 (MQ=255) gCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAccc > 1:2921311/1‑70 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAAcc > 1:1064160/1‑68 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:988578/71‑1 (MQ=255) cAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:857920/71‑1 (MQ=255) aGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa > 1:2518166/1‑70 (MQ=255) ggCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa > 1:2130549/1‑69 (MQ=255) cTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGc < 1:3818098/71‑1 (MQ=255) cGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCcag > 1:2087774/1‑70 (MQ=255) ggCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCAt < 1:2851223/69‑1 (MQ=255) gCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGc < 1:44490/71‑1 (MQ=255) gCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGc > 1:1089642/1‑71 (MQ=255) cAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCg < 1:891881/71‑1 (MQ=255) aaTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGa > 1:1419448/1‑71 (MQ=255) aaTGAATCAGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGa > 1:1457080/1‑71 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt < 1:3698868/44‑1 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACt < 1:848298/44‑1 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt > 1:3428531/1‑71 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt > 1:94696/1‑71 (MQ=255) aTGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt > 1:138630/1‑71 (MQ=255) gAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCcag > 1:3607990/1‑57 (MQ=255) aaTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGAc < 1:257306/71‑1 (MQ=255) aTCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGc > 1:2076347/1‑64 (MQ=255) cGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATc < 1:1870091/71‑1 (MQ=255) cgccATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:2485358/41‑1 (MQ=255) cATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGAt < 1:2019084/57‑1 (MQ=255) aTCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGc > 1:860097/1‑41 (MQ=255) aTCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATCGGTGTCaa < 1:1472319/71‑1 (MQ=255) aTCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATCGGTGTCa > 1:3835687/1‑70 (MQ=255) aTCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATCGGTGTCa > 1:1787107/1‑70 (MQ=255) tCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTa < 1:1069713/36‑1 (MQ=255) tCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGa > 1:115348/1‑57 (MQ=255) tCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATCGGTGTCaaa < 1:2241733/71‑1 (MQ=255) | ACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTTTACTGAACCCTAAAGCCAGCAGCATGGCGATGACAATCGGTGTCAAA > minE/2596110‑2596247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |